Papers

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Article Tier 2

Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability, pigment content, oxidative stress and ultrastructural changes

Researchers examined the toxic effects of different-sized polystyrene micro- and nanoplastics on the microalga Chlorella vulgaris in long-term exposure tests. The study found that smaller particles (20 and 50 nm) caused greater reductions in cell viability and chlorophyll concentration than larger ones, with surface functionalization also influencing toxicity and ultrastructural damage.

2020 Marine Pollution Bulletin 209 citations
Article Tier 2

Toxicity Effects of Polystyrene Nanoplastics with Different Sizes on Freshwater Microalgae Chlorella vulgaris

Researchers tested how two sizes of polystyrene nanoplastics (50 nm and 70 nm) affected the common freshwater microalgae Chlorella vulgaris. Both sizes reduced algae growth, chlorophyll content, and photosynthetic activity in a dose-dependent manner, with the smaller particles causing more damage. Since microalgae form the base of aquatic food chains, their sensitivity to nanoplastics could have cascading effects on entire freshwater ecosystems.

2023 Molecules 31 citations
Article Tier 2

Impacts of Microplastics on Photosynthetic Efficiency and Pigment Composition in Chlorella pyrenoidosa

Researchers evaluated how polyethylene and polystyrene microplastics at different concentrations affect photosynthesis and pigment composition in the microalga Chlorella pyrenoidosa over four days. They found that microplastic exposure impaired photosynthetic efficiency and altered chlorophyll and carotenoid levels. The study highlights the potential for microplastic pollution to disrupt primary producers at the base of aquatic food webs.

2024 European Journal of Theoretical and Applied Sciences 2 citations
Article Tier 2

Effects of different concentrations and particle sizes of microplastics on the full life history of freshwater Chlorella

Researchers investigated how polystyrene microplastics of different concentrations and particle sizes affect the complete life cycle of freshwater Chlorella algae. The study found that microplastics can inhibit algal growth by up to 68%, while also altering chlorophyll content and photosynthetic activity, indicating that microplastic pollution may pose significant risks to the base of aquatic food webs.

2024 Environmental Pollution 12 citations
Article Tier 2

Differential effect of nano vs. micro-sized plastics on live Chlorella sp. algae in water environment

Researchers exposed live Chlorella sp. algae to polystyrene particles ranging from 20 nm to 2000 nm and used confocal microscopy and fluorescence lifetime imaging to characterize interactions. Nanoplastics of 20–500 nm formed corona-like structures around algae cells and reduced chlorophyll fluorescence intensity and lifetime, indicating impaired photosynthesis, while larger 1000–2000 nm particles had minimal effects.

2025 Microplastics and Nanoplastics 2 citations
Article Tier 2

Toxic effects of polystyrene nanoplastics on microalgae Chlorella vulgaris: Changes in biomass, photosynthetic pigments and morphology

This study tested how polystyrene nanoplastics of three different sizes affect green algae and found a clear pattern: smaller particles were more toxic than larger ones. The smallest nanoplastics (90 nm) caused the greatest reductions in algal growth and photosynthetic pigments, along with visible changes in cell shape and increased clumping. The findings suggest that as plastics break down into ever-smaller particles in the environment, their potential for biological harm may increase.

2021 Chemosphere 112 citations
Article Tier 2

Concentration dependent toxicity of microplastics to marine microalgae

Researchers exposed the marine microalga Chlorella sp. to polystyrene microplastics at concentrations of 10 and 50 mg/L, finding that even low concentrations inhibited growth and disrupted photosynthesis, while higher concentrations caused more pronounced oxidative stress.

2025 E3S Web of Conferences
Article Tier 2

Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij

Researchers tested how polyethylene and polystyrene microplastics affect the freshwater algae Chlorella vulgaris and found that smaller particles and higher concentrations caused more harm. The microplastics reduced algal growth, photosynthetic efficiency, and disrupted nutrient composition over the 11-day experiment. Since algae form the base of aquatic food chains, this damage could ripple upward through ecosystems that ultimately connect to human food sources.

2023 Aquatic Toxicology 30 citations
Article Tier 2

Interactive Effects of Nano-Polystyrene and Light Spectra on Growth and Phytohormone (Auxin and Gibberellin) Production in Chlorella vulgaris

Researchers investigated how nanopolystyrene particles interact with different light conditions to affect the growth and hormone production of the green algae Chlorella vulgaris. Blue light enhanced algae growth and auxin production even in the presence of nanoplastics, while red light promoted gibberellin synthesis. The findings suggest that nanoplastic pollution could disrupt phytohormone balance in aquatic microalgae, with effects varying depending on environmental light conditions.

