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Meta Analysis Tier 1

Do Added Microplastics, Native Soil Properties, and Prevailing Climatic Conditions Have Consequences for Carbon and Nitrogen Contents in Soil? A Global Data Synthesis of Pot and Greenhouse Studies

This meta-analysis examined how microplastics affect carbon and nitrogen levels in soil, which are key to soil fertility. The results show that certain types of plastics — especially smaller, fiber-shaped particles — can significantly alter soil chemistry, potentially affecting crop growth and soil health.

2024 Environmental Science & Technology 53 citations
Systematic Review Tier 1

Impact of microplastics on the human gut microbiome: a systematic review of microbial composition, diversity, and metabolic disruptions

This systematic review of 12 studies found that microplastics including polyethylene, polystyrene, and PVC induce gut dysbiosis in humans, reducing beneficial bacteria and enriching pathogens. Microplastic exposure also impairs short-chain fatty acid production and modulates immune pathways, contributing to intestinal disease, metabolic syndrome, and chronic inflammation.

2025 BMC Gastroenterology 19 citations
Article Tier 2

Polylactic Acid Micro/Nanoplastic Exposure Induces Male Reproductive Toxicity by Disrupting Spermatogenesis and Mitochondrial Dysfunction in Mice

Even so-called "eco-friendly" biodegradable plastic (polylactic acid, or PLA) was found to cause reproductive harm in male mice. After breaking down in the digestive system, tiny PLA nanoparticles crossed into the testes and damaged sperm quality, mitochondria (the energy producers in cells), and hormone levels. This challenges the assumption that biodegradable plastics are safe and highlights potential risks to male fertility.

2025 ACS Nano 55 citations
Article Tier 2

Polylactic acid microplastics before and after aging induced neurotoxicity in zebrafish by disrupting the microbiota-gut-brain axis

Researchers exposed zebrafish to microplastics made from PLA, a common biodegradable plastic, and found that both new and aged PLA particles caused brain and nerve damage, including sluggish behavior, memory problems, and increased aggression. Aged PLA particles were even more toxic, and the damage appeared to work through disruption of the gut-brain connection, raising concerns about the safety of biodegradable plastics as they break down in water.

2025 Journal of Hazardous Materials 21 citations
Article Tier 2

Incorporation of polylactic acid microplastics into the carbon cycle as a carbon source to remodel the endogenous metabolism of the gut

Researchers discovered that gut bacteria can break down so-called biodegradable PLA microplastics and incorporate the carbon into their own metabolism, fundamentally altering the gut's energy balance. This process reduced beneficial short-chain fatty acids that fuel gut lining cells and caused decreased appetite and weight loss in mice, suggesting that biodegradable plastics may not be as harmless inside the body as assumed.

2025 Proceedings of the National Academy of Sciences 26 citations
Meta Analysis Tier 1

Microplastics and Soil Greenhouse Gas Emissions: A Critical Reflection on Meta-Analyses

This meta-analysis pools data from multiple studies to assess whether microplastics in agricultural soil affect greenhouse gas emissions. The findings reveal that the environmental impact of microplastics extends beyond direct toxicity, as they may alter soil microbial activity in ways that contribute to climate change.

2025 Environmental Science & Technology 5 citations
Article Tier 2

Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease

Researchers found that microplastics worsen chronic obstructive pulmonary disease (COPD) by triggering a chain reaction in lung cells: the plastics damage mitochondria (the cell's energy centers), which produces harmful molecules that activate a self-destructive process called autophagy-dependent ferroptosis. Lung tissue from COPD patients contained significantly higher concentrations of polystyrene microplastics than healthy controls. When scientists blocked this destructive pathway in mice, it reduced the excessive inflammation and prevented COPD flare-ups caused by microplastic exposure.

2025 Autophagy 28 citations
Article Tier 2

The combination of microplastics and glyphosate affects the microbiome of soil inhabitant Enchytraeus crypticus

Researchers tested how microplastics and the common herbicide glyphosate affect soil health when present together. Biodegradable PLA plastic combined with glyphosate had the most damaging effects on both soil bacteria and the gut microbiome of soil worms, worse than conventional PET plastic. These results suggest that using biodegradable plastics alongside pesticides in agriculture may pose greater ecological risks than previously thought.

