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Diverse Perspectives Illuminate the Intestinal Toxicity of Traditional and Biodegradable Agricultural Film Microplastics to Eisenia fetida under Varying Exposure Sequences

Environmental Science & Technology 2025 8 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 63 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Tianyi Cao, Tianyi Cao, Kailun Sun, Kailun Sun, Erkai He, Xinde Cao, Ling Zhao, Xiaoyun Xu, Hao Qiu

Summary

Researchers compared how traditional polyethylene and biodegradable polylactic acid (PLA) microplastics from agricultural films affect earthworm intestines. Regular polyethylene caused more severe gut damage and immune disruption than the biodegradable alternative, though PLA still impaired digestive function. This matters because earthworms are essential for soil health, and damage to them from agricultural plastic film fragments could reduce soil quality and affect the crops humans depend on for food.

The widespread use of plastic agricultural films necessitates a thorough evaluation of environmental risks posed by soil microplastics (MPs). While the intestinal tract is a critical site for MP interactions in soil organisms, current research predominantly focuses on overall physiological responses, overlooking organ-specific toxic mechanisms. To address this gap, we exposed earthworms (Eisenia fetida) to polyethylene (PE) and biodegradable polylactic acid (PLA) MPs sourced from agricultural films at an environmentally realistic concentration of 1.0 g/kg. Incorporating natural earthworm mobility, we designed two exposure scenarios: migration from clean to contaminated soil (scenario A) and vice versa (scenario B). Machine learning-driven image analysis and phenotypic profiling revealed that PE induced more severe intestinal lesions than PLA, adversely affecting intestinal immune functions. Furthermore, PE resulted in greater oxidative damage and significantly activated immune proteins such as melanin and antimicrobial peptides through reprograming immune-related gene and protein pathways. Conversely, PLA predominantly disrupted intestinal digestive and absorptive functions, though the gut microbial community partially mitigated damage through structural and compositional adaptation. Compared with scenario A, earthworms in scenario B exhibited reduced tissue damage, enhanced digestive enzyme activity, and upregulated energy-related metabolites and cell proliferation genes, indicating partial recovery from MP-induced intestinal dysfunction. These findings elucidate the distinct toxicity mechanisms of conventional and biodegradable agricultural MPs on soil organisms, while the scenario-based approach advances risk assessment by aligning experimental design with real-world ecological behaviors.

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