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61,005 resultsShowing papers similar to PhotothermalInfrared Imaging of Nanoplastics in HumanCells with Nanoscale Resolution
ClearPhotothermal Infrared Imaging of Nanoplastics in Human Cells with Nanoscale Resolution
Researchers demonstrated a new photothermal infrared imaging technique capable of detecting and localizing nanoplastics inside individual human cells at nanoscale resolution. The study found that polystyrene nanoparticles accumulated around cell nuclei, and that this advanced imaging approach overcomes the spatial resolution limitations of conventional infrared spectroscopy for tracking nanoplastics in biological tissues.
Detection ofUnlabeled Polystyrene Micro- and Nanoplasticsin Mammalian Tissue by Optical Photothermal Infrared Spectroscopy
Researchers evaluated optical photothermal infrared (O-PTIR) spectroscopy for detecting unlabeled polystyrene micro- and nanoplastics down to 200 nm in mammalian kidney tissues and 3D cell cultures. O-PTIR outperformed conventional FTIR in spatial resolution and signal-to-noise ratio, and machine learning accelerated particle detection with minimal human error.
Label-free nano- and microplastics detection in mammalian tissue by photothermal infrared spectroscopy
Researchers applied optical photothermal infrared spectroscopy to detect and identify nano- and microplastics smaller than 1 µm in mammalian tissue sections without requiring labels or lengthy digestion protocols. The method successfully localized polystyrene particles in tissue samples with chemical specificity, offering a faster workflow for nanoplastic detection in biological matrices.
Detection of Unlabeled Micro- and Nanoplastics in Unstained Tissue with Optical Photothermal Infrared Spectroscopy
Researchers demonstrated that optical photothermal infrared spectroscopy can detect unlabeled micro- and nanoplastics as small as 250 nanometers in mammalian tissue samples without staining or labeling. The technique significantly outperformed traditional FTIR spectroscopy in spatial resolution and signal quality when imaging particles in complex biological matrices. The study also introduced a semi-automated machine learning analysis to speed up detection, offering a powerful new tool for studying nanoplastic accumulation in tissues.
Label-Free Identification and Imaging of Microplastic and Nanoplastic Biouptake Using Optical Photothermal Infrared Microspectroscopy
Researchers developed a new imaging technique that can locate and identify microplastic and nanoplastic particles inside whole organisms without needing fluorescent labels. Using a method called optical photothermal infrared microscopy, they tracked polystyrene particles as small as 1 micrometer in roundworms. This tool could help scientists better understand how plastic particles are taken up by living things and where they accumulate in the body.
Detection of Unlabeled Polystyrene Micro- and Nanoplastics in Mammalian Tissue by Optical Photothermal Infrared Spectroscopy
Researchers demonstrated that a new imaging technique called O-PTIR spectroscopy can detect unlabeled plastic particles as small as 200 nanometers inside mammalian tissues without damaging the samples. Combined with machine learning for faster analysis, this method significantly outperforms traditional infrared spectroscopy for finding nanoplastics in biological tissue. Better detection tools like this are essential for understanding how much plastic actually accumulates in human organs.
Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections
Researchers developed a method using optical photothermal infrared spectroscopy to detect and map microplastics directly within tissue sections without requiring chemical or enzymatic digestion. The study suggests this approach preserves spatial information about where microplastics are located within tissue architecture, overcoming a key limitation of conventional digestion-based methods that can lose some particles.
Vibrational photothermal imaging from single molecules to human subjects (Conference Presentation)
This conference presentation reviews vibrational photothermal microscopy—a label-free technique using infrared pump and visible probe beams—as a tool for imaging chemical bonds in biological systems including bacteria, cells, and organisms. The technique enables real-time chemical mapping without fluorescent labels and has been applied to detect micro- and nanoplastics in biological tissues.
Single-Particle Analysis of the Photodegradation of Submicron Polystyrene Particles Using Infrared Photothermal Heterodyne Imaging.
Researchers used a new infrared imaging technique to observe how submicron polystyrene particles physically and chemically degrade under UV light, finding significant chemical changes within just 6 hours. This is one of the first methods capable of tracking photodegradation of very small plastic particles, improving our understanding of how nanoplastics form and age in the environment.
Photothermal Heterodyne Imaging of Micron Sized Objects
Researchers tested a sensitive imaging technique called photothermal heterodyne imaging to detect and visualize micron-sized polymer beads similar in size to microplastic particles. The method shows promise as a non-destructive way to detect and characterize small plastic particles in various environments.
Characterization of microplastics in tap water by optical photothermal infrared
Researchers used optical photothermal infrared spectroscopy to characterize microplastics in tap water, identifying particles as small as a few micrometers that conventional FTIR techniques cannot resolve. The higher detection sensitivity revealed that microplastic concentrations in drinking water are likely underestimated by standard methods.
