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61,005 resultsShowing papers similar to Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals
ClearHyperspectral Oblique Plane Microscopy Enables Spontaneous, Label-Free Imaging of Biological Dynamic Processes in Live Animals
Researchers developed a single-objective light-sheet microscope called lambda-OPM that records spontaneous Raman spectral images at millisecond-to-minute timescales, demonstrating its ability to identify microplastic particles by polymer type and to capture real-time molecular changes in live zebrafish embryo wound healing and beating heart tissue.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
Researchers used spectral focusing hyperspectral stimulated Raman scattering (SRS) microscopy to longitudinally monitor microplastic uptake, size-dependent organ accumulation, and lipid metabolic changes in live zebrafish during development. They found that microplastic exposure disrupted hepatic lipid metabolism and energy homeostasis, with the SRS imaging approach enabling real-time, label-free tracking of microplastics and associated biochemical changes in living organisms.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Fast detection and 3D imaging of nanoplastics and microplastics by stimulated Raman scattering microscopy
Researchers developed a fast imaging technique using stimulated Raman scattering microscopy to detect and create 3D maps of nanoplastics and microplastics at the single-particle level. The method can identify plastic particles as small as 100 nanometers and distinguish between different polymer types without the need for dyes or labels. This technology could help scientists more accurately track tiny plastic particles in environmental and biological samples.
Visible Combined Near-Infrared in Situ Imaging Revealed Dynamic Effects of Microplastic Fibers and Beads in Zebrafish
Researchers used a combined visible and near-infrared imaging technique to track microplastic fibers and beads in live zebrafish in real time. They observed that microplastics were quickly ingested and could be retained briefly in the digestive tract before being eliminated. The study provides new insights into the dynamic behavior of microplastics inside living organisms and whether any tissue damage that occurs during transit can be reversed.
Label-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy
Label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy was used to simultaneously visualize internalized microplastics and cellular organelles in live cells without requiring fluorescent staining. The approach enables real-time tracking of plastic particle interactions with intracellular structures, offering new insight into how microplastics behave inside human cells.
Imaging and quantifying the biological uptake and distribution of nanoplastics using a dual-functional model material
Researchers developed a dual-functional nanoplastic model material that allows both imaging and precise quantification of nanoplastic uptake in biological systems. Using surface-enhanced Raman spectroscopy and inductively coupled plasma mass spectrometry, they could track where nanoplastics accumulated in organisms at high resolution. The tool addresses a major gap in nanoplastic research by enabling more accurate measurement of how these tiny particles interact with living tissues.
An aberration-free line scan confocal Raman imager and type classification and distribution detection of microplastics
Researchers developed an advanced Raman imaging system that can identify and classify microplastics as small as 1 micrometer in diameter with 98% accuracy, working about 100 times faster than traditional methods. The system can also detect harmful chemical residues like phthalate plasticizers on microplastic surfaces. Faster and more accurate detection tools like this are essential for understanding the full scope of microplastic contamination in food and water and its potential impact on human health.
Hyperspectral Imaging Based Method for Rapid Detection of Microplastics in the Intestinal Tracts of Fish
Researchers developed a hyperspectral imaging-based method to directly detect and identify microplastics in fish intestinal tracts without requiring tissue digestion or particle extraction, enabling faster and less reagent-intensive analysis compared to conventional Raman or FTIR approaches.
Confocal surface-enhanced Raman imaging of the intestine barrier crossing behavior of nanoplastics in Daphnia magna
Using a specially engineered nanoplastic particle visible under confocal Raman imaging, researchers tracked how nanoplastics move from the gut into other organs of the water flea Daphnia magna. The study revealed that nanoplastics can cross the intestinal barrier and translocate to other body parts, providing direct visual evidence of how these particles spread through a living organism and raising concerns about similar processes in other aquatic animals.
