Papers

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Article Tier 2

Bioaccumulation of differently-sized polystyrene nanoplastics by human lung and intestine cells

Researchers examined how human lung and intestine cells take up polystyrene nanoplastics of different sizes, finding that smaller particles were internalized in greater numbers but at lower total mass compared to larger ones. When compared on a surface area basis, the uptake rates were similar across sizes, suggesting that surface interactions with cell membranes play a key role. The findings indicate that particle size is an important factor to consider when evaluating the health risks of nanoplastic exposure.

2022 Journal of Hazardous Materials 86 citations
Article Tier 2

Internalization and toxicity: A preliminary study of effects of nanoplastic particles on human lung epithelial cell

Researchers studied the effects of polystyrene nanoplastic particles on human lung cells and found that the particles were internalized by the cells and caused dose-dependent toxicity. The nanoplastics triggered oxidative stress, inflammation, and disrupted normal cell function. The findings suggest that inhaling airborne nanoplastics may pose risks to respiratory health.

2019 The Science of The Total Environment 613 citations
Article Tier 2

Cellular internalization and release of polystyrene microplastics and nanoplastics

Scientists studied how polystyrene plastic particles of different sizes enter and exit living cells. They found that particles 50 and 500 nanometers in size can penetrate cell membranes and get taken up through multiple pathways, while 5-micrometer particles are too large to enter cells. This research helps explain why smaller nanoplastics may be more harmful to human health, as they can more easily get inside our cells and accumulate there.

2021 The Science of The Total Environment 373 citations
Article Tier 2

Uptake of Breathable Nano- and Micro-Sized Polystyrene Particles: Comparison of Virgin and Oxidised nPS/mPS in Human Alveolar Cells

Researchers compared uptake of virgin and oxidized polystyrene nano- and microparticles in human lung cells, finding that photoaged particles showed altered surface chemistry and different cellular internalization patterns relevant to realistic airborne microplastic exposure.

2023 Toxics 10 citations
Article Tier 2

Interactions between polystyrene nanoparticles and human intestinal epithelial Caco-2 cells

Researchers traced how 70 nm polystyrene nanoplastics enter and exit human intestinal Caco-2 cells, finding that particles accumulate in lysosomes and mitochondria over 72 hours and are cleared primarily through the lysosomal pathway, with serum in the medium inhibiting that clearance.

2025 NanoImpact 2 citations
Article Tier 2

Correlation between cellular uptake and cytotoxicity of polystyrene micro/nanoplastics in HeLa cells: A size-dependent matter

Researchers tested polystyrene particles of various sizes on human cells and found that only the smallest nanoplastics, those under about 25 nanometers in radius, could enter cells and cause toxic effects. Larger microplastic particles did not penetrate the cell membrane and showed no toxicity even at very high concentrations. The study provides a clear explanation for why smaller plastic particles tend to be more harmful, directly linking cell entry to cellular damage.

2023 PLoS ONE 29 citations
Article Tier 2

Recognition and movement of polystyrene nanoplastics in fish cells

Researchers tracked how zebrafish cells take up, transport, and release three types of polystyrene nanoplastics with different surface modifications. They found that cell uptake peaked within two hours and occurred mainly through specific cellular pathways, with the particles initially entering the cytoplasm before being transported to lysosomes. The nanoplastics were retained in cells for 10 to 15 hours depending on surface chemistry, highlighting the importance of understanding how these particles move through biological systems.

2022 Environmental Pollution 40 citations
Article Tier 2

Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells

Researchers tested how polystyrene nanoparticles interact with human intestinal cells in the lab. They found that the nanoparticles were readily taken up by the cells in a concentration-dependent manner, but no significant toxic effects were observed under the conditions tested. The study suggests that while nanoplastics can enter gut cells, their short-term toxicity at the tested levels appears limited.

