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Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Scientific Reports 2021 59 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Matthias Völkl, Julia Rudolph, Julia Rudolph, Matthias Völkl, Matthias Völkl, Matthias Völkl, Matthias Völkl, Valérie Jérôme, Thomas Scheibel Matthias Völkl, Matthias Völkl, Matthias Völkl, Valérie Jérôme, Matthias Völkl, Matthias Völkl, Matthias Völkl, Matthias Völkl, Valérie Jérôme, Thomas Scheibel Thomas Scheibel Valérie Jérôme, Matthias Völkl, Valérie Jérôme, Valérie Jérôme, Valérie Jérôme, Valérie Jérôme, Thomas Scheibel Valérie Jérôme, Valérie Jérôme, Ruth Freitag, Ruth Freitag, Valérie Jérôme, Ruth Freitag, Thomas Scheibel Valérie Jérôme, Valérie Jérôme, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Thomas Scheibel Thomas Scheibel Thomas Scheibel Thomas Scheibel Thomas Scheibel Thomas Scheibel Thomas Scheibel Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Valérie Jérôme, Thomas Scheibel Thomas Scheibel Thomas Scheibel Thomas Scheibel Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Ruth Freitag, Thomas Scheibel Thomas Scheibel

Summary

Polystyrene microparticles induced cytotoxic effects in murine macrophages and intestinal epithelial cells at higher concentrations, triggering cell membrane damage, inflammatory cytokine release, and reduced phagocytic function, with smaller particles generally causing greater harm than larger ones at equivalent mass doses.

Polymers

Body Systems

Immune

Models

Mouse

Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2-6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.

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