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Improvement of biodegradation of PET microplastics with whole-cell biocatalyst by interface activation reinforcement
Summary
Researchers developed a whole-cell biocatalysis strategy using alkali-resistant bacteria combined with surfactant-mediated interfacial activation to improve the biodegradation of PET microplastics, finding that Tween 20 most effectively enhanced the bio-interfacial activity between bacterial enzymes and the hydrophobic PET surface, leading to improved hydrolysis rates.
Polyethylene terephthalate (PET) is an important basic polymer, which was used widely in variety of fields. Due to its high crystallinity, compact structure and strong surface hydrophobicity, PET has prominent resistance to biodegradation. In recent years, microplastics, especially polyethylene terephthalate (PET) microplastics, was considered as serious threaten to ecosystems. In this study, alkali-resistant bacteria were used as whole-cell catalysts to try to improve the biodegradation of PET microplastics by increasing the bio-interfacial activity of the polymer substrate. Surfactants were applicated to enhance interfacial activation of enzyme and PET interactions. And an integrated strategy was constructed based on alkali resistant bacteria to catalysis the hydrolysis of PET. The results showed that Tween 20 had the most obvious promoting effect among the four interfacial biocatalysts on biological-chemical combined hydrolysis of PET microplastics with whole-cell biocatalysts in alkaline environment. Obvious etching and fracture were observed on the PET fibre surface after biodegradation in presence of surfactant. The weight loss rate of PET substrate can reach 11.04% after 5 days of biodegradation. Thus, this research provides a promising method for efficient degradation of PET microplastics.
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