Papers

61,005 results
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Article Tier 2

Visualizing and assessing the size-dependent oral uptake, tissue distribution, and detrimental effect of polystyrene microplastics in Eisenia fetida

Researchers investigated size-dependent effects of polystyrene microplastics on earthworms (Eisenia fetida) using particles of 70 nanometers, 1 micrometer, and 10 micrometers at various doses. They found that smaller particles were more readily taken up into tissues and caused greater oxidative stress and tissue damage. The study suggests that nanoscale plastic particles may pose higher ecological risks to soil organisms than larger microplastics due to their enhanced ability to penetrate biological barriers.

2022 Environmental Pollution 40 citations
Article Tier 2

Earthworms ingest microplastic fibres and nanoplastics with effects on egestion rate and long-term retention

Researchers used specially labeled microplastic fibers and nanoplastics to track their uptake and retention in earthworms. They found that earthworms ingested both types of particles, but nanoplastics were retained in body tissues for much longer than fibers, which were mostly excreted within days. The study reveals that soil organisms can accumulate very small plastic particles over time, with potential implications for soil food webs.

2021 The Science of The Total Environment 133 citations
Article Tier 2

Ecotoxicological effects of different size ranges of industrial-grade polyethylene and polypropylene microplastics on earthworms Eisenia fetida

Researchers exposed earthworms to industrial-grade polyethylene and polypropylene microplastics of various sizes and found that the worms ingested all types of particles tested. The microplastics caused oxidative stress and DNA damage in the earthworms, with the severity depending on both the size and type of plastic. Gene analysis revealed that exposure disrupted pathways related to nervous system function, oxidative stress, and inflammation, indicating that microplastics pose ecological risks to important soil organisms.

2021 The Science of The Total Environment 128 citations
Article Tier 2

Size effects of microplastics on accumulation and elimination of phenanthrene in earthworms

Researchers examined how microplastic particle size affects the accumulation and elimination of the pollutant phenanthrene in earthworms. They found that smaller microplastics enhanced phenanthrene uptake in earthworm tissues, while larger particles had a less pronounced effect. The study demonstrates that microplastic size influences how organic pollutants interact with soil organisms, with finer particles potentially increasing contaminant bioavailability.

2020 Journal of Hazardous Materials 138 citations
Article Tier 2

Impact of Conventional vs. Biodegradable and Compostable Microplastics on Eisenia fetida S.: An Ecopathological Approach

Researchers compared the effects of biodegradable and conventional polyethylene microplastics on soil-dwelling earthworms and found that both types caused increased mortality, decreased biomass, and tissue damage after 14 days of exposure. The study suggests that biodegradable microplastics are not necessarily safer than conventional ones, and that detailed tissue analysis can reveal harmful sublethal effects not captured by standard toxicity tests.

2026 Environmental Toxicology
Article Tier 2

Earthworms Exposed to Polyethylene and Biodegradable Microplastics in Soil: Microplastic Characterization and Microbial Community Analysis

Researchers exposed earthworms to biodegradable and conventional polyethylene microplastics in natural soil and found that worms ingested both types. The biodegradable plastic showed signs of partial breakdown in the earthworm gut, while conventional polyethylene remained unchanged. Although microplastics did not significantly alter the soil or gut microbiome in this study, the results confirm that earthworms transport microplastics through soil ecosystems.

2023 ACS Agricultural Science & Technology 42 citations
Article Tier 2

Size-dependent vector effects of microplastics on bioaccumulation of hydrophobic organic contaminants in earthworm: A dual-dosing study

Researchers developed a dual-dosing method to directly measure how microplastics act as carriers for hydrophobic organic contaminants in earthworms. The study found that smaller microplastic particles had greater vector effects, increasing bioaccumulation of pollutants, and that dermal uptake played a significant role in contaminant transfer from microplastics to organisms.

2024 Environment International 11 citations
Article Tier 2

Microplastic cytotoxicity and the phagocytic response of earthworm immune cells

Researchers tested the effects of polyethylene microplastics on earthworm immune cells in laboratory conditions and found that cells engulfed 85% of small particles (1-10 micrometers) but showed negligible uptake of larger ones (20-27 micrometers). Both particle sizes caused dramatic drops in cell viability to just 6-7%, compared to 94% in untreated controls. The findings reveal that different microplastic sizes trigger distinct pathways of cellular damage in soil organisms.