2019 Environmental Science Nano 203 citations
Article Tier 2

Hazard Assessment of Polystyrene Nanoplastics in Primary Human Nasal Epithelial Cells, Focusing on the Autophagic Effects

Researchers exposed primary human nasal epithelial cells to polystyrene nanoplastics of two sizes and found that the smaller particles caused more significant cellular changes, including activation of autophagy pathways. The nanoplastics triggered oxidative stress and altered cell processes related to waste recycling within cells. The study highlights the potential health risks of inhaling airborne nanoplastics, an exposure route that remains understudied.

2023 Biomolecules 44 citations
Article Tier 2

Quantification of Polystyrene Uptake by Different Cell Lines Using Fluorescence Microscopy and Label-Free Visualization of Intracellular Polystyrene Particles by Raman Microspectroscopic Imaging

Scientists tested how human cells take up polystyrene microplastic particles using three cell types that represent the lung lining, intestinal lining, and immune system. All three cell types absorbed the microplastic beads, with immune cells showing different uptake patterns compared to the barrier cells of the lungs and gut. This study confirms that microplastics can enter human cells through multiple exposure routes, including breathing and eating, and that immune cells may play a special role in processing these particles.

2024 Cells 12 citations
Article Tier 2

Human neurons are susceptible to the internalization of small-sized nanoplastics

Researchers studied how human neurons take up nanoplastics and found that the cells readily absorbed 50-nanometer polystyrene particles through specific cellular pathways. The nanoplastics accumulated in cell compartments and, at higher concentrations, triggered oxidative stress and reduced cell survival. The study provides evidence that very small plastic particles can enter human brain cells, raising concerns about potential neurological effects of nanoplastic exposure.

2025 Environmental Toxicology and Pharmacology 4 citations
Article Tier 2

Uptake and Toxicity of Polystyrene NPs in Three Human Cell Lines

Researchers tested three sizes of polystyrene nanoparticles on human gut and liver cell lines and found that smaller particles (30 nm) were more toxic than larger ones (100 nm). Despite significant uptake by all cell types, the overall toxicity was low at tested concentrations. Interestingly, the nanoparticles entered cells mainly through a scavenger receptor pathway rather than the commonly assumed routes, which could change how scientists predict nanoplastic behavior in the human body.

2025 International Journal of Molecular Sciences 8 citations
Article Tier 2

Tissue distribution of polystyrene nanoplastics in mice and their entry, transport, and cytotoxicity to GES-1 cells

Scientists tracked polystyrene nanoplastics in mice after oral exposure and found the particles accumulated in the stomach, intestines, and liver tissues. In human gastric cells, the nanoplastics entered through multiple pathways and were transported through the cell's internal trafficking system, ultimately reducing cell growth and increasing cell death. The study provides detailed evidence of how nanoplastics can cross biological barriers and cause cellular damage in mammalian systems.

2021 Environmental Pollution 154 citations
Article Tier 2

[Exposure Pathways of Polystyrene Nanoplastics Mediate Their Cellular Distribution and Toxicity].

This study found that the route by which polystyrene nanoplastics enter the body determines which liver cell types accumulate the particles and what toxic effects occur, demonstrating that exposure pathway—not just dose—shapes nanoplastic toxicity in hepatic tissue.

2025 PubMed
Article Tier 2

Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells

Researchers studied how human intestinal Caco-2 cells take up polystyrene plastic particles of five different sizes ranging from 300 nanometers to 6 micrometers. The study found that smaller particles were taken up at higher rates and that co-exposure with bisphenol A increased cellular toxicity, suggesting that nanoscale plastics may pose a greater risk to human intestinal cells than larger microplastics.

2020 Chemosphere 267 citations
Article Tier 2

Cell uptake of mixtures of different-sized nanoplastics: Interplay and mechanism

Researchers studied how two sizes of polystyrene nanoplastics interact during cellular uptake, finding that larger 100 nm particles can pull smaller 50 nm particles into cells via clathrin-mediated endocytosis, while smaller particles alter the protein corona of larger ones in serum, either enhancing or inhibiting uptake depending on concentration ratios.

