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Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals
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Researchers developed a new high-speed Raman imaging microscope that can capture label-free, molecular-level images of biological processes in living animals. They demonstrated the technology by tracking microplastic accumulation in the organs of live zebrafish, visualizing particle distribution without any dyes or markers. This imaging advancement could significantly improve how scientists study microplastic uptake and distribution in living organisms.
Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named [Formula: see text]-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its function, we use [Formula: see text]-OPM to map and identify microplastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that [Formula: see text]-OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and extracellular matrix composition in the wounded region. Finally, we use [Formula: see text]-OPM to synchronize and average 36,800 individual frames to obtain hyperspectral videos of a zebrafish embryo's beating heart at an effective 28 frames per second, recording compositional changes throughout the cardiac cycle.
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Hyperspectral Oblique Plane Microscopy Enables Spontaneous, Label-Free Imaging of Biological Dynamic Processes in Live Animals
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Researchers developed a single-objective light-sheet microscope called lambda-OPM that records spontaneous Raman spectral images at millisecond-to-minute timescales, demonstrating its ability to identify microplastic particles by polymer type and to capture real-time molecular changes in live zebrafish embryo wound healing and beating heart tissue.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
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Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Label-free 4D confocal Raman imaging enabling in vivo multiplex identification of microplastics in zebrafish
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Scientists created a new imaging technique that can track five different types of microplastics inside living zebrafish at the same time, without needing to add dyes or chemical tags that could alter the results. They found that different plastic types build up in the gut at different rates, with noticeable accumulation after just 8 hours of exposure, a tool like this could help researchers better understand how the mix of plastics we're exposed to in real life actually moves through and affects living bodies, which matters since humans regularly ingest microplastics from food and water.
Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
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Researchers used spectral focusing hyperspectral stimulated Raman scattering (SRS) microscopy to longitudinally monitor microplastic uptake, size-dependent organ accumulation, and lipid metabolic changes in live zebrafish during development. They found that microplastic exposure disrupted hepatic lipid metabolism and energy homeostasis, with the SRS imaging approach enabling real-time, label-free tracking of microplastics and associated biochemical changes in living organisms.
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