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4 Single cell RNA-seq samples exposed to nano plastic particles

Zenodo (CERN European Organization for Nuclear Research) 2025 Score: 48 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Vladimir Kovacevic, Biljana Ljujić

Summary

Researchers used microfluidic chip-based single-cell RNA sequencing to profile the transcriptional responses of human peripheral blood immune cells exposed to carboxylated polystyrene nanoparticles of three sizes (40 nm, 200 nm, or a mixture), providing a cell-type-resolved view of nanoplastic effects on the immune system.

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Unraveling the molecular effects of nanoplastics on human blood cells using microfluidic chip-based single-cell RNA sequencing In this study, we exposed human peripheral blood samples to carboxylated polystyrene nanoparticles of different particle sizes and performed single-cell RNA sequencing to examine their effects on gene expression profiles. We leveraged scRNA-seq to investigate the transcriptional consequences of PSNPs of varying sizes: 40 nm (Sample 1), 200 nm (Sample 2), and a combined 40+200 nm mixture (Sample 3) - on human peripheral blood immune cells compared against control sample from the same person, without exposure to PSNPs (Sample 4).

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