Papers

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Article Tier 2

Combined Enterohepatic Toxicity of Polystyrene Microplastics and Di(2-ethylhexyl) Phthalate in Mice: Gut Microbiota-Dependent Synergistic Effects

Researchers investigated the combined toxicity of polystyrene microplastics and the plasticizer DEHP in mice, focusing on gut-liver axis effects. They found that co-exposure worsened harmful outcomes compared to either pollutant alone, with gut microbiota playing a key mediating role in the synergistic toxicity. The study suggests that microplastics and their associated chemical additives may interact to amplify health risks through disruption of the gut-liver connection.

2026 Environmental Pollution
Article Tier 2

Combined exposure to polyvinyl chloride and polystyrene microplastics induces liver injury and perturbs gut microbial and serum metabolic homeostasis in mice

Mice exposed to a combination of PVC and polystyrene microplastics for 60 days developed liver damage, gut barrier breakdown, and disrupted gut bacteria. The co-exposure also raised cholesterol and triglyceride levels in both blood and liver, and altered hundreds of metabolites related to fat metabolism. Since people are typically exposed to multiple types of microplastics simultaneously, this study suggests the combined effects may be worse than exposure to a single type alone.

2023 Ecotoxicology and Environmental Safety 30 citations
Article Tier 2

Synergistic Toxicity of Combined Exposure to Acrylamide and Polystyrene Nanoplastics on the Gut–Liver Axis in Mice

Researchers exposed mice to a combination of acrylamide, a common food processing byproduct, and polystyrene nanoplastics through drinking water and found that the combined exposure caused more severe gut and liver damage than either substance alone. The co-exposure disrupted gut barrier integrity, altered gut bacteria composition, and caused widespread metabolic changes. The study suggests that the interaction between nanoplastics and other food contaminants may amplify health risks beyond what each poses individually.

2025 Biology 3 citations
Article Tier 2

Effects of microplastics and tetracycline on intestinal injury in mice

Researchers found that mice exposed to both microplastics and the antibiotic tetracycline suffered more intestinal damage than those exposed to either pollutant alone. The combined exposure caused distinct injuries across different segments of the intestine and disrupted gut bacteria composition. This is concerning because humans are commonly exposed to both microplastics and antibiotic residues through food and water.

2023 Chemosphere 39 citations
Article Tier 2

Microplastic-contaminated antibiotics as an emerging threat to mammalian liver: enhanced oxidative and inflammatory damages

Researchers used a mouse model to study what happens when microplastics contaminated with antibiotics are ingested together, simulating real-world food chain exposure. The study found that the combination caused enhanced oxidative stress and inflammatory damage in the liver compared to either pollutant alone. The findings suggest that microplastics carrying adsorbed antibiotics may pose a greater threat to liver health than microplastics or antibiotics individually.

2023 Biomaterials Science 32 citations
Article Tier 2

Polystyrene nanoplastics exacerbate aflatoxin B1-induced hepatic injuries by modulating the gut−liver axis

Mice exposed to both polystyrene nanoplastics and aflatoxin B1, a common food contaminant from mold, suffered worse liver damage than from either pollutant alone. The nanoplastics disrupted gut bacteria and weakened the intestinal barrier, allowing more toxins to reach the liver through the gut-liver axis. This study is concerning because it shows that microplastics can amplify the harmful effects of other food contaminants people are already exposed to.

2024 The Science of The Total Environment 13 citations
Article Tier 2

Effects of microplastics and tetracycline induced intestinal damage, intestinal microbiota dysbiosis, and antibiotic resistome: metagenomic analysis in young mice

Young mice exposed to both polystyrene microplastics and the antibiotic tetracycline suffered worse intestinal damage than those exposed to either pollutant alone. The combination severely disrupted the gut barrier, altered gut bacteria, and increased antibiotic resistance genes in the intestines. This is especially concerning for children, whose developing gut systems may be more vulnerable to the combined effects of microplastics and antibiotics commonly found in the environment.

2025 Environment International 9 citations
Article Tier 2

The interaction between polyethylene microplastics and ciprofloxacin on inducing hepatotoxicity in Carassius auratus via the gut-liver axis

Researchers co-exposed crucian carp to polyethylene microplastics and the antibiotic ciprofloxacin and assessed liver toxicity through the gut-liver axis. The combination caused greater hepatic injury than either contaminant alone—disrupting gut microbiota, increasing intestinal permeability, and amplifying liver inflammation—highlighting synergistic toxicity when fish are exposed to both antibiotic and plastic pollution.

