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61,005 resultsShowing papers similar to Flow cytometry as new promising detection tool for micro and submicron plastic particles
ClearFlow cytometry as new promising detection tool for micro and submicron plastic particles
Researchers evaluated flow cytometry as a tool for detecting and counting micro- and submicron plastic particles in environmental and biological samples. The method offered rapid throughput and the ability to distinguish plastic particles from biological material, but required careful optimization for complex matrices.
Can flow cytometry emerge as a high-throughput technique for micro- and nanoplastics analysis in complex environmental aqueous matrices?
Researchers reviewed the potential of flow cytometry — a technique that rapidly analyzes individual particles — as a high-throughput tool for detecting micro- and nanoplastics in water samples, finding it excels at measuring particles smaller than 20 micrometers that other methods struggle to detect. Using fluorescent dyes to tag plastics, the approach could enable near-real-time environmental monitoring at a scale no other current technique can match.
Preliminary Results From Detection of Microplastics in Liquid Samples Using Flow Cytometry
Researchers developed a novel flow cytometry approach for in-situ detection and quantification of microplastics in liquid samples using fluorescent staining, testing nine polymer types under controlled laboratory conditions. The method offers a high-throughput alternative to traditional time-consuming microplastic detection protocols that risk sample contamination.
A novel high-throughput analytical method to quantify microplastics in water by flow cytometry
Researchers developed a faster, high-throughput method using flow cytometry — a technology that rapidly counts and characterizes particles in liquid — to measure microplastics in water, achieving about 97% accuracy across multiple plastic types and sizes and offering a practical alternative to slow, labor-intensive microscopy-based counting.
Flow Cytometry as a Rapid Alternative to Quantify Small Microplastics in Environmental Water Samples
Researchers developed a flow cytometry method using fluorescent staining to rapidly detect and quantify small microplastics (1-50 micrometers) in environmental water samples, achieving over 80% recovery rates and significantly reducing analysis time compared to traditional microscopy.
Novel methodology for identification and quantification of microplastics in biological samples
Researchers validated a protocol for identifying and quantifying polyethylene microplastics in biological samples, finding that membrane filtration caused particle retention problems and that flow cytometry offered a more reliable alternative for analysis of biological digests.
Rapid detection of nanoplastics and small microplastics by Nile-Red staining and flow cytometry
Researchers developed a rapid method for detecting nanoplastics and small microplastics by combining Nile-Red fluorescent staining with flow cytometry. The technique can quantify plastic particles in the 0.6 to 15 micrometer range in just 90 seconds, which is hundreds of times faster than conventional spectroscopic methods. The approach showed high detection efficiency for polyethylene, polyvinylchloride, and polystyrene, offering a practical tool for environmental nanoplastic monitoring.
Fast-screening flow cytometry method for detecting nanoplastics in human peripheral blood
Researchers developed a flow cytometry method using a fluorescent dye to detect and measure nanoplastics — plastic particles smaller than 1 micrometer — directly in human blood samples, providing one of the first approaches capable of tracking nanoplastic absorption in people and opening new avenues for studying their health effects.
Flow cytometry as a tool for the rapid enumeration of 1-μm microplastics spiked in wastewater and activated sludge after coagulation-flocculation-sedimentation
Researchers used flow cytometry to rapidly count one-micrometer microplastic particles spiked into wastewater and activated sludge after coagulation-flocculation-sedimentation treatment. They found that aluminum salt-based coagulation removed a significant portion of these very small particles, though removal rates varied depending on the water matrix. The study demonstrates that flow cytometry can be a fast and reliable tool for quantifying micro-nanoplastics in complex wastewater samples.
Quantitively Analyzing the Variation of Micrometer-Sized Microplastic during Water Treatment with the Flow Cytometry-Fluorescent Beads Method
Researchers developed a flow cytometry-fluorescent bead method for quantitatively measuring the removal of micrometer-sized microplastics during water treatment processes, demonstrating a rapid and reliable analytical approach for evaluating treatment plant efficiency.
Fast-screening flow cytometry method for detecting nanoplastics in human peripheral blood
Researchers developed a fast-screening flow cytometry method for detecting nanoplastics in human peripheral blood, addressing the previously uncharacterized question of nanoplastic absorption in human subjects. The method enables detection of polydisperse nanoplastic particles ranging from 1 to 1000 nm in blood samples, providing a clinical screening tool to investigate human exposure to plastic particles and their potential effects on xenobiotic metabolism, nutrient absorption, and cytotoxicity.
