Papers

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Article Tier 2

Polystyrene Microplastics Affect the Reproductive Performance of Male Mice and Lipid Homeostasis in Their Offspring

Researchers found that long-term exposure to environmentally relevant doses of polystyrene microplastics over 21 weeks significantly impaired reproductive function in male mice, including decreased testicle weight and sperm quality. The study also revealed transgenerational effects, with offspring showing disrupted lipid homeostasis.

2022 Environmental Science & Technology Letters 53 citations
Article Tier 2

Polystyrene microplastics induced male reproductive toxicity and transgenerational effects in freshwater prawn

Researchers found that polystyrene microplastics caused significant reproductive harm in male freshwater prawns, including oxidative stress in testis tissue, hormonal imbalances, and reduced sperm quality. Notably, the offspring of exposed prawns also showed reduced survival and weakened immunity even when they were not directly exposed to microplastics. The study reveals that microplastic exposure can produce transgenerational effects in aquatic organisms, passing harm from parents to offspring.

2022 The Science of The Total Environment 85 citations
Article Tier 2

Impact of polystyrene microplastic exposure at low doses on male fertility: an experimental study in rats

Researchers exposed adult male rats to varying doses of polystyrene microplastics and found dose-dependent declines in semen quality along with disrupted reproductive hormone levels. Higher doses caused increased oxidative stress, mitochondrial damage, and inflammatory responses in testicular tissue. The study suggests that even relatively low doses of microplastic exposure may have adverse effects on male reproductive health in animal models.

2026 Scientific Reports
Article Tier 2

Polystyrene Microplastics Disrupt Spermatogenesis through Oxidative Stress in Rat Testicular Tissue

Male Wistar rats orally administered polystyrene microplastics showed excessive oxidative stress in testicular tissue across all exposure groups, with spermatogenesis impairment and reduced fertility correlating with dose, demonstrating reproductive toxicity in a mammalian model.

2025 Journal of Pharmaceutical Research International
Article Tier 2

Dose-Dependent Effect of Polystyrene Microplastics on the Testicular Tissues of the Male Sprague Dawley Rats

Male rats exposed to increasing doses of polystyrene microplastics showed dose-dependent testicular damage including disrupted spermatogenesis and altered hormone levels, suggesting potential reproductive toxicity from microplastic accumulation.

2021 Dose-Response 98 citations
Article Tier 2

Direct and intergenerational effects in reproductive parameters of adult male Wistar rats and their offspring after subchronic exposure to polystyrene nanoplastics

Researchers exposed adult male Wistar rats to 500 nm polystyrene nanoplastics and assessed effects on reproductive parameters and intergenerational outcomes in offspring. PS-NP exposure reduced sperm quality and testosterone levels in exposed males, and some reproductive effects were observed in the next generation, suggesting potential intergenerational reproductive toxicity.

2025 Reproductive Toxicology
Article Tier 2

Intergenerational and transgenerational reproductive toxicity of polystyrene microplastics in female mice

Female mice were exposed to polystyrene microplastics during lactation and researchers tracked reproductive outcomes in both exposed mothers and their offspring through multiple generations, finding that even at doses comparable to human infant bottle-feeding exposure, microplastics induced ovarian damage and reduced fertility that persisted across generations.

2025 Global Reproductive Health
Article Tier 2

Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice

Researchers exposed pregnant mice and their offspring to polystyrene microplastics from gestation through early life and found significant disruption to testicular development and male reproductive function. The exposed males showed reduced sperm quality, lower testosterone levels, and structural damage to testicular tissue. The study suggests that early-life microplastic exposure may have lasting effects on male fertility.

2022 Journal of Hazardous Materials 116 citations
Article Tier 2

Chronic toxic effects of polystyrene microplastics on reproductive parameters of male rats

Researchers studied the chronic toxic effects of polystyrene microplastics on the reproductive system of male rats over 90 days. The study found significant reductions in sperm volume, motility, epididymal count, and serum testosterone levels, along with disrupted testicular architecture and decreased antioxidant capacity. The findings suggest that chronic microplastic exposure may adversely affect male reproductive parameters in mammals.

