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Photothermal Heterodyne Imaging of Micron Sized Objects
Summary
Researchers tested a sensitive imaging technique called photothermal heterodyne imaging to detect and visualize micron-sized polymer beads similar in size to microplastic particles. The method shows promise as a non-destructive way to detect and characterize small plastic particles in various environments.
Micron sized dye-doped polymer beads were imaged using transmitted/reflected light microscopy and photothermal heterodyne imaging (PHI) measurements. The transmitted/reflected light images show distinct ring patterns that are attributed to diffraction effects and/or internal reflections within the beads. In the PHI experiments pump laser induced heating changes the refractive index and size of the bead, which causes changes in the diffraction pattern and internal reflections. This creates an analogous ring pattern in the PHI images. When the beads are imaged in an organic medium heat transfer changes the refractive index of the environment, and gives rise to a ring pattern external to the beads in the PHI images. This causes the beads to appear larger than their physical dimensions in the PHI experiments. This external signal does not appear when the beads are imaged in air, because the refractive index changes in air are very small.
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