Papers

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Article Tier 2

Preliminary Study on the Toxic Effects of Polystyrene Microplastics in Human Colorectal Cells

Researchers tested the toxic effects of polystyrene microplastics on human colorectal cells in the laboratory and found that both 80-nanometer and 500-nanometer particles significantly reduced cell viability and induced programmed cell death. The effects were size- and concentration-dependent, with smaller particles generally causing greater toxicity, providing experimental evidence for evaluating the intestinal health risks of microplastic exposure.

2026 Springer Link (Chiba Institute of Technology)
Article Tier 2

The effects of polystyrene microplastics on human intestinal cells health and function

This study examined how polystyrene microplastics affect normal and cancer intestinal cells, addressing a gap left by previous research that used only cancer cell lines and pristine plastics. The work evaluated microplastic toxicity under more realistic conditions including digestive system biotransformation, assessing effects on nutrient uptake and cellular function.

2022 1 citations
Article Tier 2

Preliminary Study on the Toxic Effects of Polystyrene Microplastics in Human Colorectal Cells

Researchers evaluated the toxic effects of polystyrene microplastics in two sizes, 80 nanometers and 500 nanometers, on human colorectal cells in laboratory culture. They found that both sizes significantly reduced cell viability, induced cell death, and disrupted the normal cell cycle in a dose-dependent manner. The study provides preliminary evidence that microplastic particles at sizes relevant to human exposure may pose risks to intestinal cell health.

2026 E3S Web of Conferences
Article Tier 2

Polystyrene nanoplastics exposure causes inflammation and death of esophageal cell

Researchers exposed human esophageal cells to polystyrene nanoplastics and found that the particles triggered significant inflammation and cell death. The nanoplastics activated inflammatory signaling pathways and caused oxidative damage to the cells at concentrations relevant to human dietary exposure. The findings raise concerns about the potential effects of nanoplastic contamination in food and drinking water on the upper digestive tract.

2023 Ecotoxicology and Environmental Safety 20 citations
Article Tier 2

Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice

Researchers exposed mice to polystyrene microplastics for six weeks and found that the particles accumulated in the gut, reduced protective mucus secretion, and damaged the intestinal barrier. The microplastics also significantly altered the composition of gut bacteria, decreasing beneficial species and increasing harmful ones. The study suggests that microplastic ingestion could disrupt gut health in mammals by simultaneously impairing the physical barrier and reshaping the microbiome.

2018 The Science of The Total Environment 986 citations
Article Tier 2

Polystyrene microplastics aggravate inflammatory damage in mice with intestinal immune imbalance

Researchers found that polystyrene microplastics caused significantly worse inflammatory damage in mice that already had compromised intestinal immune systems compared to healthy mice. The microplastics increased inflammatory markers, disrupted gut bacteria, and caused more severe tissue damage in the vulnerable animals. The study suggests that individuals with pre-existing gut health issues may be more susceptible to the harmful effects of microplastic exposure.

2022 The Science of The Total Environment 153 citations
Article Tier 2

Elucidating the Size‐Dependency of In Vitro Digested Polystyrene Microplastics on Human Intestinal Cells Health and Function

Polystyrene microplastics of different sizes were subjected to simulated in vitro digestion and then applied to human intestinal cells, with smaller particles causing greater disruption to cell health and barrier function than larger ones. The results suggest that the smallest microplastics reaching the human gut pose the greatest risk to intestinal integrity.

2022 Macromolecular Chemistry and Physics 19 citations
Article Tier 2

Microplastics cross the murine intestine and induce inflammatory cell death after phagocytosis by human monocytes and neutrophils

Researchers administered polystyrene microplastics orally to mice and then assessed distribution and immune cell interactions in both mice and human cells. Both 1 µm and 10 µm particles crossed the intestinal epithelium and were detected in blood and liver after 10 days, and human monocytes and neutrophils that ingested the particles underwent inflammatory cell death.

2025
Article Tier 2

Influence of Microplastics on Morphological Manifestations of Experimental Acute Colitis

Researchers fed polystyrene microplastics to mice for six weeks and found that healthy mice developed changes in their colon lining, including altered mucus composition and immune cell populations. When mice with experimentally induced colitis also consumed microplastics, their intestinal inflammation was significantly more severe. The study suggests that microplastic exposure may worsen inflammatory bowel conditions.

2023 Toxics 28 citations
Article Tier 2

Potential toxicity of polystyrene microplastic particles

Researchers investigated the cellular-level toxicity of polystyrene microplastic particles and found that they stimulated immune responses in a size- and concentration-dependent manner. The particles triggered the production of cytokines and chemokines, which are signaling molecules involved in inflammation. The study challenges the common assumption that microplastics pose minimal risk to human health, suggesting they may have immunological effects upon direct contact with cells.

2020 Scientific Reports 630 citations
Article Tier 2

Polystyrene microplastics induce endoplasmic reticulum stress, apoptosis and inflammation by disrupting the gut microbiota in carp intestines

Researchers fed carp polystyrene microplastics and found that the particles disrupted their gut bacteria, killing off beneficial species and promoting those linked to diseases. The microplastics triggered a stress response in intestinal cells that led to inflammation, cell death, and tissue damage. Since carp is a widely eaten fish, these gut health effects raise questions about how microplastics in aquatic environments could affect the safety of fish that humans consume.

2023 Environmental Pollution 94 citations
Article Tier 2

Polystyrene micro- and nanoplastics aggravates colitis in a mouse model – effects on biodistribution, macrophage polarization, and gut microbiome

Researchers found that polystyrene micro- and nanoplastics aggravated colitis symptoms in a mouse model, increasing gut permeability, inflammatory cytokine levels, and tissue damage compared to controls. The study provides mechanistic evidence linking microplastic exposure to worsening of inflammatory bowel conditions.

