Papers

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Article Tier 2

Polystyrene (nano)microplastics cause size-dependent neurotoxicity, oxidative damage and other adverse effects inCaenorhabditis elegans

Researchers found that polystyrene micro- and nanoplastics cause neurotoxicity and oxidative damage in the model organism C. elegans, with effects varying by particle size. Smaller nanoscale particles tended to cause more severe toxic responses than larger microplastic particles. The study highlights that the size of plastic particles is an important factor in determining how harmful they are to living organisms.

2018 Environmental Science Nano 422 citations
Article Tier 2

Polystyrene nanoplastics modulate neurite length in a size-specific manner

Researchers exposed primary neurons to polystyrene nanoplastics of three different sizes (50, 100, and 250 nm) at low concentrations to evaluate effects on brain cell development. The study found that nanoplastics modulate neurite length in a size-specific manner, suggesting that even short, low-dose exposures to plastic nanoparticles may affect neuronal growth and connectivity.

2026 NanoImpact
Article Tier 2

Mechanisms Underlying the Size-Dependent Neurotoxicity of Polystyrene Nanoplastics in Zebrafish

Scientists discovered that smaller nanoplastics cause more severe brain and nerve damage in zebrafish than larger ones, and identified the molecular pathways behind this size-dependent toxicity. The smaller particles more easily crossed biological barriers and triggered greater oxidative stress and inflammation in the nervous system, which is important for understanding potential neurological risks of nanoplastic exposure.

2025 Environmental Science & Technology 14 citations
Article Tier 2

Neurotoxic potential of polystyrene nanoplastics in primary cells originating from mouse brain

Researchers exposed three types of primary mouse brain cells to 100 nm polystyrene nanoplastics and found that neurons underwent apoptosis while astrocytes survived but developed reactive astrocytosis with elevated inflammatory markers, suggesting that neuronal vulnerability to nanoplastic accumulation may be amplified by astrocyte-driven neuroinflammation.

2020 NeuroToxicology 118 citations
Article Tier 2

Are all nanoplastics equally neurotoxic? Influence of size and surface functionalization on the toxicity of polystyrene nanoplastics in human neuronal cells

Researchers tested four types of polystyrene nanoplastics on human neuronal cells and found that toxicity varied dramatically depending on particle surface chemistry. Particles with amine surface groups were the most harmful, significantly reducing cell survival and causing visible damage to cell structures, while unmodified particles showed minimal toxicity, suggesting that surface properties matter as much as size when assessing nanoplastic risks.

2025 Environmental Pollution 2 citations
Article Tier 2

Neurotoxicity of polystyrene nanoplastics with different particle sizes at environment-related concentrations on early zebrafish embryos

Researchers exposed zebrafish embryos to polystyrene nanoplastics of different sizes at concentrations found in the environment and observed significant brain damage. The nanoplastics caused loss of neurons, shortened nerve fibers, and disrupted brain signaling systems that control behavior. Smaller nanoplastics caused the most severe damage because they could pass through protective barriers more easily, suggesting that the tiniest plastic particles pose the greatest risk to brain development.

2023 The Science of The Total Environment 99 citations
Article Tier 2

Size-dependent internalization of polystyrene microplastics as a key factor in macrophages and systemic toxicity

Researchers systematically tested how the size of polystyrene microplastics affects their uptake and toxicity in immune cells and mice. Smaller particles (0.5 micrometers) were taken up much more readily by immune cells and caused more damage, including mitochondrial dysfunction and cell death, compared to larger 5-micrometer particles. In living mice, smaller microplastics accumulated more in organs and caused broader changes in blood and metabolic markers, confirming that particle size is a key factor in microplastic toxicity.

2025 Journal of Hazardous Materials 24 citations
Article Tier 2

Analysis of Biodistribution and in vivo Toxicity of Varying Sized Polystyrene Micro and Nanoplastics in Mice

This study found that smaller plastic particles spread more widely through the bodies of mice and caused more organ damage than larger ones, particularly in the liver, kidneys, and heart. Nanoplastics (under 1 micrometer) were especially concerning because they crossed biological barriers more easily than microplastics. The results suggest that the tiniest plastic particles in our environment may pose the greatest health risks.