Direct monitoring of the enzymatically sequestering and degrading of PET microplastics using hyperspectral Raman microscopy
Scientists attached a plastic-degrading enzyme to magnetic nanoparticles, creating tiny agents that can capture and break down PET microplastics in water. Using a novel real-time imaging technique, they were able to directly observe the degradation process, demonstrating a promising nanotechnology approach for removing microplastic pollution from water.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Label-Free Identification and Imaging of Microplastic and Nanoplastic Biouptake Using Optical Photothermal Infrared Microspectroscopy
Researchers developed a new imaging technique that can locate and identify microplastic and nanoplastic particles inside whole organisms without needing fluorescent labels. Using a method called optical photothermal infrared microscopy, they tracked polystyrene particles as small as 1 micrometer in roundworms. This tool could help scientists better understand how plastic particles are taken up by living things and where they accumulate in the body.
Dark-field hyperspectral microscopy for label-free microplastics and nanoplastics detection and identification in vivo: A Caenorhabditis elegans study
Researchers demonstrated that dark-field hyperspectral microscopy can visualize and chemically identify nano- and microplastics (down to 100 nm) in live C. elegans nematodes without labeling, differentiating multiple polymer types simultaneously within intestinal tissue.
Near-Infrared-II In Vivo Visualization and Quantitative Tracking of Micro/Nanoplastics in Fish
Scientists developed a new near-infrared imaging technique to track micro- and nanoplastics inside living zebrafish in real time, overcoming limitations of previous detection methods. They found that both sizes of plastic particles accumulated mainly in the gut, with microplastics concentrating more in the front sections and nanoplastics distributing more evenly. This tracking tool helps researchers better understand how plastic particles move through and accumulate in living organisms, which is essential for assessing the risks of microplastic exposure.
Accumulation of nanoplastics in human cells as visualized and quantified by hyperspectral imaging with enhanced dark-field microscopy
Researchers developed a label-free imaging technique to visualize and count nanoplastic particles that accumulate inside human cells, using enhanced dark-field microscopy combined with hyperspectral imaging. The method successfully tracked polystyrene nanoplastics entering cells over time and measured accumulation rates without needing fluorescent labels. This tool could improve the accuracy of future studies assessing how nanoplastics build up in human tissue and what concentration levels may pose health risks.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Tissue Clearing To Localize Microplastics via Three-Dimensional Imaging of Whole Organisms
Researchers developed a tissue-clearing technique that renders whole organisms transparent after microplastic ingestion, allowing 3D fluorescence imaging to precisely locate unlabeled environmental microplastics inside an organism without destroying tissue. Unlike conventional digestion methods that lose spatial information, this approach preserves the organism's structure while a fluorescent dye selectively stains the plastics. This tool could substantially improve our understanding of where microplastics accumulate within living organisms and what tissues they affect.
Raman spectroscopy as the quantum eye to reveal molecular dynamics in biology
Researchers reviewed advances in Raman spectroscopy — a technique that identifies chemicals by how they scatter laser light — highlighting how recent innovations in surface-enhanced and stimulated Raman methods have expanded its applications in cell imaging, disease diagnosis, drug development, and microplastic detection.
Classification of microplastics in living organism using color polarization camera
Researchers developed a color polarization camera system to classify microplastics in living organisms, using polarization contrast imaging to distinguish plastic particles from biological tissue in vivo, enabling non-destructive detection of ingested MPs without sacrificing or chemically treating animals.
Fast microplastics identification with stimulated Raman scattering microscopy
Stimulated Raman scattering microscopy was applied to rapidly identify and image microplastic particles in complex environmental samples at speeds dramatically faster than conventional Raman spectroscopy. The technique has potential to enable high-throughput microplastic analysis that could make large-scale environmental monitoring more feasible.
Laser speckle imaging in discrimination of zooplanktons from supermicroplastics
Researchers developed a laser speckle imaging technique to distinguish tiny microplastic particles (under 350 µm) from zooplankton in water samples in real time. This new method could significantly improve the accuracy and speed of monitoring small microplastics in ocean environments.
Detection of unlabeled nanoplastics within Daphnia magna using enhanced darkfield hyperspectral microscopy
Researchers developed a method to detect unlabeled nanoplastics within Daphnia magna using enhanced darkfield hyperspectral microscopy combined with a post-exposure histological labeling process. The study presents a new approach for identifying nanoplastic particles in model organisms without requiring pre-labeled or fluorescent plastics.