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Papers
61,005 resultsShowing papers similar to Evaluation of Digestion Methods in Microplastic Recovery from Mussels (Mytilus galloprovincialis) for a Standardised Microplastic Isolation Protocol
ClearOil extraction following digestion to separate microplastics from mussels
This study compared several chemical digestion and extraction methods for isolating microplastics from mussel tissue, finding that hydrogen peroxide digestion followed by oil-based extraction achieved over 95% recovery across multiple plastic types. Optimized extraction methods are essential for accurately detecting microplastic contamination in shellfish that humans consume.
Testing a Method for Quantifying Microplastic Content in Mytilus californianus
Researchers tested a combined potassium hydroxide digestion and ultrasonic bath method for isolating and quantifying microplastics from California mussel (Mytilus californianus) tissues, exposing mussels in 20-gallon tanks to controlled MP concentrations and evaluating recovery efficiency.
Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.
Optimization of a new multi-reagent procedure for quantitative mussel digestion in microplastic analysis
This study optimized a chemical digestion protocol for extracting microplastics from mussel tissue by testing combinations of multiple reagents, aiming to completely destroy biological material while preserving plastic particles for analysis. The optimized method improved microplastic recovery compared to single-reagent approaches. Accurate extraction methods are critical for reliably measuring microplastic contamination in mussels, a widely consumed shellfish and important food safety indicator.
Microplastics in wild mussels (Mytilus spp.) from the north coast of Spain
Mussels from two regions of northern Spain's coastline contained microplastics, with potassium hydroxide digestion recovering significantly more particles than nitric acid digestion. The choice of digestion method substantially affects the number of microplastics detected in seafood, making methodological standardization critical for food safety assessments.
Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
An interlaboratory comparison tested two methods for extracting microplastics from fish gastrointestinal tracts and mussel tissue: 15% hydrogen peroxide digestion and 10% potassium hydroxide digestion. The exercise, conducted across four labs within the Plastic Busters MPAs project, aimed to harmonize extraction protocols for more comparable results.
Evaluation of existing methods to extract microplastics from bivalve tissue: Adapted KOH digestion protocol improves filtration at single-digit pore size
Researchers compared several existing methods for extracting microplastics from bivalve tissue, including hydrogen peroxide, enzymatic digestion, and potassium hydroxide treatments. They found that only the KOH method allowed filtration through very fine filters down to 1.2 micrometers when a neutralization step was added, enabling recovery of much smaller microplastics. The study recommends KOH digestion as the most practical extraction method for studies aiming to assess human exposure risk from consuming shellfish.
Selection and optimization of a protocol for extraction of microplastics from Mactra veneriformis
Researchers tested and optimized a protocol for extracting microplastics from the commercial clam Mactra veneriformis, finding 10% potassium hydroxide (KOH) was the best tissue-digesting agent while preserving plastic particles. The validated method was then applied to assess real-world microplastic contamination in commercially sold clams.
Extraction and identification of microplastics from mussels: Method development and preliminary results
Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.
Prevalence of Microplastics in the Eastern Oyster Crassostrea virginica in the Chesapeake Bay: The Impact of Different Digestion Methods on Microplastic Properties
Eastern oysters from three Chesapeake Bay sites were found to contain microplastics, with hydrogen peroxide and potassium hydroxide digestion methods yielding the highest recovery rates, while nitric acid produced satisfactory results with better microplastic preservation.
Microplastic Polymer Mass Fractions in Marine Bivalves: From Isolation to Hazard Risk
This review examines methods for isolating and quantifying microplastics in marine bivalves such as mussels and oysters, focusing on efficient digestion procedures and detection techniques. The study highlights microwave-assisted methods and pyrolysis gas chromatography-mass spectrometry as promising approaches for determining polymer mass fractions, and discusses the health risks associated with microplastic contamination in seafood consumed by humans.
Proposed validation stages for MPs extraction from edible mussels (Mytilus galloprovincialis)
Researchers developed and validated a new method for extracting microplastics from edible mussels using enzymes and surfactants instead of mechanical agitation. The technique achieved high recovery rates while preserving the chemical integrity and color of the microplastic particles, making it easier to accurately identify and characterize the types of plastics found in seafood.
Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus
This study evaluated and optimized laboratory extraction methods for isolating and identifying microplastics from the tissues of green mussels, which accumulate plastic particles through their filter-feeding habits. Reliable extraction methods are essential for accurately quantifying microplastic contamination in shellfish consumed by humans.