Evaluating theEfficiency of Enhanced Coagulationfor Nanoplastics Removal Using Flow Cytometry
Researchers evaluated the efficiency of enhanced coagulation for removing nanoplastics from water using flow cytometry as a quantification tool, addressing the interconnected challenges of nanoplastic removal and detection in conventional water treatment systems.
Separation and flow cytometry analysis of microplastics and nanoplastics
Researchers improved a flow cytometry method for counting and separating microplastics and nanoplastics stained with a fluorescent dye called Nile Red. By adjusting the chemical solution used in detection, they reduced particle clumping and improved measurement accuracy for plastic particles across a range of sizes. The refined technique offers a faster and more reliable way to quantify plastic pollution in environmental and biological samples.
Differentiating Microplastics from Natural Particles in Aqueous Suspensions Using Flow Cytometry with Machine Learning
Researchers developed a stain-free flow cytometry method combined with machine learning to rapidly distinguish microplastics from natural particles like algae and sediment in water samples. The approach achieved identification accuracies over 93% and was validated in freshwater environmental samples, offering a time-efficient screening tool for microplastic monitoring.
Microplastics and nanoplastics detection using flow cytometry: Challenges and methodological advances with fluorescent dye application
This review examined the use of flow cytometry for detecting and counting micro- and nanoplastics in water, including challenges with fluorescent dye application. Researchers found that the technique can detect particles as small as 200 nanometers but that undissolved dye in water samples remains a significant source of measurement error. The review highlights recent methodological improvements and identifies remaining challenges that need to be addressed for reliable nanoplastic quantification.
A strategy for quantifying microplastic particles in membrane filtration processes using flow cytometry
Researchers demonstrated that flow cytometry can rapidly quantify plastic microbead concentrations (1-5 µm) in water with or without added humic acid, and applied the method to evaluate two microfiltration membranes. A 0.45 µm membrane achieved over 99% rejection while a 5 µm membrane showed variable rejection (40-95%), with the technique also revealing a 33% reduction in median particle size permeating through the larger membrane in humic acid conditions.
Plastibodies for multiplexed detection and sorting of microplastic particles in high-throughput
Researchers developed a high-throughput flow cytometry method using material-binding peptide antibodies (plastibodies) for multiplexed detection and sorting of microplastic particles, enabling sensitive and rapid quantification in aqueous samples.
A Droplet-Based Microfluidic Impedance Flow Cytometer for Detection of Micropollutants in Water
A droplet-based microfluidic impedance cytometer was designed and tested for in-situ detection of microplastic particles in water, offering a portable and rapid alternative to laboratory-based analytical methods.
An effective solution to simultaneously analyze size, mass and number concentration of polydisperse nanoplastics in a biological matrix: asymmetrical flow field fractionation coupled with a diode array detector and multiangle light scattering
Researchers developed an asymmetrical flow field-flow fractionation method coupled with a diode array detector and multiangle light scattering to simultaneously measure the size, mass, and number concentration of polydisperse nanoplastics in biological matrices, providing a more accurate tool for assessing nanoplastic pollution levels.
Multidimensional analysis methods for flow cytometry : Pushing the boundaries
This thesis developed new methods for analyzing multidimensional flow cytometry data to better identify cell populations. While a bioinformatics and immunology paper, flow cytometry is also used in cutting-edge research to detect and quantify micro- and nanoplastics in biological fluids.
Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry
Scientists developed an improved method for staining microplastics with a fluorescent dye (Nile red) that embeds the dye inside the plastic particles rather than just coating the surface, resulting in much brighter and more reliable detection. Combined with high-speed flow cytometry, the technique can rapidly count microplastic particles smaller than 10 µm in environmental water samples with recovery rates above 99%. Faster and more accurate counting methods like this are important for scaling up microplastic monitoring across many water sources.
Rapid methods for the quantification of ingested nano-and microplastics in marine fish by imaging flow cytometry
Researchers developed a rapid, high-throughput method using imaging flow cytometry to quantify nano- and microplastics ingested by marine fish. The optimized technique uses Nile Red fluorescent staining and morphology-based corrections to accurately count plastic particles, providing a faster and more reliable alternative to conventional detection methods for ecological risk assessments.
Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals
Imaging flow cytometry was adapted to study how aquatic animal cells take up microplastic particles, enabling detailed, high-throughput analysis of cellular responses to plastic ingestion. This method could help researchers better understand how microplastics harm marine and freshwater organisms at the cellular level.
Evaluating the Efficiency of Enhanced Coagulation for Nanoplastics Removal Using Flow Cytometry
Flow cytometry was used to quantify fluorescently labeled nanoplastics removal during enhanced coagulation-flocculation water treatment, demonstrating that this technique enables accurate detection and process optimization for nanoplastic removal in drinking water treatment.