2025 Journal of Hazardous Materials 21 citations
Article Tier 2

Effects of different microplastic types on soil physicochemical properties, enzyme activities, and bacterial communities

A 230-day experiment tested six types of microplastics in soil and found that each type differently altered soil moisture, chemistry, enzyme activity, and microbial communities. Biodegradable plastic (PHA) caused the most significant disruption to beneficial soil bacteria, which matters for human health because soil microbe changes can affect crop nutrition and food safety.

2024 Ecotoxicology and Environmental Safety 47 citations
Article Tier 2

Oligomer nanoparticle release from polylactic acid plastics catalysed by gut enzymes triggers acute inflammation

Researchers found that polylactic acid (PLA), a popular 'eco-friendly' biodegradable plastic, releases nanoplastic particles when broken down by gut enzymes during digestion. In mice, these PLA fragments accumulated in the liver, intestine, and brain, causing intestinal damage and acute inflammation by interfering with a key immune enzyme, raising important questions about whether biodegradable plastics are truly safer for human health.

2023 Nature Nanotechnology 212 citations
Article Tier 2

Microplastics change soil properties, plant performance, and bacterial communities in salt-affected soils

This study found that microplastics change soil chemistry, plant growth, and bacterial communities in salt-affected soils, with biodegradable polylactic acid plastic having stronger effects than conventional polyethylene. These changes to soil and plant systems are relevant to food safety and human health because they could alter crop quality and nutrient content in agricultural areas affected by both salt and plastic pollution.

2024 Journal of Hazardous Materials 60 citations
Article Tier 2

Comparison of reproductive toxicity between pristine and aged polylactic acid microplastics in Caenorhabditis elegans

This study compared the effects of new versus UV-aged biodegradable PLA microplastics on reproductive health using a worm model, finding that aged particles caused significantly more reproductive damage and DNA injury. The results suggest that biodegradable plastics become more toxic as they weather in the environment, which matters because these aged particles are what organisms, including humans, are most likely to encounter.

2024 Journal of Hazardous Materials 51 citations
Article Tier 2

Plastic food? Energy compensation of zebrafish (Danio rerio) after long-term exposure to polylactic acid biomicroplastics

Zebrafish exposed to biodegradable PLA (polylactic acid) microplastics for 90 days accumulated more plastic in their guts than fish exposed to conventional PET plastic, and suffered more intestinal damage. Although the fish partially compensated by using the PLA breakdown products for energy, the study shows that bio-based plastics still carry meaningful ecological risks for aquatic organisms that can enter our food chain.

2024 Journal of Hazardous Materials 40 citations
Article Tier 2

Antibiotic resistance genes and virulence factors in the plastisphere in wastewater treatment plant effluent: Health risk quantification and driving mechanism interpretation

Researchers found that microplastics in treated wastewater carry significantly more disease-causing bacteria, antibiotic resistance genes, and virulence factors on their surfaces compared to the surrounding water. This means microplastics released from wastewater treatment plants into rivers and lakes could spread antibiotic-resistant infections, posing a direct risk to communities that rely on these water sources.

2024 Water Research 54 citations
Article Tier 2

Biodegradable plastics: mechanisms of degradation and generated bio microplastic impact on soil health

This review explains how biodegradable plastics break down through mechanical, thermal, light, and microbial processes, but warns that they still produce tiny fragments called bio-microplastics during degradation. These bio-microplastic fragments can affect soil health in both positive and negative ways, meaning that switching to biodegradable plastics does not fully eliminate the microplastic problem.

2024 Biodegradation 40 citations
Article Tier 2

Microplastics alter cadmium accumulation in different soil-plant systems: Revealing the crucial roles of soil bacteria and metabolism

A study found that microplastics in soil can change how much cadmium, a toxic heavy metal, is absorbed by food crops, with the effects varying depending on soil type and the amount of plastic present. By altering soil chemistry and bacterial communities, microplastics reshape how pollutants move through farmland and into the food we eat.

