Papers

61,005 results
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Article Tier 2

Ecological risks of combination of multiple pollutants at environmentally relevant concentrations: Insights from the changes in life history traits, gut microbiota, and transcriptomic responses in Daphnia magna

Researchers exposed Daphnia magna to a combination of 11 pollutants including microplastics, antibiotics, and heavy metals at environmentally relevant ng/L–μg/L concentrations and found significant reductions in heart rate, reproduction, and lifespan, plus gut microbiota and transcriptomic changes — effects that single-pollutant studies would not predict.

2025 Resources Environment and Sustainability
Article Tier 2

Combined effect of microplastics and tire particles on Daphnia magna: Insights from physiological and transcriptomic responses

Researchers investigated the combined effects of microplastics and tire particles on the water flea Daphnia magna, finding that the mixture triggered significant oxidative stress at environmentally relevant concentrations. Transcriptomic analysis revealed upregulation of antioxidant and metabolic stress genes, while energy reserves like glycogen were affected. The study suggests that co-exposure to these common freshwater pollutants may pose greater ecological risks than either particle type alone.

2025 Environmental Pollution 1 citations
Article Tier 2

Impacts of microplastics and pesticides on Daphnia

Researchers investigated the combined and individual impacts of microplastics and pesticides on Daphnia magna, a model crustacean widely used in freshwater ecotoxicology, to assess how these co-occurring pollutants affect aquatic ecosystem health. The study examined survival, reproduction, and physiological responses in D. magna exposed to varying concentrations of both stressors under controlled conditions.

2025 Fisher Digital Publications (St. John Fisher College)
Article Tier 2

Co-exposure of microplastics and 2-methoxy-1,4-naphthoquinone affects Daphnia magna depending on the developmental stage

Researchers studied the combined effects of microplastics and the plant-derived compound 2-methoxy-1,4-naphthoquinone on Daphnia magna, which represents a freshwater ecosystem stress scenario. Co-exposure produced effects that differed from either stressor alone, highlighting the importance of studying pollutant mixtures.

2024 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Metabolomic analysis of combined exposure to microplastics and methylmercury in the brackish water flea Diaphanosoma celebensis

Combined exposure of the brackish water flea Diaphanosoma celebensis to microplastics and methylmercury produced metabolomic disruptions greater than either pollutant alone, with the combination altering amino acid metabolism, energy pathways, and oxidative stress markers. The study provides molecular-level evidence that microplastic-mercury co-contamination poses synergistic risks to aquatic invertebrates.

2022 Environmental Geochemistry and Health 14 citations
Article Tier 2

Combined Effect of Polystyrene Particles and Copper Ions on the Vital Parameters of Daphnia Magna in a Series of Generations

Researchers studied the combined effects of polystyrene microplastics and copper ions on Daphnia magna over four generations using both short-term and continuous exposure designs. Toxicity was greater under combined exposure and increased across generations, suggesting cumulative intergenerational harm from mixed pollutant stress.

2025 Transactions of Papanin Institute for Biology of Inland Waters RAS
Article Tier 2

Effects of combined nutrient and pesticide exposure on algal biomass and Daphnia magna abundance

Researchers investigated the individual and combined effects of nutrients and pesticides on Daphnia magna abundance and algal biomass under controlled conditions, examining how agricultural inputs — fertilizers driving eutrophication and pesticides causing direct toxicity — interact to affect freshwater biodiversity.

2022 Research Square (Research Square) 2 citations
Review Tier 2

Ecotoxicology of microplastics in Daphnia: A review focusing on microplastic properties and multiscale attributes of Daphnia

This review synthesizes research on how microplastics affect Daphnia, a key organism in aquatic food webs, across individual, population, and community levels. Researchers found that the toxicity of microplastics to Daphnia depends heavily on the physical and chemical properties of the particles, and that combined exposure with other pollutants can produce more severe effects. The study highlights Daphnia as an important indicator species for understanding how microplastic pollution cascades through aquatic ecosystems.