2025 Environmental Science and Pollution Research 1 citations
Article Tier 2

Microplastics as a Vector for HOC Bioaccumulation in Earthworm Eisenia fetida in Soil: Importance of Chemical Diffusion and Particle Size

Researchers investigated whether microplastics act as vectors for hydrophobic organic contaminant (HOC) bioaccumulation in earthworms, testing two scenarios with polyethylene particles of different sizes and polychlorinated biphenyls in soil. Results showed that clean microplastics in contaminated soil reduced HOC bioaccumulation, while smaller precontaminated microplastics enhanced transfer of PCBs to earthworms, demonstrating that particle size and prior contamination status are critical factors.

2020 Environmental Science & Technology 90 citations
Article Tier 2

A comparison of the toxicity induced by the exposure to microplastics made of a conventional and a biodegradable polymer on the earthworm Eisenia fetida

Researchers compared the toxicity of conventional versus biodegradable polymer microplastics on the earthworm Eisenia fetida, evaluating whether biodegradable alternatives present reduced ecotoxicological risk in soil environments where microplastic contamination is increasingly documented.

2022 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions

Researchers compared the effects of new versus sunlight-aged polyethylene microplastics on earthworms at real-world contamination levels, finding that aged particles caused more harm. The aged microplastics accumulated more in earthworm guts, caused greater tissue damage, and shifted gut bacteria away from beneficial species toward harmful ones. This is significant because most microplastics in the environment have been aged by sunlight, meaning their actual impact on soil health and the food chain may be worse than lab studies using fresh plastics suggest.

2023 Environmental Science & Technology 55 citations
Article Tier 2

Size effects of polystyrene microplastics on the accumulation and toxicity of (semi-)metals in earthworms

Researchers studied how different sizes of polystyrene microplastics and nanoplastics affect the uptake of cadmium and arsenic in earthworms. They found that microplastics facilitated greater accumulation of these metals than nanoplastics by damaging intestinal integrity, with proteomic and metabolomic analysis revealing disruptions to the earthworms' immune and metabolic systems.

2021 Environmental Pollution 51 citations
Article Tier 2

Exposure to mm-scale microplastic particles does not cause weight loss in two earthworm species belonging to different ecological groups

Researchers tested whether relatively large microplastic fragments (1-5 mm) from polyethylene film cause weight loss in two common earthworm species by diluting their food supply. Contrary to their expectations, the microplastics did not reduce earthworm body weight even in food-limited conditions, despite both species ingesting the particles. The findings challenge the widely cited food dilution hypothesis and suggest that the effects of larger microplastics on soil organisms may be less straightforward than assumed.

2025 Environmental Science and Pollution Research 3 citations
Article Tier 2

Adverse effects of microplastics on earthworms: A critical review

This critical review of 65 publications summarized the adverse effects of microplastics on earthworms, finding impacts on growth, behavior, oxidative stress, gene expression, and gut microbiota, with particle size, concentration, and co-occurring pollutants influencing toxicity outcomes.

2022 The Science of The Total Environment 111 citations
Article Tier 2

Effects of polyethylene microplastics stress on soil physicochemical properties mediated by earthworm Eisenia fetida

Researchers exposed earthworms to polyethylene microplastics of two sizes and found that smaller particles (13 micrometers) were more toxic than larger ones (130 micrometers), reducing survival and growth more severely. The microplastics caused oxidative stress in the worms and altered key soil properties including pH and organic carbon content. Since earthworms play a vital role in maintaining healthy soil for agriculture, this damage could affect soil quality and ultimately the food grown in microplastic-contaminated farmland.

2024 Environmental Science and Pollution Research 14 citations
Article Tier 2

Combined ecotoxicological effects of different-sized polyethylene microplastics and imidacloprid on the earthworms (Eisenia fetida)

Researchers exposed earthworms to different sizes of polyethylene microplastics combined with the pesticide imidacloprid and found that particle size significantly influenced the combined toxic effects. Smaller microplastics tended to increase pesticide toxicity more than larger ones, affecting earthworm survival, growth, and antioxidant responses. The findings highlight how microplastic size matters when assessing the joint environmental risks of plastic pollution and agricultural chemicals in soil.

