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Article ? AI-assigned paper type based on the abstract. Classification may not be perfect — flag errors using the feedback button. Tier 2 ? Original research — experimental, observational, or case-control study. Direct primary evidence. Food & Water Gut & Microbiome Human Health Effects Marine & Wildlife Policy & Risk Sign in to save

Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Environmental Toxicology and Chemistry 2016 317 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Ana I. Catarino, Richard C. Thompson, William Sanderson, Theodore B. Henry

Summary

Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.

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Invertebrate other

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3 , and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. Environ Toxicol Chem 2017;36:947-951. © 2016 SETAC.

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