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Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae

International Journal of Molecular Sciences 2022 42 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 55 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Kyu-Seok Hwang, Yuji Son, Seong Soon Kim, Dae‐Seop Shin, So Hee Lim, Jung Yoon Yang, Ha Neul Jeong, Byung Hoi Lee, Myung Ae Bae

Summary

Researchers exposed zebrafish larvae to polystyrene nanoparticles of two sizes (50 nm and 100 nm) to assess size-dependent neurotoxic effects. The study found that 50 nm particles circulated in blood vessels and accumulated in the brain, inducing abnormal behavior and disrupting dopaminergic metabolites, while 100 nm particles had distinct but less pronounced neurological effects.

Microplastics, small pieces of plastic derived from polystyrene, have recently become an ecological hazard due to their toxicity and widespread occurrence in aquatic ecosystems. In this study, we exposed zebrafish larvae to two types of fluorescent polystyrene nanoparticles (PS-NPs) to identify their size-dependent effects. PS-NPs of 50 nm, unlike 100 nm PS-NPs, were found to circulate in the blood vessels and accumulate in the brains of zebrafish larvae. Behavioral and electroencephalogram (EEG) analysis showed that 50 nm PS-NPs induce abnormal behavioral patterns and changes in EEG power spectral densities in zebrafish larvae. In addition, the quantification of endogenous neurochemicals in zebrafish larvae showed that 50 nm PS-NPs disturb dopaminergic metabolites, whereas 100 nm PS-NPs do not. Finally, we assessed the effect of PS-NPs on the permeability of the blood-brain barrier (BBB) using a microfluidic system. The results revealed that 50 nm PS-NPs have high BBB penetration compared with 100 nm PS-NPs. Taken together, we concluded that small nanoparticles disturb the nervous system, especially dopaminergic metabolites.

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