Papers

20 results
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Article Tier 2

Transcriptomic and metabolic responses of earthworms to contaminated soil with polypropylene and polyethylene microplastics at environmentally relevant concentrations

Researchers studied how environmentally realistic concentrations of polypropylene and polyethylene microplastics affect earthworms at the molecular level. They found that both plastic types triggered oxidative stress, damaged digestive and immune systems, disrupted lipid metabolism, and altered the earthworms' ability to regulate water balance. The study suggests that even at concentrations commonly found in the environment, microplastic-contaminated soil poses measurable health risks to earthworms.

2021 Journal of Hazardous Materials 126 citations
Article Tier 2

The effects of polyethylene microplastics on the growth, reproduction, metabolic enzymes, and metabolomics of earthworms Eisenia fetida

This study exposed earthworms to polyethylene microplastics in soil for 60 days and found that even when the worms appeared physically healthy, their internal enzyme systems and metabolism were significantly disrupted. The damage included signs of neurotoxicity, oxidative stress, and reduced ability to break down harmful substances. Since earthworms are essential for soil health and crop growth, this disruption could indirectly affect the quality of food humans eat.

2023 Ecotoxicology and Environmental Safety 43 citations
Article Tier 2

Oxidative stress, energy metabolism and molecular responses of earthworms (Eisenia fetida) exposed to low-density polyethylene microplastics

Researchers exposed earthworms to various concentrations of low-density polyethylene microplastics for 28 days and measured oxidative stress, energy metabolism, and molecular responses. The study found dose-dependent increases in oxidative damage markers and alterations in energy reserves, suggesting that microplastic contamination in soils can trigger measurable physiological stress in soil invertebrates.

2018 Environmental Science and Pollution Research 199 citations
Article Tier 2

Effect of polyethylene microplastics on tebuconazole bioaccumulation, oxidative stress, and intestinal bacterial community in earthworms

Researchers exposed earthworms to polyethylene microplastics of different sizes alongside a common fungicide and found that smaller microplastics caused the most severe oxidative stress and DNA damage. The microplastics also changed how much fungicide accumulated in the earthworms and disrupted their gut bacteria. This matters because earthworms are essential for soil health, and these effects could ripple through agricultural ecosystems that produce our food.

2024 Journal of Hazardous Materials 12 citations
Article Tier 2

Nanoplastics induce molecular toxicity in earthworm: Integrated multi-omics, morphological, and intestinal microorganism analyses

Researchers used multi-omics analysis to study how even low concentrations of nanoplastics affect earthworms, important indicators of soil health. They found that nanoplastics accumulated in the earthworms' intestines, damaging their digestive and immune systems and disrupting gut microorganism communities. The study demonstrates that nanoplastics can cause molecular-level harm to soil organisms at concentrations that might be considered environmentally realistic.

2022 Journal of Hazardous Materials 86 citations
Article Tier 2

Earthworms on a microplastics diet

Researchers found that environmentally relevant concentrations of polyethylene microplastics added to plant litter on soil surfaces led to reduced growth and elevated mortality in the earthworm Lumbricus terrestris, and that earthworms may themselves transport ingested microplastics deeper into soils.

2016 Science 4 citations
Article Tier 2

Microplastic pollution destabilized the osmoregulatory metabolism but did not affect intestinal microbial biodiversity of earthworms in soil

This study investigated how microplastic pollution affects the ability of aquatic organisms to regulate their internal salt and water balance. Researchers found that microplastic exposure disrupted key metabolic pathways involved in osmoregulation, though it did not significantly affect overall survival rates in the short term. The findings suggest that even when animals appear healthy, microplastics may be causing hidden physiological stress.

2023 Environmental Pollution 46 citations
Article Tier 2

Understanding the harmful effects of polyethylene microplastics on Eisenia fetida: A toxicological evaluation

Earthworms (Eisenia fetida) exposed to increasing concentrations of polyethylene microplastics in soil showed lower body weight, reduced reproductive output, and disrupted antioxidant defenses — with oxidative stress markers climbing nearly 1.3-fold at the highest dose. These findings confirm that microplastic pollution degrades soil ecosystem health at concentrations that could plausibly occur in contaminated agricultural land.

2023 Journal of Applied and Natural Science 1 citations
Article Tier 2

Histopathological and molecular effects of microplastics in Eisenia andrei Bouché

Researchers exposed earthworms to polyethylene microplastics in soil and examined the effects on their tissues and gene expression. They found that microplastic exposure caused visible damage to the earthworms' gut lining and skin, and altered the activity of genes involved in stress response and immune function. The study provides some of the first evidence that microplastics can harm soil organisms at both the tissue and molecular level.

2016 Environmental Pollution 576 citations
Article Tier 2

Effects of polyethylene microplastics stress on soil physicochemical properties mediated by earthworm Eisenia fetida

Researchers exposed earthworms to polyethylene microplastics of two sizes and found that smaller particles (13 micrometers) were more toxic than larger ones (130 micrometers), reducing survival and growth more severely. The microplastics caused oxidative stress in the worms and altered key soil properties including pH and organic carbon content. Since earthworms play a vital role in maintaining healthy soil for agriculture, this damage could affect soil quality and ultimately the food grown in microplastic-contaminated farmland.