2023 The Science of The Total Environment 56 citations
Article Tier 2

Defense responses in earthworms (Eisenia fetida) exposed to low-density polyethylene microplastics in soils

Researchers exposed earthworms to low-density polyethylene microplastics in soil at various concentrations for 28 days. They found that the earthworms ingested microplastics in a dose-dependent manner and actually broke some particles into smaller pieces during digestion, with about 30% more particles under 100 micrometers found in their excrement compared to the original soil. At higher concentrations, the microplastics triggered oxidative stress and neurotoxic responses, suggesting potential ecological risks to soil organisms.

2019 Ecotoxicology and Environmental Safety 261 citations
Article Tier 2

Toxicological effects of polystyrene microplastics on earthworm (Eisenia fetida)

Researchers exposed earthworms to two sizes of polystyrene microplastics in soil for 14 days and found evidence of intestinal cell damage, oxidative stress, and DNA damage. The larger particles accumulated more in earthworm intestines, while both sizes triggered changes in key antioxidant markers. The study demonstrates that microplastic contamination in soil can cause measurable biological harm to important soil organisms.

2019 Environmental Pollution 383 citations
Article Tier 2

An Evaluation of the Quantitative Concentration of Microplastic in Dendrobaena veneta and Lumbricus terrestris Tissues from Laboratory and Environmental Cultures

Researchers exposed two earthworm species (Dendrobaena veneta and Lumbricus terrestris) to mixed microplastics at different concentrations in laboratory and environmental cultures and measured MP accumulation in tissues using FTIR and fluorescence. Both species accumulated MPs in tissues with significant polymer content confirmed, and digestive enzyme activity caused observable structural changes to the plastic particles.

2025 Applied Sciences
Article Tier 2

Earthworms on a microplastics diet

Researchers found that environmentally relevant concentrations of polyethylene microplastics added to plant litter on soil surfaces led to reduced growth and elevated mortality in the earthworm Lumbricus terrestris, and that earthworms may themselves transport ingested microplastics deeper into soils.

2016 Science 4 citations
Article Tier 2

Ecotoxicological effects of micronized car tire wear particles and their heavy metals on the earthworm (Eisenia fetida) in soil

Researchers exposed earthworms to microplastics from ground-up car tires at various concentrations and sizes over 14 and 28 days. They found that the worms preferentially consumed smaller particles, which led to increased heavy metal accumulation and significant signs of oxidative stress at higher concentrations. The study highlights that tire-derived microplastics in soil can harm terrestrial organisms through both physical ingestion and the release of associated toxic metals.

2021 The Science of The Total Environment 100 citations
Article Tier 2

Reproduction, metabolic enzyme activity, and metabolomics in earthworms Eisenia fetida exposed to different polymer microplastics

Researchers exposed earthworms to microplastics from three different polymer types, including both conventional and biodegradable plastics, at environmentally relevant concentrations. They found that polypropylene microplastics had the most pronounced effects on reproduction and metabolic enzyme activity, while biodegradable plastics also disrupted earthworm metabolism. The study demonstrates that different plastic polymers pose varying levels of risk to soil-dwelling organisms.

2025 Ecotoxicology and Environmental Safety 2 citations
Article Tier 2

Microplastic-Earthworm Interactions: A Critical Review

This critical review examines how microplastics from diverse plastic waste categories accumulate in terrestrial and aquatic ecosystems and interact with earthworms, a key soil organism. The authors synthesize evidence on the deleterious effects of increasing microplastic concentrations on soil properties, microbiota, and earthworm physiology.

2024 International Journal of Ecology and Environmental Sciences 1 citations
Article Tier 2

Insight into Bioaccumulation of Decabromodiphenyl Ethane in Eisenia fetida Increased by Microplastics

Researchers found that microplastics made from electronics casings significantly increased the accumulation of a brominated flame retardant chemical in earthworms over time. While the microplastics initially slowed absorption of the chemical, after 28 days they promoted greater bioaccumulation by altering the soil environment and the earthworms' gut bacteria. The study suggests that the co-occurrence of electronic waste microplastics and flame retardants in soil may amplify chemical exposure in soil organisms.

2023 Environmental Science & Technology 23 citations