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Flow cytometry as a tool for the rapid enumeration of 1-μm microplastics spiked in wastewater and activated sludge after coagulation-flocculation-sedimentation
Summary
Researchers used flow cytometry to rapidly count one-micrometer microplastic particles spiked into wastewater and activated sludge after coagulation-flocculation-sedimentation treatment. They found that aluminum salt-based coagulation removed a significant portion of these very small particles, though removal rates varied depending on the water matrix. The study demonstrates that flow cytometry can be a fast and reliable tool for quantifying micro-nanoplastics in complex wastewater samples.
Considering the limited literature and the difficulty of quantifying 1-μm micro-nanoplastics (1-μm MNP) in complex aqueous matrices such as wastewater and sludge, the removal rate of these very small particles in wastewater treatment plants (WWTP) represents a major challenge. In this study, coagulation-flocculation-sedimentation (CFS) with aluminum salts was investigated to evaluate the removal of 1-μm MNPs spiked in tap water, raw wastewater, pre-settled wastewater, and activated sludge. Quantification of 1-μm MNP was performed using the high-throughput flow cytometry (FCM) analysis which takes only a few minutes and produces results with high accuracy and reproducibly. The results indicated that the 1-μm MNPs were highly stable in pure water and unable to settle rapidly. In raw wastewater, sedimentation without coagulants removed less than 4% of 1-μm MNP. Conversely, CFS treatment showed a significant improvement in the removal of 1-μm MNP from wastewater. At dosages of 0.3-3 mg Al/L, the removal of MNPs in wastewater reached 30% and no flocs were observed, while floc formation was visible with increased dosages of 3-12 mg Al/L, obtaining MNP removal greater than 90%. CFS in activated sludge with a solids content of 5800 mg MLSS/L registered the highest removal efficiency (95-99%) even for dosages of 0.3-60 mg Al/L and pH dropping to 5. However, activated sludge showed extremely high removal efficiency of MNPs (97.3 ± 0.9%) even without coagulants. The large, dense flocs that constitute activated sludge appear particularly efficient in capturing 1-μm MNPs during the sedimentation process even in the absence of coagulants.
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