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61,005 resultsShowing papers similar to Detection of polystyrene nanoplastics in biological samples based on the solvatochromic properties of Nile red: application in Hydra attenuata exposed to nanoplastics
ClearInnovative application of Nile Red (NR)-based dye for direct detection of micro and nanoplastics (MNPs) in diverse aquatic environments
Researchers developed a method using Nile Red fluorescent dye in n-heptane to directly detect micro- and nanoplastics in diverse water types without prior extraction or processing, achieving sensitive detection of polystyrene, PET, and latex microspheres. The approach offers significant time savings compared to conventional detection methods.
Nile Red staining for detecting microplastics in biota: Preliminary evidence
Nile Red fluorescent staining was tested for identifying microplastics in biological tissue samples, finding that it successfully highlighted plastic particles in fish guts and bivalve tissues with minimal interference from digested organic residues, supporting its use as a quick screening tool before confirmatory spectroscopy.
A Simple Spectral Method for Nanoplastic Identification and Characterisation
Researchers developed a fluorescence mapping method using Nile Red staining to locate, quantify, and identify polystyrene and polyethylene terephthalate nanoplastics down to 60 nm in size, overcoming the diffraction limits of conventional spectroscopy. Verification by scanning electron microscopy confirmed the technique can resolve individual nanoplastics of different types and sizes in complex real-world samples containing contaminant and additive nanoparticles.
Detection of polystyrene nanoplastics in biological tissues with a fluorescent molecular rotor probe
A fluorescent dye was developed to detect polystyrene nanoplastics in biological tissues, offering a simpler alternative to existing methods. This tool could help scientists better understand how nanoplastics spread through living organisms and accumulate in organs.
A colorimetric detection of polystyrene nanoplastics with gold nanoparticles in the aqueous phase
Researchers developed a colorimetric detection method using gold nanoparticles to identify polystyrene nanoplastics in water, providing a simpler and more sensitive alternative to traditional spectroscopy methods for detecting nanoplastics that are too small for conventional microplastic analysis.
Rapid detection of nanoplastics and small microplastics by Nile-Red staining and flow cytometry
Researchers developed a rapid method for detecting nanoplastics and small microplastics by combining Nile-Red fluorescent staining with flow cytometry. The technique can quantify plastic particles in the 0.6 to 15 micrometer range in just 90 seconds, which is hundreds of times faster than conventional spectroscopic methods. The approach showed high detection efficiency for polyethylene, polyvinylchloride, and polystyrene, offering a practical tool for environmental nanoplastic monitoring.
Differentiation of different subtypes of polystyrene microplastics using Nile red
Researchers used the fluorescent dye Nile red with optimized concentration and temperature conditions to differentiate five subtypes of polystyrene microplastics—including high-impact, expandable, carboxylated, and aminated forms—based on differences in dye uptake efficiency.
Isolation and Quantification of Polystyrene Nanoplastics in Tissues by Low Pressure Size Exclusion Chromatography
Researchers developed a size exclusion chromatography method to isolate and quantify polystyrene nanoplastics (50 and 100 nm) from biological tissue homogenates using fluorescent dye detection and salting-out concentration. The method was validated in tissue from clams from a high-traffic harbor and freshwater mussels downstream of an urban area.
Preparation of biological samples for microplastic identification by Nile Red
This study optimized sample preparation procedures for Nile Red staining of microplastics in biological matrices, identifying interfering substances and developing pre-treatment steps that improve fluorescence staining specificity.
Identification and quantification of microplastics using Nile Red staining
Researchers tested Nile Red staining as a method for identifying and quantifying microplastics in environmental samples, finding it useful for rapid screening but noting limitations in distinguishing plastics from non-plastic particles.
Nile red staining for rapid screening of plastic-suspect particles in edible seafood tissues
Researchers developed a faster method for detecting microplastics in seafood using a fluorescent dye called Nile red, which makes plastic particles glow under special light. The technique correctly identified about 72% of suspected plastic particles in fish and shrimp samples, making it useful for large-scale screening. Faster detection methods like this are important for monitoring how much microplastic contamination is present in the seafood people eat.
