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Transcriptional response provides insights into the effect of chronic polystyrene nanoplastic exposure on Daphnia pulex

Chemosphere 2019 125 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 50 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Zhiquan Liu, Qichen Jiang Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Qichen Jiang Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Shengkai Tang, Zhiquan Liu, Zhiquan Liu, Wenyi Zhang, Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Zhiquan Liu, Wenyi Zhang, Zhiquan Liu, Zhiquan Liu, Qichen Jiang Qichen Jiang Zhiquan Liu, Qichen Jiang Zhiquan Liu, Shengkai Tang, Shengkai Tang, Qichen Jiang Zhiquan Liu, Qichen Jiang Qichen Jiang Qichen Jiang Zhiquan Liu, Zhiquan Liu, Qichen Jiang Zhiquan Liu, Daming Li, Daming Li, Shengkai Tang, Shengkai Tang, Qichen Jiang Qichen Jiang Qichen Jiang Daming Li, Daming Li, Zhiquan Liu, Qichen Jiang Qichen Jiang Qichen Jiang Qichen Jiang Zhiquan Liu, Zhang Tong-qing, Wenyi Zhang, Wenyi Zhang, Zhiquan Liu, Qichen Jiang Qichen Jiang Zhiquan Liu, Qichen Jiang Wenyi Zhang, Zhang Tong-qing, Qichen Jiang Qichen Jiang Zhiquan Liu, Qichen Jiang Qichen Jiang Qichen Jiang Qichen Jiang Qichen Jiang Wenyi Zhang, Qichen Jiang Qichen Jiang Zhiquan Liu, Qichen Jiang Qichen Jiang Zhiquan Liu, Qichen Jiang Qichen Jiang Zhiquan Liu, Zhiquan Liu, Qichen Jiang Zhiquan Liu, Zhiquan Liu, Qichen Jiang Qichen Jiang

Summary

RNA sequencing of Daphnia pulex after 21 days of polystyrene nanoplastic exposure identified 244 differentially expressed genes, with key downregulated genes involved in trehalose metabolism and chitin synthesis and upregulated genes involved in stress response pathways. The transcriptomic analysis reveals metabolic and immune disruption as central mechanisms of chronic nanoplastic toxicity in this keystone freshwater species.

Polymers

Body Systems

Reproductive

Models

Daphnia

Nanoplastic pollution is widespread and persistent across global water systems and can cause a negative effect on aquatic organisms, especially the zooplankter which is the keystone of the food chain. The present study uses RNA sequencing to assess the global change in gene expression caused by 21 days of exposure to 75 nm polystyrene (PS) nanoplastics on Daphnia pulex, a model organism for ecotoxicity. With the threshold value at P value < 0.05 and fold change >2, 244 differentially expressed genes were obtained. Combined with real-time PCR validation of several selected genes, our results indicated that a distinct expression profile of key genes, including downregulated trehalose transporter, trehalose 6-phosphate synthase/phosphatase, chitinase and cathepsin-L as well as upregulated doublesex 1 and doublesex and mab-3 related transcription factor-like protein, contributed to the toxic effects of chronic nanoplastic exposure on Daphnia, such as slowed growth, subdued reproductive ability and reproductive pattern shifting. Our study also showed that chronic exposure to nanoplastic changed the sex ratio of D. pulex neonates. By integrating the gene expression pattern in an important model organism, this study gained insight into the molecular mechanisms of the toxic effect of chronic PS nanoplastic exposure on D. pulex, which may also extend to other nanoplastics or aquatic animals.

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