We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Selection and optimization of a protocol for extraction of microplastics from Mactra veneriformis
AI summary Read the abstract
Researchers tested and optimized a protocol for extracting microplastics from the commercial clam Mactra veneriformis, finding 10% potassium hydroxide (KOH) was the best tissue-digesting agent while preserving plastic particles. The validated method was then applied to assess real-world microplastic contamination in commercially sold clams.
Nowadays, several digestion protocols have been employed to extract microplastics from marine biota. However, the appropriate protocol and its optimal operating conditions to eliminate the clam Mactra veneriformis tissues have never been investigated. In this study, two synchronous phases were taken to select the compromise elimination protocol which was efficient in digesting biological materials with little effect on identification of the tested plastic polymers. Furthermore, the protocol from compromising results between two phases was optimized by an orthogonal experiment to determine the best operating conditions. Then, an application of the optimization protocol to investigate microplastics pollution in commercial clams was conducted. According to our results, 10% KOH showed fairly well digestion efficiency and little effect on identification of the tested microplastics. Furthermore, best operating conditions were demonstrated as treating clam tissues with 1:5 (M:V) of 10% KOH solution, incubating at 60 °C, shaking at 30 rpm for 12 h. In China, commercial clams were found contaminated with widespread microplastics pollution (3.50 ± 1.35 items/g). These problems should be further investigated and assessed due to the increased consumption as seafoods.
More Papers Like This
Evaluation of existing methods to extract microplastics from bivalve tissue: Adapted KOH digestion protocol improves filtration at single-digit pore size
AI summary Read the abstract
Researchers compared several existing methods for extracting microplastics from bivalve tissue, including hydrogen peroxide, enzymatic digestion, and potassium hydroxide treatments. They found that only the KOH method allowed filtration through very fine filters down to 1.2 micrometers when a neutralization step was added, enabling recovery of much smaller microplastics. The study recommends KOH digestion as the most practical extraction method for studies aiming to assess human exposure risk from consuming shellfish.
Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
AI summary Read the abstract
Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.
Methodology for microplastics quantification in clams
AI summary Read the abstract
Researchers developed a simplified methodology for extracting and quantifying microplastics from clam tissue, addressing the need for standardized protocols to assess microplastic accumulation in seafood that poses direct human exposure risk when consumed whole.
Metode ekstrakcije mikroplastike iz školjkaša
AI summary Read the abstract
This paper reviewed and compared methods for extracting microplastics from bivalves such as mussels and clams, assessing the efficiency and reliability of different digestion and separation protocols. Robust extraction methods are essential for accurately measuring microplastic contamination in commercially important shellfish.
The Recovery of Microplastics from Rock Oysters Using Digestion Method
AI summary Read the abstract
Researchers tested three chemical digestion methods to extract microplastics from rock oyster tissue, comparing potassium hydroxide alone with combinations including hydrogen peroxide and sodium hypochlorite. Optimizing extraction methods for shellfish tissue is important because inconsistent protocols make it hard to compare microplastic contamination levels across different studies.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.