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20 resultsShowing papers similar to Single‐Cell Transcriptomic Analysis Reveals Hair Cell‐Specific Molecular Responses to Polystyrene Nanoplastics in a Zebrafish Embryo Model
ClearSingle-Cell RNA Sequencing Reveals Size-Dependent Effects of Polystyrene Microplastics on Immune and Secretory Cell Populations from Zebrafish Intestines
Researchers used single-cell RNA sequencing to examine how different sizes of polystyrene microplastics affect intestinal cell populations in zebrafish. The study revealed size-dependent effects on immune and secretory cell populations, providing a detailed transcriptomic view of how microplastics disrupt intestinal function at the individual cell level and alter the interplay between intestinal cells and gut microbiota.
Single-cell transcriptomic analysis reveals heterogeneity of the patterns of responsive genes and cell communications in liver cell populations of zebrafish exposed to polystyrene nanoplastics
Researchers used single-cell gene analysis to examine how polystyrene nanoplastics affect different cell types in zebrafish livers. They discovered that various liver cell populations responded to nanoplastic exposure in distinctly different ways, with some cell types showing more disruption to fat metabolism and stress response genes than others. The study reveals that nanoplastic toxicity in the liver is not uniform and that certain cell populations may be more vulnerable than previously understood.
Effects of Nanoplastics and Butyl Methoxydibenzoylmethane on Early Zebrafish Embryos Identified by Single-Cell RNA Sequencing
Researchers used single-cell RNA sequencing to study how polystyrene nanoplastics and the sunscreen chemical BMDBM affect early zebrafish embryo development. The study found that both pollutants targeted neural cells and disrupted brain development pathways, though combined exposure appeared to reduce some adverse effects compared to individual exposures, highlighting the complexity of nanoplastic interactions with co-occurring contaminants.
Nanoplastics impact the zebrafish (Danio rerio) transcriptome: Associated developmental and neurobehavioral consequences
Researchers exposed developing zebrafish larvae to polystyrene nanoplastics of two sizes and found dose-dependent accumulation in tissues along with swimming hyperactivity, despite no effects on mortality or hatching. Transcriptomic analysis revealed changes in gene expression associated with neurodegeneration and motor dysfunction at both high and low concentrations. The study suggests that nanoplastic exposure during early development can alter brain function and behavior in ways that may reduce organismal fitness.
Ototoxicity of polystyrene nanoplastics in mice, HEI-OC1 cells and zebrafish
Researchers discovered that polystyrene nanoplastics can enter auditory organs and cause hearing-related damage in mice, cell cultures, and zebrafish. The nanoplastics triggered cell death and oxidative stress in hair cells responsible for detecting sound, leading to measurable hearing impairment. The study identifies the auditory system as a previously unrecognized target of nanoplastic toxicity, suggesting that exposure to these particles may pose risks to hearing health.
Transcriptome sequencing and metabolite analysis reveal the toxic effects of nanoplastics on tilapia after exposure to polystyrene
Researchers exposed larval tilapia to polystyrene nanoplastics and then analyzed changes in gene expression and metabolic profiles after a recovery period. They found that nanoplastic exposure disrupted immune-related pathways, energy metabolism, and lipid processing in the fish, with some effects persisting even after exposure ended. The study suggests that nanoplastics can cause lasting metabolic and immune disruptions in freshwater fish.
Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
Researchers exposed zebrafish embryos to polystyrene nanoplastics at various concentrations and measured gene expression changes related to stress, inflammation, and DNA repair. They found dose-dependent activation of oxidative stress and apoptotic pathways at the highest concentration, along with inhibition of the neurotransmitter-related gene acetylcholinesterase and DNA repair genes. The study suggests that nanoplastic exposure at the molecular level may compromise cellular defense mechanisms and neurological function in developing fish.
Polystyrene nanoplastic induces oxidative stress, immune defense, and glycometabolism change in Daphnia pulex: Application of transcriptome profiling in risk assessment of nanoplastics
Researchers used transcriptome sequencing to examine how polystyrene nanoplastics affect gene expression in the water flea Daphnia pulex. After 96 hours of exposure, they identified 208 genes with altered expression levels, linked to oxidative stress, immune defense, and sugar metabolism pathways. The study provides molecular-level evidence that nanoplastic pollution can trigger multiple stress responses in freshwater organisms.
Polystyrene nanoplastics affect transcriptomic and epigenomic signatures of human fibroblasts and derived induced pluripotent stem cells: Implications for human health
Researchers found that polystyrene nanoplastics altered transcriptomic and epigenomic signatures in human fibroblasts and derived induced pluripotent stem cells, demonstrating that plastic particle exposure can cause lasting molecular changes with potential implications for human health.