2022 Ecotoxicology and Environmental Safety 71 citations
Article Tier 2

Effects of Exposure to Cadmium, Microplastics, and Their Mixture on Survival, Growth, Feeding, and Life History of Daphnia magna

Researchers examined how polyethylene microplastics altered cadmium toxicity to Daphnia magna, finding that microplastic co-exposure modified cadmium bioavailability and affected survival, growth, feeding rates, and reproductive outcomes in this ecologically important species.

2023 Environmental Toxicology and Chemistry 9 citations
Article Tier 2

Action of Surfactants in Driving Ecotoxicity of Microplastic-Nano Metal Oxides Mixtures: A Case Study on Daphnia magna under Different Nutritional Conditions

This study tested how surfactants (found in detergents and cleaning products) interact with microplastics and metal oxide nanoparticles to affect the toxicity of these combined pollutants on water fleas (Daphnia magna). Surfactants increased the toxicity of microplastic-nanoparticle mixtures, and the effect varied with the age and nutritional status of the test organisms. This highlights how the complex real-world mixture of pollutants in waterways can be more harmful than any single contaminant alone.

2021 IntechOpen eBooks 2 citations
Article Tier 2

Multispecies assemblages and multiple stressors: Synthesizing the state of experimental research in freshwaters

This synthesis of experimental freshwater research on multispecies assemblages under multiple stressors found that most studies used only two species and two stressors, revealing important knowledge gaps about how real-world complexity of biodiversity loss and combined pollution affects freshwater ecosystem function.

2023 Wiley Interdisciplinary Reviews Water 18 citations
Article Tier 2

Study of the toxicological effects of emerging contaminants on Daphnia similis associating polyethylene microplastics with the agrochemical imidacloprid.

Brazilian researchers tested the ecotoxicological effects of combining polyethylene microplastics with the insecticide imidacloprid on the freshwater crustacean Daphnia, finding combined exposures were more toxic than either pollutant alone. These results suggest that microplastics and pesticides together pose greater risks to aquatic organisms than studies of single pollutants indicate.

2023 Digital Library of Theses and Dissertations (Universidade de São Paulo)
Article Tier 2

Co-exposure of microplastics and 2-methoxy-1,4-naphthoquinone affects Daphnia magna depending on the developmental stage

Researchers examined the combined effects of microplastic exposure and the naturally occurring chemical 2-methoxy-1,4-naphthoquinone on Daphnia magna, investigating how plastic particles interact with an invasive species-linked chemical stressor in freshwater. Co-exposure produced different effects than either stressor alone, illustrating how microplastics complicate toxicity in multi-stressor freshwater environments.

2024 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Combined toxicity of perfluoroalkyl substances and microplastics on the sentinel species Daphnia magna: Implications for freshwater ecosystems

This study tested how PFAS chemicals (common industrial pollutants) and PET microplastics affect water fleas, both alone and together. The combination caused worse developmental and reproductive problems than either pollutant alone, and organisms with prior chemical exposure history responded differently, showing that microplastics can amplify the harm of other environmental contaminants in ways that are difficult to predict.

2024 Environmental Pollution 37 citations
Article Tier 2

Microplastics and plastic additives as contaminants of emerging concern: A multi-biomarker approach using Rhinella arenarum tadpoles

Researchers exposed toad tadpoles to polyethylene microplastics and the flame retardant TBBPA, both alone and in combination, for 30 days. They found that the mixture produced different toxic effects than either substance alone, affecting growth, enzyme activity, and cellular stress markers. The study highlights the importance of studying microplastics alongside common plastic additives, since their combined effects may differ from individual exposures.

2023 Environmental Advances 17 citations
Article Tier 2

Multi-omics characterisation of Daphnia magna exposed to PFAS and microplastics: transcriptome and gut microbiome datasets

Researchers generated a multi-omics dataset from Daphnia magna exposed to environmentally relevant concentrations of PFOS, PFOA, and PET microplastics, integrating gut microbiome 16S rRNA profiling and whole-organism transcriptomes to enable systems-level investigation of host-microbiome interactions under complex contaminant stress.