2024 Journal of Hazardous Materials 44 citations
Article Tier 2

Unveiling the detrimental effects of polylactic acid microplastics on rice seedlings and soil health

Researchers found that even biodegradable polylactic acid (PLA) microplastics significantly harmed rice plants at high concentrations, reducing root and shoot weight by roughly half and disrupting photosynthesis, while also altering soil enzyme activity and bacterial communities. These findings challenge the assumption that biodegradable plastics are harmless to agriculture and raise questions about their impact on food crops that humans depend on.

2024 Chemosphere 41 citations
Article Tier 2

Comparative Analysis of the Environmental Impact of Biopolymer-Based and Conventional Plastic Packaging in Food Engineering Applications

This review compares plant-based biodegradable packaging materials with traditional plastics like PET and HDPE used in food packaging. While bioplastics produce fewer carbon emissions and break down more easily, they still face cost and performance challenges. The study matters because conventional plastic packaging is a major source of microplastic pollution in the environment.

2025 Al-Mustaqbal Journal of Sustainability in Engineering Sciences 16 citations
Article Tier 2

iMulch: an investigation of the influence of polymers on a terrestrial ecosystem using the example of mulch films used in agriculture

This research project studied how plastic mulch films used in farming break down into microplastics in soil, comparing conventional polyethylene films with biodegradable alternatives. The findings show that both types of mulch release microplastic particles into agricultural soil, though they behave differently in the environment, raising questions about the true sustainability of biodegradable farm plastics.

2025 Environmental Sciences Europe 13 citations
Article Tier 2

Metagenomic analysis reveals soil microbiome responses to microplastics and ZnO nanoparticles in an agricultural soil

Researchers used advanced genetic analysis to show that microplastics and zinc oxide nanoparticles together alter soil microbe communities in ways that disrupt nutrient cycling, including carbon and nitrogen processing. Notably, biodegradable PLA plastic caused more harm to fungal communities than conventional plastics like polyethylene, challenging the assumption that biodegradable plastics are always safer for the environment.

2025 Journal of Hazardous Materials 17 citations
Article Tier 2

Aging behaviors intensify the impacts of microplastics on nitrate bioreduction-driven nitrogen cycling in freshwater sediments

This study found that microplastics that have aged in the environment have stronger effects on nitrogen cycling in lake sediments than fresh microplastics, significantly altering how bacteria process nitrogen. These disruptions to natural nutrient cycles in freshwater systems could affect water quality and the broader food web that ultimately connects to human food sources.

2025 Water Research 18 citations
Article Tier 2

Functionalization of slow-release fertilizers and “passive predation microplastics” mechanism for polylactic acid composites

Researchers developed a biodegradable fertilizer film made from polylactic acid (PLA) and modified lignin that can slowly release nutrients while breaking down naturally in soil, offering an alternative to conventional plastic mulch. The study also explored how plants absorb tiny fragments of bio-based plastics, which is important for understanding whether even biodegradable alternatives still pose risks to food safety.

2025 Journal of Hazardous Materials 10 citations
Article Tier 2

In-field degradation of polybutylene adipate-co-terephthalate (PBAT) films, microplastic formation, and impacts on soil health

A three-year field study found that biodegradable PBAT mulch films actually produced more microplastic particles in soil than conventional polyethylene films, though the biodegradable versions improved soil health and crop yields overall. The majority of microplastics from biodegradable films were very small (under 0.25 mm), which raises questions about whether these tiny fragments pose different risks than larger pieces.

2025 Environmental Research 12 citations
Article Tier 2

Photoaging Promotes Toxic Micro/Nanoplastics Release from PLA/PBAT Biodegradable Plastic in Gastrointestinal Condition

Researchers found that when biodegradable plastics made from PLA/PBAT are aged by sunlight and then exposed to digestive conditions, they release significantly more micro- and nanoplastic particles than non-aged versions. These released particles showed toxicity to intestinal cells, raising important questions about the safety of biodegradable food packaging as it weathers before disposal.

2025 Environment & Health 18 citations