Using Infrared Photothermal Heterodyne Imaging to Characterize Micro- and Nanoplastics in Complex Environmental Matrices
Researchers introduced infrared photothermal heterodyne imaging (IR-PHI) as a 300 nm resolution technique for identifying and quantifying micro- and nanoplastics in complex environmental matrices, demonstrating its application to nylon tea bag leachates and sediment samples.
Analysing micro- and nanoplastics with cutting-edge infrared spectroscopy techniques: a critical review
This review evaluates cutting-edge infrared spectroscopy techniques for detecting and analyzing micro- and nanoplastics in environmental and food samples. Better detection methods are crucial for understanding human exposure because they allow scientists to measure smaller particles more accurately, including nanoplastics that are small enough to cross biological barriers and accumulate in human tissues.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Picogram-Level Nanoplastic Analysis with Nanoelectromechanical System Fourier Transform Infrared Spectroscopy: NEMS-FTIR
Researchers developed a NEMS-FTIR approach combining nanoelectromechanical systems with Fourier transform infrared spectroscopy for chemical characterization and quantification of nanoplastics, achieving picogram-level detection limits ranging from 101 to 353 pg for polypropylene, polystyrene, and polyvinyl chloride nanoplastics with diameters from 54 to 262 nm.
Raman-spektroskopische Charakterisierung von Zellen und Gewebe nach Exposition mit Nanoplastik
Researchers exposed human monocytic THP-1 cells, trophoblasts, and placenta cells to primary and secondary nanoplastic particles at 100 particles/cell in sizes of 200 nm and 60 nm, then used confocal laser scanning microscopy and Raman microspectroscopy to locate and characterize intracellular nanoplastics.
Photothermal radiometric image identification of microplastics through near-infrared excitation
Researchers demonstrated near-infrared photothermal radiometric imaging as a rapid tool for identifying and visualizing different microplastic types, using excitation wavelengths in the 1662-1725 nm range specific to polyethylene terephthalate, polystyrene, polyvinyl chloride, and polypropylene. Each plastic type in a 2 mm square area was distinguishable after 20 seconds of irradiation using a commercially available thermal camera.
Contributions of Fourier transform infrared spectroscopy in microplastic pollution research: A review
This review covers advances in Fourier transform infrared (FTIR) spectroscopy techniques — including chemical imaging — for identifying polymer types in microplastic samples and tracing their fate in different environmental matrices.
Chemical characterization of microplastics from biosolids: a comparison of FTIR and O-PTIR microspectroscopy
Researchers compared conventional FTIR microspectroscopy with the emerging Optical Photothermal Infrared (O-PTIR) technique for chemical characterization and polymer-type identification of microplastics extracted from biosolids, finding that O-PTIR's submicron resolution and artifact-free spectra offer advantages over traditional methods.
Optical photothermal infrared spectroscopy with simultaneously acquired Raman spectroscopy for two-dimensional microplastic identification
Researchers demonstrated that optical photothermal infrared spectroscopy combined with simultaneous Raman acquisition enables more reliable two-dimensional microplastic identification, overcoming limitations of individual FTIR or Raman techniques alone.
Characterization of microplastics in tap water by optical photothermal infrared
Researchers characterized microplastics in tap water using optical photothermal infrared spectroscopy, a technique that can identify particles smaller than 10 micrometers with high chemical specificity. The method detected a broader range of particle sizes than conventional FTIR microscopy, revealing higher microplastic concentrations in tap water than previously reported.
A tutorial on optical photothermal infrared (O-PTIR) microscopy
This tutorial reviews optical photothermal infrared microscopy, a technique that achieves up to 30 times better spatial resolution than conventional infrared imaging. Researchers describe how this method enables chemical identification of materials at the sub-micrometer scale, with applications ranging from biomedical research to microplastics detection. The technology is particularly valuable for environmental scientists who need to identify and characterize extremely small plastic particles in complex samples.
Unveiling Hidden Threats: Introduction of a Routine Workflow for Label-Free and Non-destructive Detection of Microplastics in Human FFPE Tissue Sections
Researchers developed a new workflow using mid-infrared photothermal microscopy to detect and identify microplastic particles directly in preserved human colon tissue sections. The method allows non-destructive, label-free identification of polymer types within tissue without special sample preparation. The study introduces a practical approach that could enable routine screening for microplastics in human tissues during standard medical examinations.
IdentifyingMicroplastics in Laboratory and AtmosphericAerosol Mixtures via Optical Photothermal Infrared and Raman Microspectroscopy
This study applied optical photothermal infrared spectroscopy to identify microplastics in atmospheric aerosol mixtures, demonstrating that the technique can distinguish plastic particles by polymer type in complex air samples relevant to understanding human inhalation exposure to airborne MPs.