2025 Environmental Pollution
Article Tier 2

Polystyrene microplastics exacerbated liver injury from cyclophosphamide in mice: Insight into gut microbiota

Researchers developed a mouse model to investigate whether chronic pre-exposure to polystyrene microplastics worsens liver injury caused by the drug cyclophosphamide. The study found that mice with 90 days of microplastic exposure showed significantly more severe liver damage when subsequently treated with cyclophosphamide, with changes linked to gut microbiota disruption. The findings suggest that chronic microplastic exposure may reduce the liver's resilience to additional chemical stressors.

2022 The Science of The Total Environment 73 citations
Article Tier 2

Combined effect of microplastic and triphenyltin: Insights from the gut-brain axis

Researchers investigated the individual and combined toxicity of microplastics and triphenyltin, an organotin compound, in common carp by examining effects along the gut-brain axis. The study found that co-exposure to microplastics and triphenyltin produced combined toxic effects on the gut microbiome and brain function, suggesting that microplastics may enhance the toxicity of other environmental pollutants through their ability to adsorb contaminants.

2023 Environmental Science and Ecotechnology 27 citations
Article Tier 2

Interactions of microplastics and organic compounds in aquatic environments: A case study of augmented joint toxicity

Researchers investigated how polystyrene microplastics interact with the antimicrobial compound triclosan in simulated environmental and cellular conditions. They found that surface-functionalized microplastics adsorbed significantly more triclosan and released it under cellular conditions, with the combination producing greater toxicity to human intestinal cells than either contaminant alone. The study suggests that microplastics can amplify the harmful effects of co-occurring organic pollutants.

2021 Chemosphere 77 citations
Article Tier 2

Oral exposure to polyethylene microplastics of adult male mice fed a normal or western-style diet: impact on gut and gut-liver axis homeostasis

Researchers exposed adult male mice to polyethylene microplastics on normal or Western diet for 90 days, examining synergistic effects between plastic and dietary stress on gut and liver health. Microplastic exposure disrupted gut barrier integrity, altered the microbiome, and affected liver homeostasis, with some effects differing between normal and Western diet groups.

2024 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Combined toxicity of polystyrene microplastics and perfluorobutane sulfonate on mouse liver: Impact on lipid metabolism and gut-liver axis disruption

This study examined what happens when mice are exposed to both polystyrene microplastics and PFBS (a type of "forever chemical") at the same time. The combination caused significantly worse liver damage than either pollutant alone, disrupting fat metabolism and triggering gut bacteria imbalances that further harmed the liver through the gut-liver connection. These findings are concerning because microplastics can absorb PFAS chemicals in the environment, meaning people may often be exposed to both together.

2025 Ecotoxicology and Environmental Safety 21 citations
Article Tier 2

New insight into intestinal toxicity accelerated by aged microplastics with triclosan: Inflammation regulation by gut microbiota-bile acid axis

Researchers exposed frogs to aged microplastics carrying triclosan, an antimicrobial chemical commonly found in personal care products, and found the combination was more toxic to the gut than either pollutant alone. The microplastics increased triclosan absorption in the gut by 89%, disrupting gut bacteria and bile acid metabolism, which led to intestinal inflammation. This study shows that microplastics can act as carriers that amplify the harmful effects of other chemicals already present in the environment.

2025 Journal of Hazardous Materials 9 citations
Article Tier 2

Gut Microbiota Participates in Polystyrene Microplastics-Induced Hepatic Injuries by Modulating the Gut–Liver Axis

This mouse study showed that polystyrene microplastics cause liver damage partly through disrupting gut bacteria, which then triggers harmful signals along the gut-liver connection. When researchers eliminated gut bacteria with antibiotics, liver damage from microplastics was reduced, confirming the gut microbiome plays a key role. Green tea extract (EGCG) helped protect the liver by restoring healthy gut bacteria, suggesting diet may help counteract some effects of microplastic exposure.

2023 ACS Nano 160 citations
Article Tier 2

Gut dysbiosis exacerbates inflammatory liver injury induced by environmentally relevant concentrations of nanoplastics via the gut-liver axis

This mouse study found that swallowing nanoplastics at levels found in the environment disrupted gut bacteria and damaged the intestinal barrier, allowing toxins to leak into the bloodstream and cause liver inflammation. When researchers transplanted gut bacteria from nanoplastic-exposed mice into healthy mice, those mice also developed liver damage. This demonstrates that nanoplastics may harm the liver indirectly by first disrupting the gut, a finding relevant to understanding how everyday plastic exposure could affect human health.

