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Papers
20 resultsShowing papers similar to A novel high-throughput analytical method to quantify microplastics in water by flow cytometry
ClearFlow Cytometry as a Rapid Alternative to Quantify Small Microplastics in Environmental Water Samples
Researchers developed a flow cytometry method using fluorescent staining to rapidly detect and quantify small microplastics (1-50 micrometers) in environmental water samples, achieving over 80% recovery rates and significantly reducing analysis time compared to traditional microscopy.
Can flow cytometry emerge as a high-throughput technique for micro- and nanoplastics analysis in complex environmental aqueous matrices?
Researchers reviewed the potential of flow cytometry — a technique that rapidly analyzes individual particles — as a high-throughput tool for detecting micro- and nanoplastics in water samples, finding it excels at measuring particles smaller than 20 micrometers that other methods struggle to detect. Using fluorescent dyes to tag plastics, the approach could enable near-real-time environmental monitoring at a scale no other current technique can match.
Quantitively Analyzing the Variation of Micrometer-Sized Microplastic during Water Treatment with the Flow Cytometry-Fluorescent Beads Method
Researchers developed a flow cytometry-fluorescent bead method for quantitatively measuring the removal of micrometer-sized microplastics during water treatment processes, demonstrating a rapid and reliable analytical approach for evaluating treatment plant efficiency.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
Researchers evaluated flow cytometry as a detection tool for micro- and nanoplastics, testing its ability to rapidly identify and count plastic particles in environmental and biological samples. Results demonstrated that flow cytometry offers a promising high-throughput approach for microplastic detection compared to more time-intensive conventional methods.
Preliminary Results From Detection of Microplastics in Liquid Samples Using Flow Cytometry
Researchers developed a novel flow cytometry approach for in-situ detection and quantification of microplastics in liquid samples using fluorescent staining, testing nine polymer types under controlled laboratory conditions. The method offers a high-throughput alternative to traditional time-consuming microplastic detection protocols that risk sample contamination.
Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry
Scientists developed an improved method for staining microplastics with a fluorescent dye (Nile red) that embeds the dye inside the plastic particles rather than just coating the surface, resulting in much brighter and more reliable detection. Combined with high-speed flow cytometry, the technique can rapidly count microplastic particles smaller than 10 µm in environmental water samples with recovery rates above 99%. Faster and more accurate counting methods like this are important for scaling up microplastic monitoring across many water sources.
Differentiating Microplastics from Natural Particles in Aqueous Suspensions Using Flow Cytometry with Machine Learning
Researchers developed a stain-free flow cytometry method combined with machine learning to rapidly distinguish microplastics from natural particles like algae and sediment in water samples. The approach achieved identification accuracies over 93% and was validated in freshwater environmental samples, offering a time-efficient screening tool for microplastic monitoring.
Micro-flow imaging for in-situ and real-time enumeration and identification of microplastics in water
Researchers tested micro-flow imaging (MFI) — a technology that uses high-speed cameras to photograph particles flowing through liquid — as a faster, more consistent way to count and characterise microplastics in water samples. The method automatically captures size, shape, colour, and transparency in real time with minimal sample preparation, making it a promising tool for field monitoring of waterways where current techniques are slow and labour-intensive.
Evaluating theEfficiency of Enhanced Coagulationfor Nanoplastics Removal Using Flow Cytometry
Researchers evaluated the efficiency of enhanced coagulation for removing nanoplastics from water using flow cytometry as a quantification tool, addressing the interconnected challenges of nanoplastic removal and detection in conventional water treatment systems.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
Researchers evaluated flow cytometry as a tool for detecting and counting micro- and submicron plastic particles in environmental and biological samples. The method offered rapid throughput and the ability to distinguish plastic particles from biological material, but required careful optimization for complex matrices.
Flow cytometry as a tool for the rapid enumeration of 1-μm microplastics spiked in wastewater and activated sludge after coagulation-flocculation-sedimentation
Researchers used flow cytometry to rapidly count one-micrometer microplastic particles spiked into wastewater and activated sludge after coagulation-flocculation-sedimentation treatment. They found that aluminum salt-based coagulation removed a significant portion of these very small particles, though removal rates varied depending on the water matrix. The study demonstrates that flow cytometry can be a fast and reliable tool for quantifying micro-nanoplastics in complex wastewater samples.
Real-Time Quantification of Microplastics in Aquatic Systems via Fluorescence Microscopy
Researchers developed a real-time fluorescence microscopy method capable of quantifying microplastics in aquatic systems with high precision, providing a faster and more accessible tool for monitoring microplastic contamination in drinking water reservoirs.
Quantitative Detection of Microplastics in Water through Fluorescence Signal Analysis
Researchers developed an automatic, portable fluorescence-based system for quantitative detection of microplastics in water, using dye-stained particles flowing through a laser beam to enable fast and objective counting without manual microscopy.
Novel methodology for identification and quantification of microplastics in biological samples
Researchers validated a protocol for identifying and quantifying polyethylene microplastics in biological samples, finding that membrane filtration caused particle retention problems and that flow cytometry offered a more reliable alternative for analysis of biological digests.
Using optimized particle imaging of micro-Raman to characterize microplastics in water samples
Researchers developed a micro-Raman automatic particle identification technique that can characterize microplastics in water samples up to 100 times faster than traditional point-by-point detection methods, while maintaining high precision for identifying polymer types, sizes, and morphologies.
Microplastics’ Shape and Morphology Analysis in the Presence of Natural Organic Matter Using Flow Imaging Microscopy
Researchers introduced an innovative flow imaging microscopy approach for rapidly identifying and quantifying microplastics in wastewater treatment plant samples. The study demonstrates that this method can simultaneously capture and classify polyethylene and polystyrene particles while also analyzing how natural organic matter affects microplastic shape and morphology.
Separation and flow cytometry analysis of microplastics and nanoplastics
Researchers improved a flow cytometry method for counting and separating microplastics and nanoplastics stained with a fluorescent dye called Nile Red. By adjusting the chemical solution used in detection, they reduced particle clumping and improved measurement accuracy for plastic particles across a range of sizes. The refined technique offers a faster and more reliable way to quantify plastic pollution in environmental and biological samples.
Rapid and reliable detection of microplastics in drinking water using fluorescence microscopy
Researchers developed a fluorescence-based method for rapid detection and quantification of microplastics in drinking water, addressing the need for faster and more practical monitoring tools. The method achieved high sensitivity and allowed polymer discrimination without requiring expensive spectroscopic instrumentation.
The power of a multi-technique approach for the reliable quantification of microplastics in water
Researchers applied a multi-technique analytical approach combining several spectroscopic and microscopic methods to improve the reliability of microplastic quantification in environmental samples. The combined approach reduced false positives and improved polymer identification accuracy compared to any single method used alone.
Microplastics and nanoplastics detection using flow cytometry: Challenges and methodological advances with fluorescent dye application
This review examined the use of flow cytometry for detecting and counting micro- and nanoplastics in water, including challenges with fluorescent dye application. Researchers found that the technique can detect particles as small as 200 nanometers but that undissolved dye in water samples remains a significant source of measurement error. The review highlights recent methodological improvements and identifies remaining challenges that need to be addressed for reliable nanoplastic quantification.