2025
Article Tier 2

Insights into the mechanisms of within-species variation in sensitivity to chemicals: A case study using daphnids exposed to CMIT/MIT biocide

Researchers compared how two strains of the water flea Daphnia pulex responded to a common industrial biocide and found that differences in sensitivity between strains of the same species were as large as differences between separate species, with epigenetic modifications (chemical tags on DNA) playing a key role in determining toxicity outcomes.

2023 Ecotoxicology and Environmental Safety 4 citations
Article Tier 2

Machine Learning Models for Identification and Prediction of Toxic Organic Compounds Using Daphnia magna Transcriptomic Profiles

Researchers used transcriptomic profiles from Daphnia magna to train machine learning models capable of identifying and predicting toxicity from 22 organic pollutants in aquatic environments. The models outperformed conventional toxicity tests by providing mechanistic insights into how specific contaminants affect gene expression pathways.

2022 Life 11 citations
Article Tier 2

Toxicological interactions induced by chronic exposure to gold nanoparticles and microplastics mixtures in Daphnia magna

This study examined the combined toxicological effects of gold nanoparticles and microplastics through chronic exposure, finding interactive effects that differed from either contaminant alone, emphasizing the importance of studying multiple stressors together.

2018 The Science of The Total Environment 154 citations
Article Tier 2

Complex Mixtures and Multiple Stressors: Evaluating Combined Chemical Exposures and Cumulative Toxicity

This review examined how complex chemical mixtures and multiple stressors interact to produce cumulative toxicity, highlighting that traditional single-chemical risk assessments underestimate real-world exposure risks where organisms face simultaneous pollutant combinations.

2023 Toxics 10 citations
Article Tier 2

Advances in aquatic toxicology for predicting effects of multiple pollutants on aquatic organisms

This review examines advances in aquatic toxicology for predicting how mixtures of contaminants — heavy metals, pesticides, pharmaceuticals, and microplastics — interact in aquatic organisms, highlighting computational modeling and mixture toxicity approaches as key tools for environmental risk assessment.

2025 International Journal of Aquatic Research and Environmental Studies
Article Tier 2

Assessment of intake and effect of microplastics and its combination with metals in experimental (Daphnia magna) and environmental conditions (freshwater fish)

Researchers assessed the intake and effects of microplastics and their combination with metals using Daphnia magna as an experimental model and freshwater fish under environmental conditions, investigating how microplastics adsorb and transport harmful metals in freshwater systems.

2025 Repository of Faculty of Chemical Engineering and Technology University of Zagreb
Article Tier 2

Multi-Biomarker Responses of Asian Clam Corbicula fluminea (Bivalvia, Corbiculidea) to Cadmium and Microplastics Pollutants

Researchers exposed Asian clams to cadmium, microplastics, and their mixtures, then measured a battery of biomarkers including oxidative stress, energy metabolism, and neurotoxicity indicators. They found that the combined exposure to cadmium and microplastics produced interactive effects that differed from exposure to either contaminant alone. The study demonstrates that microplastics can modify the toxicity of heavy metals in freshwater bivalves, highlighting the importance of studying pollutant mixtures rather than individual contaminants.

2021 Water 61 citations
Article Tier 2

Exploring the combined toxic effects of tri-n-butyl phosphate and polystyrene micro/nano-plastics on Daphnia magna under environmentally relevant concentrations

Researchers explored the combined toxic effects of the flame retardant tri-n-butyl phosphate and polystyrene micro- and nanoplastics on the water flea Daphnia magna at environmentally relevant concentrations. The study found that co-exposure to both pollutants altered gene expression related to stress responses, suggesting that the combined presence of these emerging contaminants may pose greater risks to aquatic organisms than either pollutant alone.

2025 Environmental Geochemistry and Health 1 citations