We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Toxicological impacts of nanopolystyrene on zebrafish oocyte with insight into the mechanism of action: An expression-based analysis
Summary
Researchers investigated the mechanism by which nanopolystyrene causes toxicity in zebrafish oocytes, finding it triggers oxidative stress, immune disruption, and mitochondrial dysfunction through changes in key gene expression pathways.
Many studies have investigated the negative impacts of microplastics on teleost fishes with very little or no evidence of their mechanism of action. This scenario entreats us to investigate the toxicities of nanopolystyrene in zebrafish oocyte with emphasis on the mechanism of action. In the present study, the cellular levels of mRNA transcripts of different genetic markers (such as: sod, gpx, nrf2, inos, ucp2, and atp6 (redox-sensitive markers); nfkβ, tnfα, il-10, ikβ, gdf9, and bmp15 (immune markers); gadd45, rad51, p53 and bcl2 (DNA damage and apoptotic)) have been quantified by real-time PCR after 6 h of incubation of isolated oocyte with different doses of nanopolystyrene viz. P0 (control i.e. no polystyrene in culture medium), P1 (100 ng/ml), and P2 (400 ng/ml). Results showed that both the treatment concentrations of nanopolystyrene induce oxidative stress with % DPPH = 30.75, 31.61, and 32.43% for P0, P1, and P2, respectively. Increase in oxidative stress in oocytes with increasing doses of nanopolystyrene was also observed in TBARS assay with MDA content 0.12 and 0.21 μM for P1 and P2, respectively as compaired to the control 0.08 μM. This increased oxidative stress can regulate the expression pattern (upregulation/downregulation) of selected genes leading to different toxic effects like - oxidative stress, immunotoxicity, and apoptosis in oocytes, which suggests the impairment of reproductive functions by nanopolystyrene.
Sign in to start a discussion.
More Papers Like This
Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)
Researchers exposed zebrafish embryos to polystyrene nanoplastics at various concentrations and found significant developmental abnormalities including reduced hatching rates and increased malformations. The nanoplastics activated oxidative stress responses and DNA repair pathways, indicating cellular damage during critical early development stages. The study provides mechanistic evidence for how nanoplastic exposure can disrupt normal embryonic development in aquatic organisms.
Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
Researchers exposed zebrafish embryos to polystyrene nanoplastics at various concentrations and measured gene expression changes related to stress, inflammation, and DNA repair. They found dose-dependent activation of oxidative stress and apoptotic pathways at the highest concentration, along with inhibition of the neurotransmitter-related gene acetylcholinesterase and DNA repair genes. The study suggests that nanoplastic exposure at the molecular level may compromise cellular defense mechanisms and neurological function in developing fish.
Impact of polystyrene nanoplastics on apoptosis and inflammation in zebrafish larvae: Insights from reactive oxygen species perspective
Researchers showed that polystyrene nanoplastics accumulate in zebrafish larvae and trigger excessive reactive oxygen species production via NADPH oxidase upregulation, causing mitochondrial dysfunction, apoptosis, and NF-κB-driven inflammation — with inhibiting ROS generation effectively blocking downstream cell death and inflammatory responses.
Polystyrene nanoplastics cause developmental abnormalities, oxidative damage and immune toxicity in early zebrafish development
Zebrafish embryos exposed to polystyrene nanoplastics showed dose-dependent developmental problems including delayed hatching, reduced survival, smaller body size, and the nanoplastics accumulated in critical organs like the eyes, heart, liver, and brain. The particles triggered oxidative stress that damaged cells and activated inflammatory immune responses, demonstrating how nanoplastic contamination in water can cause widespread harm to developing organisms.
From mothers to offspring: Polystyrene nanoplastics create a hidden toxic legacy via mitochondrial dysfunction
Researchers exposed female zebrafish to polystyrene nanoplastics before mating with unexposed males and found that maternal exposure at 100 μg/L reduced offspring hatching success and caused developmental defects in the F1 generation raised in clean water, demonstrating transgenerational toxicity via mitochondrial dysfunction.