Papers

61,005 results
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Article Tier 2

Toxicological effects of microplastics and heavy metals on the Daphnia magna

Researchers studied how polystyrene microplastics of two sizes adsorb heavy metals and how their combined presence affects the water flea Daphnia magna. They found that smaller microplastics had higher adsorption capacity for metals, and the combined toxicity shifted from antagonistic to additive effects as microplastic concentrations increased. The study reveals that smaller microplastics pose a greater toxicological risk when combined with heavy metals in aquatic environments.

2020 The Science of The Total Environment 184 citations
Article Tier 2

Evaluating additive versus interactive effects of copper and cadmium on Daphnia pulex life history

This study assessed how two common heavy metals — copper and cadmium — combine to affect the water flea Daphnia pulex under different food conditions and across multiple genotypes. Understanding metal mixture toxicity is relevant to assessing microplastic risk because plastics often carry multiple metals adsorbed from seawater.

2019 Environmental Science and Pollution Research 13 citations
Article Tier 2

Impacts of microplastics and pesticides on Daphnia

Researchers investigated the combined and individual impacts of microplastics and pesticides on Daphnia magna, a model crustacean widely used in freshwater ecotoxicology, to assess how these co-occurring pollutants affect aquatic ecosystem health. The study examined survival, reproduction, and physiological responses in D. magna exposed to varying concentrations of both stressors under controlled conditions.

2025 Fisher Digital Publications (St. John Fisher College)
Article Tier 2

Short- and long-term single and combined effects of microplastics and chromium on the freshwater water flea Daphnia magna

Researchers investigated the individual and combined effects of microplastics and chromium on the water flea Daphnia magna in both short- and long-term experiments. They found that microplastics interacted with chromium, reducing its concentration in water, and that co-exposure caused acute toxicity but lacked the chronic effects seen with chromium alone. The study suggests that microplastics may alter the bioavailability and toxicity of metal pollutants in freshwater environments.

2022 Aquatic Toxicology 44 citations
Article Tier 2

Mixture Toxicity of Nickel and Microplastics with Different Functional Groups on Daphnia magna

Researchers investigated the combined toxicity of nickel and two types of polystyrene microplastics with different surface chemistries on Daphnia magna. They found that the presence of microplastics altered the toxicity of nickel, with surface functional groups playing an important role in determining the severity of combined effects. The study demonstrates that microplastics can modify the bioavailability and toxicity of heavy metals in freshwater environments.

2017 Environmental Science & Technology 282 citations
Article Tier 2

Synergistic effect of microplastic fragments and benzophenone‐3 additives on lethal and sublethal Daphnia magna toxicity

Researchers assessed the combined effects of polyethylene microplastic fragments and the UV-filter additive benzophenone-3 on the water flea Daphnia magna. They found that microplastic fragments were significantly more acutely toxic than the dissolved additive alone, and the combination produced synergistic lethal and sublethal effects. The study highlights that microplastic particles carrying chemical additives may pose greater risks to aquatic invertebrates than either stressor in isolation.

2020 Journal of Hazardous Materials 120 citations
Article Tier 2

Effects of Exposure to Cadmium, Microplastics, and Their Mixture on Survival, Growth, Feeding, and Life History of Daphnia magna

Researchers examined how polyethylene microplastics altered cadmium toxicity to Daphnia magna, finding that microplastic co-exposure modified cadmium bioavailability and affected survival, growth, feeding rates, and reproductive outcomes in this ecologically important species.

2023 Environmental Toxicology and Chemistry 9 citations
Article Tier 2

The Effect of Gadolinium and Lanthanum on the Mortality of Daphnia magna

This study assessed the toxicity of two rare earth elements—gadolinium and lanthanum—to the water flea Daphnia magna, finding both were harmful at environmentally relevant concentrations. Rare earth elements are increasingly released into water from electronics and technology manufacturing, and their effects on aquatic organisms are poorly understood.

