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Oral exposure to polyethylene microplastics of adult male mice fed a normal or western-style diet: impact on gut and gut-liver axis homeostasis

Zenodo (CERN European Organization for Nuclear Research) 2024 Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Muriel Mercier-Bonin, Muriel Mercier-Bonin Muriel Mercier-Bonin Muriel Mercier-Bonin, Catherine Beaufrand, Muriel Mercier-Bonin Yann Malaisé, Catherine Beaufrand, Catherine Beaufrand, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Valérie Bézirard, Yann Malaisé, Yann Malaisé, Catherine Beaufrand, Rémi Dages, Catherine Beaufrand, Catherine Beaufrand, Catherine Beaufrand, Catherine Beaufrand, Rémi Dages, Rémi Dages, Rémi Dages, Rémi Dages, Christel Cartier, Christel Cartier, Christel Cartier, Christel Cartier, Rémi Dages, Cécile Levasseur‐Garcia, Rémi Dages, Rémi Dages, Rémi Dages, Rémi Dages, Christel Cartier, Cécile Levasseur‐Garcia, Rémi Dages, Rémi Dages, Eric Houdeau, Eric Houdeau, Eric Houdeau, Eric Houdeau, Eric Houdeau, Valérie Bézirard, Valérie Bézirard, Bruno Lamas, Bruno Lamas, Valérie Bézirard, Bruno Lamas, Valérie Bézirard, Bruno Lamas, Hervé Robert, Hervé Robert, Bruno Lamas, Hervé Robert, Hervé Robert, Bruno Lamas, Hervé Robert, Hervé Robert, Muriel Mercier-Bonin Hervé Robert, Hervé Robert, Hervé Robert, Hervé Robert, Muriel Mercier-Bonin, Muriel Mercier-Bonin Muriel Mercier-Bonin, Muriel Mercier-Bonin

Summary

Researchers exposed adult male mice to polyethylene microplastics on normal or Western diet for 90 days, examining synergistic effects between plastic and dietary stress on gut and liver health. Microplastic exposure disrupted gut barrier integrity, altered the microbiome, and affected liver homeostasis, with some effects differing between normal and Western diet groups.

Polymers

Body Systems

Digestive Hepatic Immune

Models

Mouse

Study Type In vivo

Plastics in the environment are fragmented into small particles known as microplastics (MPs). In humans, MPs have been detected in stools, attesting to their transit along the gastrointestinal tract. Few studies have assessed their potential effects on the digestive tract in at-risk populations. Based on this background, we aimed at studying the consequences of oral exposure to polyethylene (PE) MPs under nutritional stress. Adult male mice were daily exposed to PE MPs on a Western (WD) vs. normal (ND) diet for 90 days, to reveal any exacerbating/synergistic effects between MP and nutritional stresses. The following assays were performed in mice exposed to ND or WD with or without MPs: follow-up of body weight, food and water intake; oral glucose tolerance test; measurement of in vivo intestinal permeability (using FITC-labeled dextran), colonic lipocalin-2 level, and distal colon mucus thickness (Alcian Blue staining); fecal microbiota composition (16S rDNA gene metabarcoding); gene expression in small intestine and colon (inflammation, tight junctions, antimicrobial peptides, mucus biosynthesis and secretion), gene expression and histology in liver (size and size distribution of lipid droplets using Red Oil staining). The effects of WD on gut homeostasis impairment were demonstrated, including defects in epithelium and mucus barriers as well as shifts in fecal bacterial communities. Liver homeostasis was also disrupted. Concerning the impact of MP exposure with ND or WD, results on the gut barrier function and gut-liver axis revealed signatures common or specific to each exposure condition. To support this toxicological assessment, and as a biophysics-based innovation in the field, near-infrared spectroscopy (NIRS), coupled to chemometrics, was used to monitor, in all exposed groups, changes in the intestine (contents, tissues) and the liver. This work enables a better assessment of the hazards of oral exposure to MPs in an at-risk WD-fed population (altered intestinal barrier, dysbiotic gut microbiota). Also see: https://micro2024.sciencesconf.org/558619/document

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