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Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals
Summary
Researchers developed a new high-speed Raman imaging microscope that can capture label-free, molecular-level images of biological processes in living animals. They demonstrated the technology by tracking microplastic accumulation in the organs of live zebrafish, visualizing particle distribution without any dyes or markers. This imaging advancement could significantly improve how scientists study microplastic uptake and distribution in living organisms.
Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named [Formula: see text]-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its function, we use [Formula: see text]-OPM to map and identify microplastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that [Formula: see text]-OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and extracellular matrix composition in the wounded region. Finally, we use [Formula: see text]-OPM to synchronize and average 36,800 individual frames to obtain hyperspectral videos of a zebrafish embryo's beating heart at an effective 28 frames per second, recording compositional changes throughout the cardiac cycle.