2021 International Journal of Innovative Approaches in Agricultural Research 2 citations
Article Tier 2

The synergistic effect of mono and co-exposure of microplastic suspensions on Daphnia magna’s survival, population density, reproduction rate & swimming behavior.

When water fleas (Daphnia magna) were exposed to mixtures of HDPE, LDPE, and polypropylene microplastics together, the combined toxicity was substantially greater than any single polymer alone, with the mixture LC50 dropping to 77 mg/L compared to 109–123 mg/L for individual plastics. This synergistic effect — reducing survival, reproduction, and normal swimming behavior — is an important finding because organisms in nature encounter mixtures of plastic types, not just one at a time.

2023 Research Square (Research Square)
Article Tier 2

Acute toxicity of triclosan, caffeine, nanoplastics, microplastics, and their mixtures on Daphnia magna

Researchers tested the acute toxicity of triclosan, caffeine, nanoplastics, and microplastics individually and in mixtures on the water flea Daphnia magna. They found that nanoplastics were more toxic than microplastics, and mixtures of these pollutants with triclosan or caffeine produced varying levels of combined toxicity. The study highlights that environmental pollutants rarely occur in isolation, and their mixtures may have unpredictable effects on aquatic organisms.

2023 Marine Pollution Bulletin 29 citations
Article Tier 2

Action of Surfactants in Driving Ecotoxicity of Microplastic-Nano Metal Oxides Mixtures: A Case Study on Daphnia magna under Different Nutritional Conditions

This study tested how surfactants (found in detergents and cleaning products) interact with microplastics and metal oxide nanoparticles to affect the toxicity of these combined pollutants on water fleas (Daphnia magna). Surfactants increased the toxicity of microplastic-nanoparticle mixtures, and the effect varied with the age and nutritional status of the test organisms. This highlights how the complex real-world mixture of pollutants in waterways can be more harmful than any single contaminant alone.

2021 IntechOpen eBooks 2 citations
Article Tier 2

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Researchers used daphnids to assess the impact of eight chemicals individually and as a mixture, finding that composite mixtures significantly enhanced toxicity and that enzyme markers combined with metabolomics can sensitively detect pollution effects.

2022 Toxics 24 citations
Article Tier 2

Assessment of intake and effect of microplastics and its combination with metals in experimental (Daphnia magna) and environmental conditions (freshwater fish)

Researchers assessed the intake and effects of microplastics and their combination with metals using Daphnia magna as an experimental model and freshwater fish under environmental conditions, investigating how microplastics adsorb and transport harmful metals in freshwater systems.

2025 Repository of Faculty of Chemical Engineering and Technology University of Zagreb
Article Tier 2

Cytotoxicity and Genotoxicity Effects of a Magnetic Zeolite Composite in Daphnia magna (Straus, 1820)

Researchers tested the toxicity of a magnetic zeolite composite material, designed for removing heavy metals from water, on the freshwater organism Daphnia magna. They determined lethal concentration thresholds and found that the composite caused both cytotoxic and genotoxic effects at certain concentrations. The study highlights the importance of evaluating the ecological safety of remediation materials before deploying them in natural water bodies.

2024 International Journal of Molecular Sciences 2 citations
Article Tier 2

The combined toxicity test of polyester and tetra ethylene glycol on Daphnia magna

This study tested the combined toxicity of polyester microplastics and tetraethylene glycol on the water flea Daphnia magna, a standard freshwater toxicity test organism. The combined exposure was more harmful than either substance alone, highlighting the risks of plastic-chemical mixtures in aquatic environments.

2021
Article Tier 2

Effects of natural organic matter on the joint toxicity and accumulation of Cu nanoparticles and ZnO nanoparticles in Daphnia magna

Researchers tested how copper and zinc oxide nanoparticle mixtures affect the water flea Daphnia magna in the presence and absence of natural organic matter, finding additive-to-synergistic joint toxicity and showing that natural organic matter shifts the dominant toxic species toward dissolved zinc ions while increasing nanoparticle accumulation in the organism's body.