Assessing the feasibility of Fenton's reagent for microplastic extraction from mussels and its application to coastal pollution monitoring in Zhejiang, China.
Researchers validated Fenton's reagent as an efficient protocol for extracting microplastics from mussel tissue, achieving over 88% digestion efficiency without altering particle morphology, then applied it to coastal Zhejiang samples finding 1.8–9.0 microplastics per gram wet weight dominated by chlorinated polyethylene and polyvinyl chloride particles smaller than 50 μm.
Microplastics on plankton samples: Multiple digestion techniques assessment based on weight, size, and FTIR spectroscopy analyses
This study compared KOH, hydrogen peroxide, and two-step digestion protocols for removing organic matter from plankton samples before microplastic analysis, finding that two-step protocols improved recovery but that all methods caused measurable changes in microplastic weight and size, necessitating protocol standardization.
Rapid optimization of decapod crustacean digestion for efficient microplastic extraction (MPs)
Researchers developed and compared four digestion protocols for extracting microplastics from commercial crab samples, evaluating potassium hydroxide thermo-alkaline digestion, hydrogen peroxide oxidative digestion, protease enzymatic digestion, and dodecyl dimethyl betaine surfactant digestion. Protocols 2 and 4 achieved digestion efficiencies of 93% and 96% respectively, with good recovery rates and preservation of microplastic chemical integrity confirmed via FTIR.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
Exposure of Mytilus galloprovincialis to Microplastics: Accumulation, Depuration and Evaluation of the Expression Levels of a Selection of Molecular Biomarkers
Researchers exposed Mediterranean mussels to a realistic mixture of microplastic types and then tested whether a standard purification process could remove them. They found that purification significantly reduced microplastic contamination in the mussels and that molecular biomarkers in the gills could detect the biological effects of exposure. The study suggests that both purification protocols and molecular monitoring tools could help address microplastic risks in farmed shellfish.
Reliable methodologies to determine microplastics in mussels: Enhanced digestion protocols, transference to gold-coated filters and determination via laser-based transflectance infrared spectrometry
Researchers compared six digestion protocols for extracting microplastics from Mediterranean mussels (Mytilus galloprovincialis) and found enzymatic-oxidative digestion achieved the highest recovery (>90%), while a two-step alkaline-surfactant method offered a faster, cost-effective alternative. They also validated a transfer protocol to gold-coated filters for quantum cascade laser infrared analysis.
Sample decomposition to determine microplastics in parts of mullets
Researchers developed an optimized potassium hydroxide digestion method for extracting microplastics from different tissues of mullet fish, including meat, skin, and the digestive system. Different tissues required different digestion conditions to achieve effective extraction without destroying the plastic particles. The method improves the accuracy of microplastic detection in fish commonly consumed by people.
Methodology optimization to quantify microplastic presence in planktonic copepods, chaetognaths and fish larvae
Researchers optimized a hydrogen peroxide digestion method for extracting and counting microplastics from zooplankton and fish larvae while preserving the physical and chemical integrity of the plastic particles, achieving over 85% recovery across 13 plastic types. Having a reliable, standardized method for quantifying microplastics in plankton is essential for accurately assessing how much plastic is entering marine food webs at the base of the food chain.
A short review on the recent method development for extraction and identification of microplastics in mussels and fish, two major groups of seafood
This review summarized the methods used to extract and identify microplastics in mussels and fish, two major seafood groups. Researchers found that alkaline digestion was the most common extraction technique while FTIR spectroscopy was the predominant identification method. The study recommends standardizing analytical protocols to enable better comparison of microplastic contamination data across different studies of seafood.
Concentration of microplastics in bivalves of the environment: a systematic review
Microplastics were found in bivalves across 70 species in 36 countries, with mussels (Mytilus spp.) and oysters (Crassostrea spp.) most studied, but inconsistent digestion and identification methodologies make meaningful cross-study comparisons of contamination levels difficult.
A glimpse into the future: A suitable methodological approach for the detection and identification of micro-bioplastics in biota
Researchers developed and validated a chemical digestion method using potassium hydroxide to extract and identify micro-bioplastics made from Mater-Bi in mussel tissue, comparing virgin and aged forms. The study demonstrated that this adapted protocol offers a viable methodological approach for detecting bio-microplastics in marine biota as bioplastic alternatives to conventional plastics become more prevalent.