We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Papers
61,005 resultsShowing papers similar to Sample decomposition to determine microplastics in parts of mullets
ClearEvaluation and optimisation of sample preparation protocols suitable for the analysis of plastic particles present in seafood
Ten digestion protocols for extracting microplastics from seafood were compared across a broad range of species, evaluating their ability to fully digest tissue without damaging polymer integrity, providing a basis for selecting suitable methods for human exposure assessment.
Quick and efficient microplastic isolation from fatty fish tissues by surfactant-enhanced alkaline digestion
Researchers developed a faster method for extracting microplastics from fish tissues using a combination of detergents and potassium hydroxide, completing digestion of 100-gram samples within 16 to 24 hours — far quicker than existing multi-day protocols. The technique reliably recovered six common plastic types down to 60-80 micrometers in fatty fish like mackerel and salmon, making routine food safety monitoring more practical.
Improved methodology for microplastic extraction from gastrointestinal tracts of fat fish species
Researchers improved microplastic extraction from fatty fish tissues by adding a small amount of the surfactant Tween-20 to potassium hydroxide digestion protocols, which disrupts the soap layer that forms during fat digestion and significantly increases microplastic recovery rates.
Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project
An interlaboratory comparison tested two methods for extracting microplastics from fish gastrointestinal tracts and mussel tissue: 15% hydrogen peroxide digestion and 10% potassium hydroxide digestion. The exercise, conducted across four labs within the Plastic Busters MPAs project, aimed to harmonize extraction protocols for more comparable results.
Oil extraction following digestion to separate microplastics from mussels
This study compared several chemical digestion and extraction methods for isolating microplastics from mussel tissue, finding that hydrogen peroxide digestion followed by oil-based extraction achieved over 95% recovery across multiple plastic types. Optimized extraction methods are essential for accurately detecting microplastic contamination in shellfish that humans consume.
Improving the efficiency of post-digestion method in extracting microplastics from gastrointestinal tract and gills of fish
Researchers compared three post-digestion techniques for separating microplastics from fish tissue samples and found sieving and zinc chloride density separation both achieved over 95% efficiency without damaging most polymer types. The study provides practical guidance for improving the accuracy of microplastic detection in marine biological samples.
Food preference determines the best suitable digestion protocol for analysing microplastic ingestion by fish
Different fish species with different diets require different chemical digestion methods for accurately extracting and identifying microplastics from their digestive tracts. Choosing the right protocol for each species is essential for avoiding underestimation of microplastic ingestion, which affects the accuracy of food safety assessments.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
Assessing the effectiveness of microplastic extraction methods on fishmeal with different properties
Researchers evaluated microplastic extraction methods across five fishmeal types varying in protein, organic, carbonate, and density composition, finding that a calcium chloride overflow with dispersant and potassium hydroxide digestion achieved the highest recovery rate (66.3% in sardine and anchovy meal) and concluding that previously reported microplastic concentrations in fishmeal are likely underestimated due to inadequate methodology.
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
Researchers developed and tested a new protocol for the extraction and characterization of microplastics from environmental samples, optimizing steps for recovery efficiency and polymer identification accuracy.
Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.
Customized digestion protocols for copepods, euphausiids, chaetognaths and fish larvae facilitate the isolation of ingested microplastics
Researchers developed optimized, low-cost tissue digestion protocols using potassium hydroxide and enzymes to reliably extract ingested microplastics from tiny marine animals including copepods, krill, arrow worms, and fish larvae — organisms that are critical links in ocean food webs. The protocols achieved over 98% tissue digestion and over 91% microplastic recovery without damaging the plastic particles. Standardized methods for small zooplankton are urgently needed to understand how microplastics move up marine food chains and ultimately reach fish and seafood consumed by humans.
An assessment workflow to recover microplastics from complex biological matrices
Researchers developed an assessment workflow for recovering microplastics from complex marine biological matrices, finding that potassium hydroxide digestion was most effective across coral, sponge, sea squirt, and sea cucumber tissues while minimizing damage to plastic particles.
Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers developed and optimized an analytical extraction protocol for microplastics from seafood samples with varying fat content, testing the method on fresh and canned fish and molluscs. They found that fat-rich matrices required additional lipid removal steps to achieve reliable extraction efficiency, and validated a protocol suitable for high-fat seafood that reduces matrix interference while maintaining microplastic recovery.
Towards harmonised methods for microplastic analysis in food: development and optimisation for seafood products
Researchers evaluated and optimized methods for detecting and quantifying microplastics in the edible parts of seafood products, comparing enzymatic-alkaline digestion combined with filtration and oxidative treatment against other approaches for sensitivity, precision, and practical accessibility. The study aimed to contribute to method standardization, identifying the combined digestion protocol as most suitable for routine food control analysis.
Optimization of a new multi-reagent procedure for quantitative mussel digestion in microplastic analysis
This study optimized a chemical digestion protocol for extracting microplastics from mussel tissue by testing combinations of multiple reagents, aiming to completely destroy biological material while preserving plastic particles for analysis. The optimized method improved microplastic recovery compared to single-reagent approaches. Accurate extraction methods are critical for reliably measuring microplastic contamination in mussels, a widely consumed shellfish and important food safety indicator.
Protocol for extraction and analysis of microplastics in freshwater, sediment, and fish samples
Researchers developed a standardized protocol for extracting and identifying microplastics from freshwater, sediment, and fish digestive system samples, combining chemical digestion, density separation, vacuum filtration, and Raman spectroscopy to enable reliable, reproducible analysis across aquatic sample types.
Microplastics in wild mussels (Mytilus spp.) from the north coast of Spain
Mussels from two regions of northern Spain's coastline contained microplastics, with potassium hydroxide digestion recovering significantly more particles than nitric acid digestion. The choice of digestion method substantially affects the number of microplastics detected in seafood, making methodological standardization critical for food safety assessments.
Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers optimized an analytical protocol for extracting microplastics from seafood samples with varying fat content, addressing a key methodological challenge in accurately quantifying microplastic contamination in marine food sources given the ubiquitous presence of plastic particles smaller than 5 mm in marine environments.
Screening for microplastics in sediment, water, marine invertebrates and fish: Method development and microplastic accumulation
Researchers developed improved methods for extracting microplastics from biological samples and sediments, using enzymatic digestion that achieved 97% particle recovery without damaging the plastics. When applied to field samples from the North Sea and Swedish coast, they found microplastics in 8 of 9 invertebrate species tested and in 68% of brown trout. Mussel tissue contained roughly a thousand times more microplastic particles per kilogram than surrounding sediment or water.
Nondestructive Extraction and Identification of Microplastics from Freshwater Sport Fish Stomachs
Researchers developed a nondestructive protocol for extracting and identifying microplastics from freshwater sport fish stomachs containing large amounts of biological material, enabling more accurate counts without damaging the plastics. This method improvement helps ensure that ingested microplastics in fish are not missed or damaged during laboratory processing.
A new digestion approach for the extraction of microplastics from gastrointestinal tracts (GITs) of the common dolphinfish (Coryphaena hippurus) from the western Mediterranean Sea
Researchers developed a novel combined KOH and nitric acid digestion protocol for extracting microplastics from the gastrointestinal tracts of marine fish, and validated it on common dolphinfish from the western Mediterranean Sea. The method improved detection of small MPs, with 65.5% of dolphinfish containing meso- and microplastic fragments and sheets predominantly composed of polyethylene, polypropylene, and polystyrene.
A rapid method for extracting microplastics from oily food samples
Researchers developed a rapid method for extracting microplastics from oily food samples, addressing a key challenge for detecting plastic contamination in foods like fish that contain high fat content. Standardized extraction methods for oily food matrices are needed to accurately assess dietary microplastic exposure.
Methods of digestion, isolation and identification of microplastics present in the fish gut content
This study evaluated four digestion methods for isolating microplastics from fish gut contents, finding that a combination of nitric acid and hydrofluoric acid was most effective while preserving particle structure. Microplastic particles were found in the digestive tracts of all analyzed fish from Croatian freshwater and marine ecosystems, highlighting the widespread contamination of aquatic food sources.