2024 International Journal of Nanomedicine 36 citations
Article Tier 2

Effects of acute exposure to polystyrene micro and nanoplastics, alone and in combination with the organophosphorus pesticide glyphosate, on the central nervous system of male and female rats

Researchers examined the acute neurotoxic effects of polystyrene micro- and nanoplastics, alone and combined with the pesticide glyphosate, in male and female rats. The study found that both particle sizes induced significant neurotoxicity within 24 hours, with 1-micrometer particles causing more pronounced effects, females showing greater vulnerability, and the hippocampus emerging as the most affected brain region, especially under combined exposure.

2026 Ecotoxicology and Environmental Safety
Article Tier 2

Cytotoxicity of amine-modified polystyrene MPs and NPs on neural stem cells cultured from mouse subventricular zone

Researchers tested the effects of polystyrene microplastics and nanoplastics with a positive surface charge on neural stem cells from mouse brains. Both sizes of particles reduced cell survival, but nanoplastics were significantly more toxic at lower concentrations, causing cell death and preventing stem cells from developing into mature brain cells. These findings suggest that nanoplastics that reach the brain could potentially harm the nervous system's ability to repair and maintain itself.

2024 Heliyon 14 citations
Article Tier 2

Evaluation of size-dependent uptake, transport and cytotoxicity of polystyrene microplastic in a blood-brain barrier (BBB) model

Using a lab model of the blood-brain barrier, researchers found that smaller microplastics (0.2 micrometers) crossed into brain tissue far more readily than larger ones, increasing barrier permeability by up to 27 times after 72 hours. This suggests that the tiniest microplastics may pose the greatest risk to brain health, especially when inflammation is already present.

2024 Nano Convergence 46 citations
Article Tier 2

Human neurons are susceptible to the internalization of small-sized nanoplastics

Researchers studied how human neurons take up nanoplastics and found that the cells readily absorbed 50-nanometer polystyrene particles through specific cellular pathways. The nanoplastics accumulated in cell compartments and, at higher concentrations, triggered oxidative stress and reduced cell survival. The study provides evidence that very small plastic particles can enter human brain cells, raising concerns about potential neurological effects of nanoplastic exposure.

2025 Environmental Toxicology and Pharmacology 4 citations
Article Tier 2

Differential cascading cellular and subcellular toxicity induced by two sizes of nanoplastics

Researchers used time-resolved flow cytometry and confocal imaging to compare the cellular and subcellular toxicity of two sizes of amine-modified polystyrene nanoplastics (100 nm and 1000 nm) on zebrafish cell lines. The study found that the two sizes produced contrasting patterns of cytotoxicity, with different mechanisms of cellular uptake and intracellular distribution. The findings suggest that nanoplastic size is a critical factor in determining the type and severity of cellular damage.

2022 The Science of The Total Environment 48 citations
Article Tier 2

Polystyrene nanoplastics induced size-dependent developmental and neurobehavioral toxicities in embryonic and juvenile zebrafish

Researchers exposed zebrafish embryos and juveniles to polystyrene nanoplastics of three different sizes and found that all sizes crossed into the brain, eyes, and other organs. Smaller particles tended to cause different types of damage than larger ones, including changes in brain development and behavior. This size-dependent toxicity is relevant to human health because we are exposed to a wide range of nanoplastic sizes through food and water.

2024 Aquatic Toxicology 34 citations
Article Tier 2

Correlation between cellular uptake and cytotoxicity of polystyrene micro/nanoplastics in HeLa cells: A size-dependent matter

Researchers tested polystyrene particles of various sizes on human cells and found that only the smallest nanoplastics, those under about 25 nanometers in radius, could enter cells and cause toxic effects. Larger microplastic particles did not penetrate the cell membrane and showed no toxicity even at very high concentrations. The study provides a clear explanation for why smaller plastic particles tend to be more harmful, directly linking cell entry to cellular damage.