2022 Environmental Analysis Health and Toxicology 56 citations
Article Tier 2

The emerging risk of exposure to nano(micro)plastics on endocrine disturbance and reproductive toxicity: From a hypothetical scenario to a global public health challenge

Researchers administered polystyrene nanoplastics orally to male rats for five weeks and found significant reductions in testosterone, LH, and FSH levels, sperm DNA damage, altered testicular gene expression, and dose-dependent histological lesions, indicating that nanoplastic exposure disrupts the hormonal axis governing male reproductive function.

2020 Environmental Pollution 297 citations
Article Tier 2

Oral exposure to polystyrene nanoplastics altered the hypothalamic–pituitary–testicular axis role in hormonal regulation, inducing reproductive toxicity in albino rats

This study found that oral exposure to polystyrene nanoplastics disrupted the hormone signaling pathway between the brain and testes in male rats, leading to reproductive damage. The nanoplastics interfered with the hormones that regulate sperm production and testicular function. These findings add to growing evidence that nanoplastic exposure through food and water could be a contributing factor to declining male fertility.

2024 Birth Defects Research 16 citations
Article Tier 2

Reproductive Toxicity of Chronic Exposure To Polystyrene Microplastics And The Molecular Mechanism of Decrease In Testosterone Levels In Male Mice

Chronic exposure to polystyrene microplastics lowered testosterone levels in male mice and disrupted reproductive organ function. The study identified molecular pathways through which microplastics interfere with male hormone production, with implications for reproductive health in humans exposed through diet.

2021 Research Square (Research Square) 1 citations
Article Tier 2

Maternal exposure to polystyrene nanoplastics during gestation and lactation induces hepatic and testicular toxicity in male mouse offspring

Researchers exposed pregnant and nursing mice to polystyrene nanoplastics and studied the effects on their male offspring. The offspring showed reduced body weight, liver damage with inflammation and disrupted sugar metabolism, and testicular harm including decreased sperm counts. The findings suggest that nanoplastic exposure during pregnancy and breastfeeding can cause significant organ damage in the next generation.

2022 Food and Chemical Toxicology 168 citations
Article Tier 2

Effects of polystyrene microparticles exposures on spermatogenic cell differentiation and reproductive endpoints in male mice

Researchers found that very small polystyrene microplastics (0.1 micrometers) accumulated in mouse testicular tissue and sperm-producing cells, leading to reduced sperm quality and impaired reproductive function. The particles triggered oxidative stress and disrupted the normal process of sperm cell development. This study adds to growing evidence that microplastic exposure could contribute to male fertility problems in humans, particularly from the smallest particles that can penetrate reproductive tissues.

2025 Environmental Pollution 8 citations
Article Tier 2

Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice

Researchers exposed mice to polystyrene microplastics and examined the effects on male reproductive function. They found that microplastic exposure significantly reduced viable sperm count, increased sperm abnormalities, and caused structural damage to testicular tissue, suggesting that microplastics may pose risks to male fertility.

2020 Journal of Hazardous Materials 437 citations
Article Tier 2

Polystyrene microplastics induced male reproductive toxicity in mice

Researchers exposed male mice to polystyrene microplastics of different sizes and found that the particles accumulated in testicular tissue and entered reproductive cells. After 28 days of exposure, sperm quality and testosterone levels declined, and tissue examination revealed disorganized sperm-producing cells and inflammation. The study suggests that microplastic exposure may pose risks to male reproductive health in mammals.

