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Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Environmental Toxicology and Chemistry 2016 317 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Ana I. Catarino, Theodore B. Henry, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Ana I. Catarino, Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Ana I. Catarino, Richard C. Thompson Richard C. Thompson Ana I. Catarino, Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Theodore B. Henry, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Theodore B. Henry, Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Theodore B. Henry, Ana I. Catarino, Theodore B. Henry, Ana I. Catarino, Ana I. Catarino, Theodore B. Henry, Theodore B. Henry, Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson William Sanderson, William Sanderson, Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Theodore B. Henry, Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Theodore B. Henry, Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Theodore B. Henry, Richard C. Thompson Theodore B. Henry, Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson William Sanderson, Richard C. Thompson Theodore B. Henry, Theodore B. Henry, Richard C. Thompson Ana I. Catarino, Theodore B. Henry, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson Ana I. Catarino, Richard C. Thompson Ana I. Catarino, Ana I. Catarino, Ana I. Catarino, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson Theodore B. Henry, Richard C. Thompson Richard C. Thompson Ana I. Catarino, Richard C. Thompson Richard C. Thompson Richard C. Thompson Richard C. Thompson

Summary

Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.

Models

Invertebrate other

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO<sub>3</sub> , and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO<sub>3</sub> caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. Environ Toxicol Chem 2017;36:947-951. © 2016 SETAC.

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