2023 PLoS ONE 29 citations
Article Tier 2

Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae

Researchers exposed zebrafish larvae to polystyrene nanoparticles of two sizes (50 nm and 100 nm) to assess size-dependent neurotoxic effects. The study found that 50 nm particles circulated in blood vessels and accumulated in the brain, inducing abnormal behavior and disrupting dopaminergic metabolites, while 100 nm particles had distinct but less pronounced neurological effects.

2022 International Journal of Molecular Sciences 42 citations
Article Tier 2

Bioaccumulation of differently-sized polystyrene nanoplastics by human lung and intestine cells

Researchers examined how human lung and intestine cells take up polystyrene nanoplastics of different sizes, finding that smaller particles were internalized in greater numbers but at lower total mass compared to larger ones. When compared on a surface area basis, the uptake rates were similar across sizes, suggesting that surface interactions with cell membranes play a key role. The findings indicate that particle size is an important factor to consider when evaluating the health risks of nanoplastic exposure.

2022 Journal of Hazardous Materials 86 citations
Article Tier 2

Size-dependent and tissue specific accumulation of polystyrene microplastics and nanoplastics in zebrafish

Researchers tracked size-dependent accumulation of polystyrene micro- and nanoplastics in multiple zebrafish tissues, finding that smaller particles distributed more broadly throughout the body compared to larger ones. Nanoplastics showed greater systemic distribution including into brain and reproductive tissues, raising concerns about size-dependent health risks.

2025 Aquatic Toxicology
Article Tier 2

Cellular interactions with polystyrene nanoplastics—The role of particle size and protein corona

Researchers investigated how polystyrene nanoplastics interact with mammalian cells, finding that particle size and the protein corona that forms around particles in biological fluids strongly influence cellular uptake and toxicity. Smaller nanoplastics penetrated cell membranes more readily and caused greater disruption, suggesting that the tiniest plastic particles may pose the greatest biological risk.

2021 Biointerphases 61 citations
Article Tier 2

DistinctEffects between Polystyrene Micro- and Nanoplastics:Exacerbation of Adverse Outcomes in Inflammatory Bowel Disease-likeZebrafish and Mice

Researchers compared the effects of polystyrene micro- and nanoplastics on a biological system, finding that nanoplastics caused more severe adverse effects than microplastics at equivalent mass doses, likely due to greater surface area and cellular penetration capacity.

2025 Figshare
Article Tier 2

Cellular internalization and release of polystyrene microplastics and nanoplastics

Scientists studied how polystyrene plastic particles of different sizes enter and exit living cells. They found that particles 50 and 500 nanometers in size can penetrate cell membranes and get taken up through multiple pathways, while 5-micrometer particles are too large to enter cells. This research helps explain why smaller nanoplastics may be more harmful to human health, as they can more easily get inside our cells and accumulate there.

2021 The Science of The Total Environment 373 citations
Article Tier 2

Crossing barriers – tracking micro- and nanoplastic pathways into the human brain

Researchers tracked potential pathways by which micro- and nanoplastics may enter the human brain, examining both in vitro cell models and post-mortem brain tissue. They found that human monocytes rapidly internalized polystyrene particles into endocytic vesicles and mitochondria, and detected plastic particles in brain tissue samples, providing evidence that nanoplastics may be capable of crossing brain barriers.

2026
Article Tier 2

Neurotoxic effects of polystyrene nanoplastics on memory and microglial activation: Insights from in vivo and in vitro studies

In a mouse study, tiny nanoplastics (30-50 nanometers) that were swallowed reached the brain and caused memory problems by activating the brain's immune cells, called microglia, which triggered inflammation. This is concerning because it shows that nanoplastics small enough to be found in everyday products like cosmetics could cross into the brain and impair cognitive function.

2024 The Science of The Total Environment 83 citations
Article Tier 2

Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models

Researchers tested the effects of two sizes of polystyrene nanoplastics on fish cell lines from both freshwater and marine species. They found that smaller 20-nanometer particles were significantly more toxic than larger 80-nanometer ones, causing cell death through apoptosis and disrupting multiple biological pathways. The study provides evidence that nanoplastic size is a key factor in determining toxicity to aquatic organisms.

2024 The Science of The Total Environment 9 citations