2024 Research Square (Research Square)
Article Tier 2

The potential effects of microplastic pollution on human digestive tract cells

Researchers tested polystyrene particles of four different sizes on human colon and small intestine cells to assess the potential effects of microplastic ingestion. They found that the smallest nanoscale particles were more readily taken up by cells and caused greater reductions in cell viability and increased oxidative stress. The study suggests that smaller plastic particles may pose a greater risk to the human digestive tract than larger ones.

2021 Chemosphere 143 citations
Article Tier 2

Polystyrene microplastics (PS-MPs) toxicity induced oxidative stress and intestinal injury in nematode Caenorhabditis elegans

Researchers exposed the nematode C. elegans to various concentrations of polystyrene microplastics and measured physiological, biochemical, and molecular responses. The study found that microplastics accumulated in the intestine and caused oxidative stress, intestinal injury, and adverse physiological effects at concentrations as low as 1 microgram per liter, suggesting that even low-level microplastic exposure can damage gut tissues.

2020 The Science of The Total Environment 201 citations
Article Tier 2

Polystyrene microplastics cross the murine intestine and induce inflammatory cell death after phagocytosis by human monocytes and neutrophils

Researchers orally administered 1 μm and 10 μm polystyrene particles to mice for 10 days and found that both sizes crossed the intestinal epithelium and were detected in blood and liver; when phagocytosed by human monocytes and neutrophils, the particles triggered complement-dependent inflammatory cell death.

2025 iScience
Article Tier 2

Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks

Researchers found that two weeks of oral polystyrene microplastic exposure in ICR mice induced an inflammatory response specifically in the mid colon, suggesting microplastics may contribute to colonic inflammation.

2021 Laboratory Animal Research 42 citations
Article Tier 2

Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs

Researchers found that polystyrene nanoplastics disrupt the gut lining in mice by altering tiny RNA molecules that control the production of protective proteins in the intestinal barrier. The nanoplastics also caused an imbalance in gut bacteria, creating a chain reaction where damaged gut cells release particles that further weaken the intestinal barrier and change the microbiome.

2025 Nature Communications 34 citations
Article Tier 2

Biological effects of polystyrene micro- and nano-plastics on human intestinal organoid-derived epithelial tissue models without and with M cells.

Researchers exposed human intestinal organoid-derived epithelial tissue models with and without M cells to polystyrene micro- and nano-plastics, finding that nano-plastics caused greater disruption of barrier integrity and uptake than micro-plastics, and that M cell-containing models showed enhanced particle translocation compared to standard epithelial models.

2023 Nanomedicine : nanotechnology, biology, and medicine
Article Tier 2

Polystyrene micro- and nanoplastics in a colitis mouse model – effects on biodistribution, macrophage polarization, and gut microbiome

Researchers exposed colitis mouse models to polystyrene micro- and nanoplastics to test whether MNP exposure worsens inflammatory bowel disease, finding that MNPs altered biodistribution and exacerbated inflammatory responses in animals with pre-existing gut inflammation.

2025
Article Tier 2

Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Polystyrene microparticles induced cytotoxic effects in murine macrophages and intestinal epithelial cells at higher concentrations, triggering cell membrane damage, inflammatory cytokine release, and reduced phagocytic function, with smaller particles generally causing greater harm than larger ones at equivalent mass doses.

2021 Scientific Reports 59 citations
Article Tier 2

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Researchers exposed murine fibroblast and canine kidney epithelial cell lines to polystyrene and polyethylene microplastics at various concentrations for up to 24 hours. They observed dose-dependent decreases in cell viability along with dysregulation of inflammatory cytokines and oxidative stress markers. The study suggests that microplastic exposure can trigger inflammatory and oxidative stress responses in mammalian cells at the cellular level.

2021 Exposure and Health 84 citations
Article Tier 2

Polystyrene nanoplastics of different particle sizes regulate the polarization of pro-inflammatory macrophages

Researchers exposed immune cells called macrophages to polystyrene nanoplastics of two different sizes (50 nm and 500 nm) and found that both sizes pushed the cells toward a pro-inflammatory state at higher concentrations. This means the immune cells shifted toward producing inflammation signals rather than healing signals after nanoplastic exposure. Since macrophages are a key defense in the gut, this inflammatory response could help explain how microplastics contribute to intestinal inflammation.

2024 Scientific Reports 35 citations
Article Tier 2

Microplastic Toxicity on Gut Microbiota and Intestinal Cells: Evidence from the Simulator of the Human Intestinal Microbial Ecosystem (SHIME)

Researchers used a simulator of the human intestinal ecosystem to study how polystyrene microplastics affect gut bacteria and intestinal cells. They found that microplastic exposure caused region-specific shifts in gut microbial diversity, altered the ratio of key bacterial groups, and reduced beneficial bacteria. When gut fluids from the simulator were applied to intestinal cell models, they caused increased oxidative stress and mitochondrial dysfunction.

2025 Toxics 1 citations
Article Tier 2

Acute and Sub-Chronic Effects of Microplastics (3 and 10 µm) on the Human Intestinal Cells HT-29

Researchers tested the effects of polystyrene microplastics on human intestinal cells over both short-term and extended 48-day exposure periods, simulating real-world chronic exposure. They found that smaller 3-micrometer particles caused more pronounced damage to cell membranes, while larger 10-micrometer particles generated more oxidative stress. The study suggests that prolonged microplastic exposure could contribute to intestinal health issues due to cumulative cell damage over time.

2021 International Journal of Environmental Research and Public Health 104 citations