2021 Environmental Pollution 27 citations
Article Tier 2

Effects of Single and Combined Ciprofloxacin and Lead Treatments on Zebrafish Behavior, Oxidative Stress, and Elements Content

Researchers assessed the combined acute effects of the antibiotic ciprofloxacin and lead on zebrafish at environmentally relevant concentrations. The study found that the mixture caused more severe impacts than individual substances, including reduced swimming activity, disrupted mineral balance, inhibited acetylcholinesterase activity, and increased oxidative stress. These findings highlight that the co-occurrence of antibiotics and heavy metals in aquatic environments may pose greater risks to organisms than either pollutant alone.

2023 International Journal of Molecular Sciences 25 citations
Article Tier 2

Combined effect of polystyrene nanoparticles and chlorpyrifos to Daphnia magna

This study examined the combined effects of polystyrene nanoparticles and chlorpyrifos pesticide on Daphnia magna, a standard aquatic toxicity test organism. The two contaminants together caused greater mortality and reproductive impairment than either alone, suggesting synergistic toxicity.

2024 Chemosphere 1 citations
Article Tier 2

Exposure scenarios modulate the combined toxicity of nanoplastics and Cd to Daphnia magna

Researchers evaluated how different exposure scenarios (single, pre-exposure, and co-exposure) affect the combined toxicity of nanoplastics and cadmium to the water flea Daphnia magna. The study found that environmentally relevant concentrations of nanoplastics initially stimulated reproduction, but the timing and sequence of exposure significantly influenced bioaccumulation, intestinal damage, and gut microbiota disruption.

2026 Environmental Pollution
Article Tier 2

Combined Effect of Polystyrene Particles and Copper Ions on the Vital Parameters of Daphnia Magna in a Series of Generations

Researchers studied the combined effects of polystyrene microplastics and copper ions on Daphnia magna over four generations using both short-term and continuous exposure designs. Toxicity was greater under combined exposure and increased across generations, suggesting cumulative intergenerational harm from mixed pollutant stress.

2025 Transactions of Papanin Institute for Biology of Inland Waters RAS
Article Tier 2

Effects of combined nutrient and pesticide exposure on algal biomass and Daphnia magna abundance

Researchers investigated the individual and combined effects of nutrients and pesticides on Daphnia magna abundance and algal biomass under controlled conditions, examining how agricultural inputs — fertilizers driving eutrophication and pesticides causing direct toxicity — interact to affect freshwater biodiversity.

2022 Research Square (Research Square) 2 citations
Article Tier 2

Combined effect of microplastics and tire particles on Daphnia magna: Insights from physiological and transcriptomic responses

Researchers investigated the combined effects of microplastics and tire particles on the water flea Daphnia magna, finding that the mixture triggered significant oxidative stress at environmentally relevant concentrations. Transcriptomic analysis revealed upregulation of antioxidant and metabolic stress genes, while energy reserves like glycogen were affected. The study suggests that co-exposure to these common freshwater pollutants may pose greater ecological risks than either particle type alone.

2025 Environmental Pollution 1 citations
Article Tier 2

Long-term effects of lithium and lithium-microplastic mixtures on the model species Daphnia magna: Toxicological interactions and implications to ‘One Health’

Researchers investigated long-term effects of lithium and lithium-microplastic mixtures on Daphnia magna, finding that combined exposure produced toxicological interactions different from individual exposures, with implications for freshwater ecosystem health under a One Health framework.

2022 The Science of The Total Environment 33 citations
Article Tier 2

Combined toxicity of perfluoroalkyl substances and microplastics on the sentinel species Daphnia magna: Implications for freshwater ecosystems

This study tested how PFAS chemicals (common industrial pollutants) and PET microplastics affect water fleas, both alone and together. The combination caused worse developmental and reproductive problems than either pollutant alone, and organisms with prior chemical exposure history responded differently, showing that microplastics can amplify the harm of other environmental contaminants in ways that are difficult to predict.

2024 Environmental Pollution 37 citations