Determination of polystyrene nanospheres and other nanoplastics in water via binding with organic dyes by capillary electrophoresis with laser-induced fluorescence detection
Researchers developed a new capillary electrophoresis method using fluorescent organic dyes to quantitatively detect polystyrene nanospheres and other nanoplastics in water samples, successfully differentiating them from microplastics and colloidal particles.
Analysis of selective fluorescence for the characterization of microplastic fibers: Use of a Nile Red-based analytical method to compare between natural and synthetic fibers
Researchers developed a Nile Red fluorescence method to distinguish natural from synthetic microplastic fibers, demonstrating that selective fluorescence staining combined with spectral analysis can improve identification accuracy for fiber-type microplastics across different environments.
Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost- and time-effective technique
Researchers developed a semi-automated, cost-effective method for microplastic detection using Nile red fluorescent staining, showing it can significantly reduce the time and expense of identifying microplastics compared to traditional spectroscopic approaches.
A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red
Researchers developed a rapid fluorescent screening method using Nile Red dye to detect and quantify microplastics in environmental samples, finding it significantly faster than conventional methods while maintaining reasonable accuracy.
A novel extraction protocol of nano-polystyrene from biological samples
Researchers developed a diatomite-based extraction protocol for isolating nano-polystyrene from biological samples, providing a method to quantify nanoplastics in tissues that had previously lacked reliable analytical tools.
Development of a method for the detection of polystyrene microplastics in paraffin-embedded histological sections
Researchers developed a method for detecting polystyrene microplastics in paraffin-embedded tissue samples, addressing a key constraint in assessing microplastic exposure in marine animals used in laboratory toxicity bioassays.
Exploring Nile Red staining as an analytical tool for surface-oxidized microplastics
Scientists evaluated Nile Red, a fluorescent dye commonly used to detect microplastics, and found it works differently depending on whether microplastics have been weathered by the environment. Surface oxidation from aging in the environment changes how well the dye sticks to plastics, which means current detection methods may be undercounting weathered microplastics in environmental samples.
Nile Red lifetime reveals microplastic identity
Researchers developed a fluorescence lifetime imaging approach using the dye Nile Red that can distinguish microplastic particles from biological and mineral debris based on their distinct fluorescence lifetimes, offering faster and more specific microplastic identification than conventional methods.
Detection, biophysical effects, and toxicity of polystyrene nanoparticles to the cnidarian Hydra attenuata
Researchers exposed the freshwater cnidarian Hydra attenuata to 50- and 100-nm polystyrene nanoparticles and found size-dependent bioaccumulation, morphological damage, lipid peroxidation, and intracellular liquid crystal formation — with 100-nm particles more toxic per particle count, suggesting that lipid mobilization and organized intracellular structures may represent a novel nanoplastic toxicity mechanism in simple aquatic animals.
Fluorescent technique to detect microplastics in a natural matrix using Methylene blue and Nile red
Researchers tested methylene blue fluorescent staining as a low-cost technique for detecting microplastics in complex natural matrices such as sediment and biological tissue, finding the method provided sufficient contrast for visual identification without requiring expensive spectroscopic equipment.
Characterization of Nile Red-Stained Microplastics through Fluorescence Spectroscopy
Researchers developed an improved method for characterizing microplastics using Nile Red fluorescent staining combined with fluorescence spectroscopy. They found that different plastic polymers produce distinct fluorescent signatures when stained, enabling more reliable identification of plastic types. The technique offers a faster and more affordable alternative to traditional microplastic detection methods, which could help scale up environmental monitoring efforts.
The potential of fluorescent dyes—comparative study of Nile red and three derivatives for the detection of microplastics
Researchers compared Nile red and three newly developed fluorescent dye derivatives for staining microplastics, finding that the derivatives achieved greater selectivity for plastic particles and more intense fluorescence than standard Nile red, improving detection sensitivity.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.