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Hyperspectral Oblique Plane Microscopy Enables Spontaneous, Label-Free Imaging of Biological Dynamic Processes in Live Animals
Summary
Researchers developed a single-objective light-sheet microscope called lambda-OPM that records spontaneous Raman spectral images at millisecond-to-minute timescales, demonstrating its ability to identify microplastic particles by polymer type and to capture real-time molecular changes in live zebrafish embryo wound healing and beating heart tissue.
Abstract Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo , current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective light-sheet microscope, named λ -OPM, which records Raman images on a timescale of minutes to milliseconds. To demonstrate its function, we use λ -OPM to map and identify micro-plastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that λ -OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and extracellular matrix composition in the wounded region. Finally, we use λ -OPM to obtain Raman scattering maps of a zebrafish embryo’s beating heart at an effective 28 frames per second, recording compositional changes at different points in the cardiac cycle.
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