2020 Journal of Hazardous Materials 607 citations
Article Tier 2

Impact of Polystyrene Microplastics on Human Sperm Functionality: An In Vitro Study of Cytotoxicity, Genotoxicity and Fertility-Related Genes Expression

Researchers exposed human sperm samples to polystyrene microplastics in the lab and observed decreased sperm vitality and motility in a time-dependent manner. The microplastics also caused DNA damage, increased harmful reactive oxygen species, and reduced the expression of genes essential for fertilization. The study suggests that microplastic exposure could impair male fertility through oxidative stress and interference with key reproductive functions.

2025 Toxics 2 citations
Article Tier 2

Polystyrene microplastics cause reproductive toxicity in male mice

Male mice exposed to polystyrene microplastics for six weeks showed significant reproductive damage, including reduced sperm count and motility, lower testosterone levels, and visible tissue damage in the testes. The microplastics caused oxidative stress and triggered cell death pathways in the reproductive tissue. These findings add to growing evidence that microplastic exposure could contribute to declining male fertility.

2024 Food and Chemical Toxicology 12 citations
Article Tier 2

Adolescent exposure to polystyrene nanoplastics induces male reproductive damage via the microbiome-gut-testis axis

Researchers exposed adolescent rats to polystyrene nanoplastics for five weeks and observed dose-dependent damage to testicular tissue, disrupted spermatogenesis, and compromised blood-testis barrier integrity. The study revealed a novel microbiome-gut-testis axis mechanism, where nanoplastics altered gut bacteria composition, which in turn contributed to reproductive toxicity in developing males.

2026 Journal of Nanobiotechnology
Article Tier 2

MicroRNA and Gut Microbiota Alter Intergenerational Effects of Paternal Exposure to Polyethylene Nanoplastics

In a mouse study, when fathers were exposed to polyethylene nanoplastics, their male offspring suffered reproductive damage including lower sperm counts and testicular injury — even though the offspring were never directly exposed. The effects were passed down through changes in small RNA molecules and gut bacteria, suggesting that nanoplastic exposure could affect fertility across generations.

2024 ACS Nano 19 citations
Article Tier 2

Impact of Ps-mps on the Functioning of Epididymis and Seminal Vesicle in Wistar Albino Rats

Researchers administered polystyrene microplastics to male Wistar rats at two dose levels and examined histological and functional changes in the epididymis and seminal vesicle. Microplastic exposure caused structural damage to both organs and disrupted secretory function, indicating that reproductive accessory glands are vulnerable to microplastic toxicity.

2025 International Journal of Current Research and Applied Studies
Article Tier 2

Maternal Exposure to Polystyrene Nanoplastics Disrupts Spermatogenesis in Mouse Offspring by Inducing Prdm14 Overexpression in Undifferentiated Spermatogonia

When pregnant mice were exposed to nanoplastics (extremely small plastic particles), their male offspring had lower testosterone, fewer sperm, and damaged sperm-producing cells. The nanoplastics caused inflammation in the cells responsible for sperm production, disrupting a key gene (Prdm14) that controls sperm development. This suggests that a mother's plastic exposure during pregnancy could affect her sons' fertility later in life.

2025 ACS Nano 16 citations
Article Tier 2

Low-dose polystyrene microplastics exposure impairs fertility in male mice with high-fat diet-induced obesity by affecting prostate function

Male mice exposed to low doses of microplastics had lower sperm quality and fewer offspring, and these effects were significantly worse when combined with a high-fat diet. The combination triggered inflammation and cell death in the prostate gland, reducing key nutrients in seminal fluid needed for sperm health. This suggests that microplastic exposure may be an overlooked factor in declining male fertility, especially for those with metabolic conditions like obesity.

2024 Environmental Pollution 20 citations
Article Tier 2

Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice

This study found that male mice given polystyrene nanoplastics by mouth showed significant harm to sperm function and early embryo development, with changes in gene expression that could affect offspring. The findings raise concerns that nanoplastic exposure could impair male fertility and potentially pass harmful effects to the next generation.

2025 Frontiers in Cell and Developmental Biology 10 citations