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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Detection Methods
Gut & Microbiome
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Microplastics on plankton samples: Multiple digestion techniques assessment based on weight, size, and FTIR spectroscopy analyses
Marine Pollution Bulletin2021
61 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 45
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Atsuhiko Isobe
María Belén Alfonso,
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
María Belén Alfonso,
María Belén Alfonso,
María Belén Alfonso,
María Belén Alfonso,
María Belén Alfonso,
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
María Belén Alfonso,
María Belén Alfonso,
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
María Belén Alfonso,
Atsuhiko Isobe
Kayoko Takashima,
María Belén Alfonso,
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
María Belén Alfonso,
Atsuhiko Isobe
María Belén Alfonso,
María Belén Alfonso,
María Belén Alfonso,
Kayoko Takashima,
Atsuhiko Isobe
María Belén Alfonso,
Atsuhiko Isobe
Sayaka Yamaguchi,
María Belén Alfonso,
María Belén Alfonso,
Mie Tanaka,
Mie Tanaka,
María Belén Alfonso,
Atsuhiko Isobe
Mie Tanaka,
Mie Tanaka,
Atsuhiko Isobe
María Belén Alfonso,
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Atsuhiko Isobe
Summary
This study compared KOH, hydrogen peroxide, and two-step digestion protocols for removing organic matter from plankton samples before microplastic analysis, finding that two-step protocols improved recovery but that all methods caused measurable changes in microplastic weight and size, necessitating protocol standardization.
Digestion protocols are needed to determine microplastics abundance and features. This study assessed the organic matter (OM) digestion efficiency on plankton samples and the MPs' weight, size, and polymer changes under different digestion techniques. For this, 2-step (KOH and H<sub>2</sub>O<sub>2</sub> + Fe<sup>2+</sup>) and 3-step (2-step and enzymes) digestion techniques were assessed under different duration and temperature conditions. The results obtained for OM digestion with 2-step and 3-step techniques were satisfactory. Weight changes were registered for polyethylene terephthalate (PET), polystyrene foam, polyvinyl chloride, and polycarbonate with 2-step digestion, but with inconsistent values. Significant size changes were registered only for PET applying 2-step digestion techniques at 60 °C. Using 40 °C for 72 h prevailed all polymers from size changes. Polyethylene weathered MPs were also preserved, including an enzymatic step. Polymer fingerprints were not affected by any digestion technique. Based on these results, any method applying high temperatures will damage MPs.