We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Flow Cytometry as a Rapid Alternative to Quantify Small Microplastics in Environmental Water Samples
AI summary Read the abstract
Researchers developed a flow cytometry method using fluorescent staining to rapidly detect and quantify small microplastics (1-50 micrometers) in environmental water samples, achieving over 80% recovery rates and significantly reducing analysis time compared to traditional microscopy.
The most frequently used method to quantify microplastics (MPs) visually by microscope is time consuming and labour intensive, where the method is also hindered by the size limitation at 10 µm or even higher. A method is proposed to perform pre-concentration of MPs by vacuum filtration, hydrogen peroxide wet digestion, fluorescent staining and flow cytometric determination to rapidly detect and quantify small MPs sized from 1–50 µm. The method performance was evaluated by the spiking of seven different types of polymer, including polystyrene (PS), low-density polyethylene (LDPE), polypropylene (PP), poly(methyl methacrylate) (PMMA), polyvinyl chloride (PVC), polylactic acid (PLA) and acrylonitrile butadiene styrene (ABS) at different levels (400, 4000, 40,000 particles mL−1), with a satisfactory overall % recoveries (101 ± 19.4%) observed, where in general no significant difference between the two methods was observed. Furthermore, a pre-concentration process by vacuum filtration was introduced to reduce the matrix effect. After pre-concentration, satisfactory % recoveries and accuracy in MP counts resulted from both ultrapure water (94.33 ± 11.16%) and sea water (103.17 ± 9.50%) samples. The validated method using flow cytometry can be used to quantify MPs in environmental water samples that can reduce time and human resources.
More Papers Like This
A novel high-throughput analytical method to quantify microplastics in water by flow cytometry
AI summary Read the abstract
Researchers developed a faster, high-throughput method using flow cytometry — a technology that rapidly counts and characterizes particles in liquid — to measure microplastics in water, achieving about 97% accuracy across multiple plastic types and sizes and offering a practical alternative to slow, labor-intensive microscopy-based counting.
Preliminary Results From Detection of Microplastics in Liquid Samples Using Flow Cytometry
AI summary Read the abstract
Researchers developed a novel flow cytometry approach for in-situ detection and quantification of microplastics in liquid samples using fluorescent staining, testing nine polymer types under controlled laboratory conditions. The method offers a high-throughput alternative to traditional time-consuming microplastic detection protocols that risk sample contamination.
Can flow cytometry emerge as a high-throughput technique for micro- and nanoplastics analysis in complex environmental aqueous matrices?
AI summary Read the abstract
Researchers reviewed the potential of flow cytometry — a technique that rapidly analyzes individual particles — as a high-throughput tool for detecting micro- and nanoplastics in water samples, finding it excels at measuring particles smaller than 20 micrometers that other methods struggle to detect. Using fluorescent dyes to tag plastics, the approach could enable near-real-time environmental monitoring at a scale no other current technique can match.
Quantitively Analyzing the Variation of Micrometer-Sized Microplastic during Water Treatment with the Flow Cytometry-Fluorescent Beads Method
AI summary Read the abstract
Researchers developed a flow cytometry-fluorescent bead method for quantitatively measuring the removal of micrometer-sized microplastics during water treatment processes, demonstrating a rapid and reliable analytical approach for evaluating treatment plant efficiency.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
AI summary Read the abstract
Researchers evaluated flow cytometry as a detection tool for micro- and nanoplastics, testing its ability to rapidly identify and count plastic particles in environmental and biological samples. Results demonstrated that flow cytometry offers a promising high-throughput approach for microplastic detection compared to more time-intensive conventional methods.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.