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20 resultsShowing papers similar to Improvement of biodegradation of PET microplastics with whole-cell biocatalyst by interface activation reinforcement
ClearInterfacial engineering-based colonization of biofilms on polyethylene terephthalate (PET) surfaces: Implications for whole-cell biodegradation of microplastics
This study applied interfacial engineering to promote biofilm colonization on polyethylene terephthalate (PET) surfaces to facilitate enzymatic depolymerization under mild conditions. The engineered biofilm approach enabled efficient PET biodegradation without requiring harsh alkaline conditions or high temperatures, advancing practical plastic bioremediation.
Biodegradation of Microplastic Derived from Poly(ethylene terephthalate) with Bacterial Whole-Cell Biocatalysts
Engineered bacterial whole-cell biocatalysts were used to biodegrade PET microplastics under alkaline conditions, with the strain using PET as a sole carbon source and producing monomers that did not accumulate due to continuous cellular metabolism. The study demonstrates a combined enzymatic-microbial approach that overcomes product inhibition in enzymatic PET degradation.
Targeted aggregation of PETase towards surface of Stenotrophomonas pavanii for degradation of PET microplastics
Researchers developed a strategy to target PETase enzyme to the surface of Stenotrophomonas pavanii bacteria, improving the efficiency of in-situ PET microplastic degradation. Surface-displayed PETase showed significantly enhanced PET hydrolysis compared to free enzyme, offering a practical approach to microbial degradation of dispersed PET microplastics in environmental settings.
Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase
Researchers engineered bacteria to display a PET-degrading enzyme on their surface, creating a reusable biocatalyst capable of breaking down polyethylene terephthalate plastics. The system worked under various conditions, remained stable for at least 30 days, and could even degrade PET microplastics in wastewater and highly crystalline consumer plastic waste. This biological approach offers a promising environmentally friendly alternative for plastic recycling and waste treatment.
Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin
Researchers engineered yeast cells to display both a PET-degrading enzyme (PETase) and a sticky protein (hydrophobin) on their surface simultaneously, dramatically improving the breakdown of highly crystalline PET plastic — achieving a 329-fold increase in degradation rate compared to the purified enzyme alone. This whole-cell biocatalyst approach could make enzymatic plastic recycling far more practical and efficient.
The proliferation and colonization of functional bacteria on amorphous polyethylene terephthalate: Key role of ultraviolet irradiation and nonionic surfactant polysorbate 80 addition
Researchers showed for the first time that UV irradiation and the surfactant Tween-80 act synergistically to promote bacterial colonization of amorphous PET plastic: UV creates surface attachment sites while Tween-80 boosts bacterial proliferation and surface hydrophobicity, together setting the stage for subsequent biodegradation.
Acceleration a yeast-based biodegradation process of polyethylene terephthalate microplastics by Tween 20: Efficiency, by-product analysis, and metabolic pathway Prediction
Researchers isolated a new yeast strain capable of degrading polyethylene terephthalate microplastics and found that adding the surfactant Tween 20 significantly accelerated the biodegradation process. The yeast changed the microplastic surface charge and reduced particle size, with Tween 20 enhancing the breakdown efficiency. The study suggests that surfactant-assisted biological approaches may offer a promising avenue for addressing PET microplastic pollution.
Development and characterization of a bacterial enzyme cascade reaction system for efficient and stable PET degradation
Scientists engineered a bacterial system that displays plastic-degrading enzymes on the cell surface to efficiently break down PET plastic, achieving a 23% degradation rate of microplastics within 7 days. The system uses E. coli bacteria with specially designed protein fibers that both grip and digest PET fragments. This biotechnology approach could eventually help address the growing problem of microplastic pollution in water and soil environments.
Engineered Vibrio natriegens as a living biocatalyst for in-situ biodegradation of microplastics in seawater
Researchers engineered the fast-growing marine bacterium Vibrio natriegens to display PETase enzymes on its outer membrane, creating a living biocatalyst that degrades PET microplastics directly in seawater conditions, outperforming comparable E. coli-based systems in both growth rate and hydrolytic activity. This halophilic whole-cell approach addresses a key gap in bioremediation — most PETase studies use freshwater organisms that cannot survive the salinity of marine environments where plastic pollution is most severe.
Biodegradation of Poly(Ethylene Terephthalate) Microplastics by Baceterial Communities From Activated Sludge
Scientists isolated bacteria from wastewater treatment sludge that can biodegrade PET plastic, used in plastic bottles and food packaging. The bacteria broke down PET microplastics over a 60-day period, pointing toward a potential biological tool for removing plastic contamination from water treatment systems.
Biodegradation of Poly(Ethylene Terephthalate) Microplastics by Baceterial Communities From Activated Sludge
Scientists isolated bacteria from wastewater treatment sludge that can biodegrade PET plastic, used in plastic bottles and food packaging. The bacteria broke down PET microplastics over a 60-day period, pointing toward a potential biological tool for removing plastic contamination from water treatment systems.
Marine hydrocarbon-degrading bacteria breakdown poly(ethylene terephthalate) (PET)
Scientists used microcosm studies to investigate whether marine hydrocarbon-degrading bacteria can break down PET plastic, finding that specific bacterial strains could colonize and degrade PET surfaces, offering insights into natural plastic biodegradation in the ocean.
Degradation of PET Plastics by Wastewater Bacteria Engineered via Conjugation
Researchers demonstrated a proof-of-concept approach for reducing PET microplastic pollution in wastewater by engineering bacteria in situ via conjugation to express PET-degrading enzymes. The study used a broad-host-range conjugative plasmid to transfer PET hydrolase genes into native wastewater bacterial communities.
Modulating biofilm can potentiate activity of novel plastic-degrading enzymes
Researchers discovered two new enzymes capable of breaking down PET plastic (the kind used in plastic bottles) and found that boosting a bacterium's ability to form a biofilm — a sticky coating that helps bacteria cling to surfaces — significantly increased how fast the enzymes could degrade plastic. This biofilm strategy could help accelerate the development of biological plastic-recycling systems for waste that would otherwise end up in landfills.
An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics
This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.
Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis
This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.
Enhanced degradation of polyethylene terephthalate (PET) microplastics by an engineered Stenotrophomonas pavanii in the presence of biofilm
Scientists engineered a biofilm-forming bacterium to break down PET microplastics (the type found in water bottles and food containers) at room temperature. The engineered bacteria achieved significant PET degradation over 30 days and also worked on other polyester plastics, offering a potential biological solution for cleaning up microplastic pollution in water environments.
Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Researchers are working to accelerate the biodegradation of PET plastic by Piscinibacter sakaiensis, a bacterium that naturally evolved to consume this common type of plastic. Using bioactivity screens and degradation tests, they identified a small number of biochemical conditions that more than doubled the PET biodegradation rate. The work provides a foundation for developing a fermentation process that could help address PET plastic pollution at scale.
Current Knowledge on Polyethylene Terephthalate Degradation by Genetically Modified Microorganisms
This review covers genetically modified microorganisms engineered to degrade polyethylene terephthalate, examining how bioengineering of enzymes such as PETase and enhanced expression systems can overcome the low biodegradation rates of wild-type microorganisms toward this ubiquitous plastic.
Bioengineering Comamonas testosteroni CNB-1: a robust whole-cell biocatalyst for efficient PET microplastic degradation
This study engineered Comamonas testosteroni CNB-1 as a whole-cell biocatalyst for degrading PET microplastics in biological wastewater treatment, addressing the accumulation of these particles in sewage sludge. The engineered bacterium demonstrated efficient PET degradation, offering a biotechnological solution to a pressing wastewater treatment challenge.