Sequencing data of Amphiprion ocellaris (clownfish) exposed to polystyrene nanoplastic
Researchers exposed clownfish to polystyrene nanoplastics at environmentally relevant and high concentrations for seven days and performed biochemical and transcriptomic analyses. The study found that even low-concentration exposure triggered significant gene expression changes indicating energy reallocation and stress responses, while high concentrations amplified these effects and activated additional inflammatory and detoxification pathways.
Sequencing data of Amphiprion ocellaris (clownfish) exposed to polystyrene nanoplastic
Researchers exposed clownfish to polystyrene nanoplastics at environmentally relevant and high concentrations for seven days and performed biochemical and transcriptomic analyses. The study found that even low-concentration exposure triggered significant gene expression changes indicating energy reallocation and stress responses, while high concentrations amplified these effects and activated additional inflammatory and detoxification pathways.
Single-Cell RNA Sequencing Profiling Cellular Heterogeneity and Specific Responses of Fish Gills to Microplastics and Nanoplastics
Using advanced single-cell sequencing, researchers mapped how individual cell types in fish gills respond differently to micro- and nanoplastic exposure. Microplastics mainly affected immune cells called macrophages, while nanoplastics primarily targeted T cells, and a structural cell type called fibroblasts was especially sensitive to microplastics. This detailed cell-level view reveals that plastic particles of different sizes can trigger distinct immune and tissue responses.
Transcriptional effects of polyethylene microplastics ingestion in developing zebrafish (Danio rerio)
Researchers exposed developing zebrafish to polyethylene microplastics and used transcriptomic analysis to identify changes in gene expression related to immune function, lipid metabolism, and oxidative stress. The study suggests that even at relatively low concentrations, ingested microplastics can alter key biological pathways during early fish development.
4 Single cell RNA-seq samples exposed to nano plastic particles
Researchers used microfluidic chip-based single-cell RNA sequencing to profile the transcriptional responses of human peripheral blood immune cells exposed to carboxylated polystyrene nanoparticles of three sizes (40 nm, 200 nm, or a mixture), providing a cell-type-resolved view of nanoplastic effects on the immune system.
Transcriptional response provides insights into the effect of chronic polystyrene nanoplastic exposure on Daphnia pulex
RNA sequencing of Daphnia pulex after 21 days of polystyrene nanoplastic exposure identified 244 differentially expressed genes, with key downregulated genes involved in trehalose metabolism and chitin synthesis and upregulated genes involved in stress response pathways. The transcriptomic analysis reveals metabolic and immune disruption as central mechanisms of chronic nanoplastic toxicity in this keystone freshwater species.
Polystyrene nanoplastics disrupt ovarian development via cytoskeletal remodeling and epigenetic reprogramming particularly in granulosa cells
Researchers used single-cell RNA sequencing to map polystyrene nanoplastic toxicity in mouse ovaries, identifying granulosa cells as the primary target and showing that 100 nm particles trigger F-actin cytoskeletal remodeling, STAT1-driven epigenetic reprogramming, and necroptosis, disrupting follicle development and hormone production.
Pathway analysis of systemic transcriptome responses to injected polystyrene particles in zebrafish larvae
Researchers injected fluorescent polystyrene particles into zebrafish embryos at different developmental stages and tracked their distribution and biological effects using imaging and transcriptomics. Particles injected into the yolk of older embryos spread through the bloodstream and accumulated near the heart, triggering strong immune and inflammatory gene responses. The study reveals that even localized microplastic exposure can produce system-wide biological effects in developing organisms.
Effects of Acute Exposure to Polystyrene Nanoplastics on the Channel Catfish Larvae: Insights From Energy Metabolism and Transcriptomic Analysis
Researchers found that acute exposure to polystyrene nanoplastics disrupted energy metabolism in channel catfish larvae, with transcriptomic analysis revealing altered gene expression in pathways related to oxidative stress and metabolic processes.
Heterogeneity effects of nanoplastics and lead on zebrafish intestinal cells identified by single-cell sequencing
Researchers used single-cell RNA sequencing to examine how polystyrene nanoplastics and lead individually and together affect different cell types in zebrafish intestines. They found that nanoplastics primarily affected macrophages while lead mostly impacted enterocytes, and the combined exposure showed synergistic effects specifically in goblet cells. The study reveals that population-average toxicity measurements can mask important cell-type-specific responses to environmental contaminants.
Toxicological impacts of nanopolystyrene on zebrafish oocyte with insight into the mechanism of action: An expression-based analysis
Researchers investigated the mechanism by which nanopolystyrene causes toxicity in zebrafish oocytes, finding it triggers oxidative stress, immune disruption, and mitochondrial dysfunction through changes in key gene expression pathways.