2024 Environmental Science and Pollution Research 14 citations
Article Tier 2

The effects of high-density polyethylene and polypropylene microplastics on the soil and earthworm Metaphire guillelmi gut microbiota

Researchers exposed earthworms to soil amended with high-density polyethylene and polypropylene microplastics for 28 days and examined changes in both the earthworm gut and soil microbial communities. They found that both types of microplastics significantly altered the composition and diversity of gut bacteria in the earthworms. The study suggests that microplastic contamination in soil can disrupt the gut microbiota of soil organisms, with potential consequences for soil ecosystem health.

2020 Chemosphere 142 citations
Article Tier 2

Adverse effects of microplastics on earthworms: A critical review

This critical review of 65 publications summarized the adverse effects of microplastics on earthworms, finding impacts on growth, behavior, oxidative stress, gene expression, and gut microbiota, with particle size, concentration, and co-occurring pollutants influencing toxicity outcomes.

2022 The Science of The Total Environment 111 citations
Article Tier 2

Accumulation of microplastics and Tcep pollutants in agricultural soil: Exploring the links between metabolites and gut microbiota in earthworm homeostasis

Researchers investigated the co-occurrence of polyethylene microplastics and the flame retardant TCEP in agricultural soils and their combined effects on earthworm health. The study found that co-exposure disrupted earthworm gut microbiota and metabolic homeostasis, suggesting that the interaction between microplastics and chemical additives in agricultural soil may pose greater ecological risks than either contaminant alone.

2022 Environment International 59 citations
Article Tier 2

Responses of earthworms exposed to low-density polyethylene microplastic fragments

Researchers exposed earthworms to low-density polyethylene microplastic fragments at various concentrations and studied the effects on their survival, growth, and reproduction. The microplastics affected earthworm behavior and caused measurable changes depending on concentration and exposure time. Since earthworms are critical for soil health and nutrient cycling, their sensitivity to microplastics raises concerns about how plastic pollution may degrade agricultural soils.

2023 Chemosphere 31 citations
Article Tier 2

Reproduction, metabolic enzyme activity, and metabolomics in earthworms Eisenia fetida exposed to different polymer microplastics

Researchers exposed earthworms to microplastics from three different polymer types, including both conventional and biodegradable plastics, at environmentally relevant concentrations. They found that polypropylene microplastics had the most pronounced effects on reproduction and metabolic enzyme activity, while biodegradable plastics also disrupted earthworm metabolism. The study demonstrates that different plastic polymers pose varying levels of risk to soil-dwelling organisms.

2025 Ecotoxicology and Environmental Safety 2 citations
Article Tier 2

Microplastic digestion generates fragmented nanoplastics in soils and damages earthworm spermatogenesis and coelomocyte viability

Researchers discovered that earthworms can fragment polyethylene microplastics into even smaller nanoplastics through their digestive process in soil. The study also found that microplastic exposure damaged earthworm reproductive cells and immune cells, suggesting that the biological breakdown of microplastics in soil creates smaller particles that may be even more concerning for ecosystem health.

2020 Journal of Hazardous Materials 344 citations
Article Tier 2

Negligible effects of microplastics on animal fitness and HOC bioaccumulation in earthworm Eisenia fetida in soil

Researchers exposed earthworms to polyethylene and polystyrene microplastics at concentrations up to 20 percent of soil dry weight and measured oxidative stress biomarkers. While the highest concentration caused some biochemical changes, no significant effects were observed at 10 percent or below, which covers most realistic environmental scenarios. The study also found that microplastics reduced the bioaccumulation of PAHs and PCBs in earthworm tissues, suggesting that the particles may actually limit the uptake of certain organic pollutants in soil organisms.

2019 Environmental Pollution 338 citations
Article Tier 2

Short-Term Effect of Poly Lactic Acid Microplastics Uptake by Earthworms, Eudrilus eugeniae.

Researchers tested whether earthworms (Eudrilus eugeniae) would ingest polylactic acid (PLA) biodegradable microplastics when mixed with organic matter, finding they did but with reduced weight gain and reproductive output at higher concentrations. This suggests even biodegradable microplastics can harm soil organisms, which are essential for nutrient cycling and soil health.

2021
Article Tier 2

Microplastic Moves Pollutants and Additives to Worms, Reducing Functions Linked to Health and Biodiversity

Researchers exposed lugworms to sediment containing 5% microplastic pre-loaded with pollutants and plastic additives, confirming that ingested microplastic transfers chemicals into gut tissue — with nonylphenol uptake from PVC reducing immune cell function by over 60% and triclosan from PVC causing sediment-engineering impairment and mortality exceeding 55%.

2013 Current Biology 1120 citations
Article Tier 2

Earthworms Exposed to Polyethylene and Biodegradable Microplastics in Soil: Microplastic Characterization and Microbial Community Analysis

Researchers exposed earthworms to biodegradable and conventional polyethylene microplastics in natural soil and found that worms ingested both types. The biodegradable plastic showed signs of partial breakdown in the earthworm gut, while conventional polyethylene remained unchanged. Although microplastics did not significantly alter the soil or gut microbiome in this study, the results confirm that earthworms transport microplastics through soil ecosystems.

2023 ACS Agricultural Science & Technology 42 citations