2024 Journal of Environmental Sciences 16 citations
Article Tier 2

Insights into the synergistic toxicity mechanisms caused by nano- and microplastics with triclosan using a dose-dependent functional genomics approach in Saccharomyces cerevisiae

Researchers used yeast functional genomics to investigate the combined toxicity of polystyrene nano- and microplastics with the antimicrobial compound triclosan. They found that the combined exposure produced synergistic toxic effects that were more harmful than either contaminant alone, disrupting cellular processes related to membrane integrity and protein function. The study provides molecular-level evidence that microplastics may amplify the toxicity of co-occurring chemical pollutants.

2024 Chemosphere 3 citations
Article Tier 2

Synergistic toxicity of nanoplastics and Helicobacter pylori on digestive system in mice

Researchers studied the combined toxic effects of nanoplastics and the stomach bacterium Helicobacter pylori on the digestive systems of mice. They found that co-exposure caused more severe damage to the stomach, colon, and liver than either stressor alone, including increased inflammation and disrupted gut barrier function. The study suggests that nanoplastic contamination may worsen the health effects of common gut infections.

2025 Ecotoxicology and Environmental Safety 3 citations
Article Tier 2

Polystyrene microplastics exposure increases the disruption of intestinal barrier integrity and gut microbiota homeostasis during obesity and aging

Researchers found that polystyrene microplastic exposure worsened intestinal barrier dysfunction in mice on high-fat diets, with the combination of obesity and microplastic exposure producing greater gut permeability and inflammation than either factor alone, suggesting compounding risks in metabolically vulnerable individuals.

2025 Journal of Environmental Sciences
Article Tier 2

Nanoplastics and triclosan co-exposure aggravates DSS-induced colitis in mice by interfering with Akkermansia muciniphila and tryptophan metabolism

Mouse colitis experiments showed that combined exposure to polystyrene nanoplastics and the antimicrobial triclosan worsened intestinal inflammation by depleting Akkermansia muciniphila and disrupting tryptophan metabolism, revealing synergistic gut-disrupting effects of these two common environmental pollutants.

2025 Journal of Environmental Sciences
Article Tier 2

Effects of microplastics (MPs) and tributyltin (TBT) alone and in combination on bile acids and gut microbiota crosstalk in mice

Researchers studied the combined effects of microplastics and tributyltin (TBT), an environmental pollutant, on bile acid metabolism and gut microbiota in mice. The study found that both individual and combined exposures induced liver inflammation, altered gut microbial composition, and disturbed bile acid profiles, suggesting that co-exposure to microplastics and chemical pollutants may have compounding effects on gut health.

2021 Ecotoxicology and Environmental Safety 66 citations
Article Tier 2

Gut microbiota related response of Oryzias melastigma to combined exposure of polystyrene microplastics and tetracycline

Researchers exposed estuarine fish to polystyrene microplastics and the antibiotic tetracycline, both alone and in combination, for four weeks. The combined exposure caused more severe disruption to gut bacteria and liver tissue than either pollutant alone, with microplastics appearing to worsen the effects of tetracycline. The study suggests that the co-occurrence of microplastics and antibiotics in coastal waters may pose greater ecological risks than either contaminant by itself.

2023 The Science of The Total Environment 18 citations
Article Tier 2

Oral exposure of polystyrene microplastics and doxycycline affects mice neurological function via gut microbiota disruption: The orchestrating role of fecal microbiota transplantation

Mice exposed to both polystyrene microplastics and the antibiotic doxycycline showed brain inflammation and declines in learning and memory, driven by disruptions to their gut bacteria. Fecal transplants from healthy mice reversed some of these brain effects, confirming the gut-brain connection plays a key role. This suggests that microplastics combined with common antibiotics could harm brain function through changes in the gut microbiome.

2024 Journal of Hazardous Materials 39 citations
Article Tier 2

Environmentally Relevant Concentrations of Microplastic Exposure Cause Cholestasis and Bile Acid Metabolism Dysregulation through a Gut-Liver Loop in Mice

Mice exposed to environmentally realistic levels of polystyrene microplastics for 30 days developed damaged intestinal barriers, liver injury, and disrupted bile acid metabolism. The study revealed a gut-liver feedback loop where microplastics alter gut bacteria, which changes bile acid production, which in turn causes further liver damage, suggesting a mechanism by which everyday microplastic exposure could harm digestive health.

2024 Environmental Science & Technology 69 citations