2026 Journal of Hazardous Materials
Article Tier 2

Uptake and toxicity of polystyrene micro/nanoplastics in gastric cells: Effects of particle size and surface functionalization

Researchers evaluated the uptake and toxicity of polystyrene micro- and nanoplastics in human gastric cells, comparing different sizes and surface treatments. The study found that smaller 50-nanometer particles were taken up at significantly higher rates, with positively charged aminated particles being the most toxic, causing cytotoxicity at lower concentrations and higher rates of cell death.

2021 PLoS ONE 96 citations
Article Tier 2

Pulmonary hazards of nanoplastic particles: a study using polystyrene in in vitro models of the alveolar and bronchial epithelium

Lab tests on human lung cell models found that polystyrene nanoplastics did not cause immediate cell death but did interfere with key lung functions like surfactant and mucus production and immune signaling. This means standard toxicity tests may underestimate the real danger of inhaling nanoplastics, and researchers need to look beyond simple cell survival to understand the true health effects on the lungs.

2025 Journal of Nanobiotechnology 13 citations
Article Tier 2

Systematic toxicity evaluation of polystyrene nanoplastics on mice and molecular mechanism investigation about their internalization into Caco-2 cells

Researchers fed mice polystyrene nanoplastics (about 100 nm) for 28 days and found the particles accumulated in multiple organs including the spleen, lungs, kidneys, intestines, testes, and brain. The nanoplastics caused cell death, inflammation, and tissue damage in these organs, as well as disrupted fat metabolism and blood cell counts. This study demonstrates that ingested nanoplastics can spread throughout the body and cause widespread harm, raising concerns about long-term human exposure.

2021 Journal of Hazardous Materials 335 citations
Article Tier 2

Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo

Researchers studied the uptake and effects of orally ingested polystyrene microplastic particles using human intestinal cell models and rodent experiments. They found that smaller microplastics were taken up by intestinal cells and could cross the gut barrier, though the majority passed through the digestive system. The study suggests that while most ingested microplastics are excreted, a fraction can be absorbed, warranting further investigation into long-term health effects.

2019 Archives of Toxicology 559 citations
Article Tier 2

Size-Dependent PulmonaryToxicity and Whole-Body Distributionof Inhaled Micro/Nanoplastic Particles in Male Mice from Chronic Exposure

Researchers used a whole-body inhalation exposure system to chronically expose male mice to polystyrene micro- and nanoplastics at environmental concentrations and tracked particle distribution and lung toxicity. Nanoplastics (80 nm) showed greater tissue transport than microplastics (1 µm), with highest accumulation in lungs followed by blood and spleen, and both sizes disrupted oxidative balance and antioxidant defenses.

2025 Figshare
Article Tier 2

Polystyrene nanoplastics increase migration in normal lung cells while inducing differential cytotoxicity in lung cancer cells

Researchers exposed normal and cancerous human lung cell lines to polystyrene nanoplastics (50–1000 nm) and found that while normal cells showed increased migration, cancer cells exhibited variable cytotoxicity, highlighting cell-type-specific responses to nanoplastic exposure.

2025 Toxicology
Article Tier 2

Polystyrene microparticle distribution after ingestion by murine macrophages

Researchers tracked what happens to polystyrene microparticles after they are ingested by mouse immune cells called macrophages. They found that the particles were distributed unevenly during cell division in a cell-type-specific manner, and no active excretion of the microplastics was observed. The study suggests that once immune cells take up microplastic particles, the particles may persist inside cells and accumulate over successive generations of cell division.

2023 Journal of Hazardous Materials 17 citations
Article Tier 2

Lysosomal dependent transcytosis of polystyrene nanoplastics within macrophages

Researchers studied how nanoplastics are transported through human macrophages via lysosomal-dependent transcytosis, tracking the complete intracellular migration pathway including internalization, vesicular trafficking, and exocytosis. The findings reveal that macrophages can transport nanoplastics across cellular barriers, potentially facilitating their distribution to distant tissues.

2025 Food and Chemical Toxicology