2025 Egyptian Journal of Aquatic Biology and Fisheries 1 citations
Article Tier 2

The impact of polystyrene nanoplastics (PSNPs) on physiological and biochemical parameters of the microalgae Spirulina platensis

Researchers exposed the microalgae Spirulina platensis to polystyrene nanoplastics at three concentrations over 20 days and found dose-dependent reductions in growth rate, dry weight, and photosynthetic pigments alongside increased oxidative stress markers, indicating nanoplastics impair algal physiology even at relatively low exposure levels.

2024 Journal of Hazardous Materials 13 citations
Article Tier 2

Effect of microplastics and microplastic-metal combinations on growth and chlorophyll a concentration of Chlorella vulgaris

Researchers tested the effects of polystyrene microplastics alone and in combination with metals (copper, zinc, manganese) on the freshwater microalga Chlorella vulgaris. The study found that low microplastic concentrations had no significant impact, but higher concentrations reduced algal growth and chlorophyll content, with metal-microplastic combinations producing more pronounced effects.

2020 The Science of The Total Environment 231 citations
Article Tier 2

Combined toxic effects of polystyrene nanoplastics and lead on Chlorella vulgaris growth, membrane lipid peroxidation, antioxidant capacity, and morphological alterations

Researchers found that amino-functionalized polystyrene nanoplastics and lead act synergistically to inhibit the growth of the microalga Chlorella vulgaris, with combined exposure producing greater reductions in chlorophyll, biomass, and cell size than either pollutant alone.

2024 Environmental Science and Pollution Research 9 citations
Article Tier 2

Phytoplankton response to polystyrene microplastics: Perspective from an entire growth period

Researchers tracked the effects of polystyrene microplastics on the green alga Chlorella pyrenoidosa across its entire growth cycle and found dose-dependent harm during early growth phases. The microplastics reduced photosynthetic activity and inhibited growth by up to 38%, though the algae showed some ability to recover in later growth stages. The study suggests that microplastics can meaningfully disrupt the growth of freshwater phytoplankton, which form the foundation of aquatic food webs.

2018 Chemosphere 669 citations
Article Tier 2

Dose-dependent effects of polystyrene nanoplastics on growth, photosynthesis, and astaxanthin synthesis in Haematococcus pluvialis

Researchers exposed the microalga Haematococcus pluvialis to polystyrene nanoplastics at various concentrations and found that higher doses significantly inhibited growth and photosynthesis. Interestingly, the stressed algae produced more astaxanthin, a natural antioxidant pigment, as a defense response. The study shows that nanoplastic pollution can disrupt algal growth while triggering biochemical stress responses in aquatic organisms.

2024 Environmental Pollution 8 citations
Article Tier 2

Nano-sized polystyrene plastics toxicity to microalgae Chlorella vulgaris: Toxicity mitigation using humic acid

Researchers exposed the green microalga Chlorella vulgaris to amino-functionalized polystyrene nanoplastics and found significant toxicity to biomass and chlorophyll, but adding humic acid — a natural organic matter component — substantially reduced toxicity by coating the nanoplastics' positively charged surfaces and preventing their electrostatic attraction to algal cell walls.

2022 Aquatic Toxicology 49 citations
Article Tier 2

Long-Term Effects of Nanoplastics on Growth, Photosynthesis, and Oxidative Stress in Asterococcus superbus

A 30-day nanoplastic exposure experiment on the freshwater alga Asterococcus superbus showed that long-term exposure caused progressive declines in growth rate, photosynthetic efficiency, and antioxidant capacity that were not evident in shorter acute toxicity studies.

2025 Journal of Environmental Engineering
Article Tier 2

Differential effect of nano vs. micro-sized plastics on live Chlorella sp. algae in water environment.

Researchers investigated how polystyrene microplastic and nanoplastic particles of different sizes (20 nm to 2000 nm) interact with Chlorella sp. algae using confocal microscopy and fluorescence lifetime imaging, finding that smaller particles (20-500 nm) formed corona-like structures around algae and disrupted chlorophyll photosynthesis, while larger particles (1000-2000 nm) acted as nucleation sites for algal clustering without affecting chlorophyll fluorescence.

2024
Article Tier 2

Microplastics and Heavy Metals Removal from Fresh Water and Wastewater Systems Using a Membrane

Researchers tested how polystyrene microplastics affect the growth, photosynthesis, and oxidative stress responses of freshwater microalgae Chlorella vulgaris. Smaller particles caused greater inhibition of growth and chlorophyll synthesis than larger ones.

2022 Separations 15 citations
Article Tier 2

Polystyrene Microplastics Induce Photosynthetic Impairment in Navicula sp. at Physiological and Transcriptomic Levels

Researchers exposed freshwater diatom algae to polystyrene microplastics and found significant damage to their photosynthetic capacity within 24 to 48 hours. The microplastics reduced chlorophyll content, damaged cell membranes, and triggered oxidative stress responses, with gene analysis revealing disruption of key pathways related to photosynthesis and carbon fixation. The findings suggest that microplastic pollution in freshwater environments could impair the ability of algae to produce oxygen and support aquatic food webs.

2024 International Journal of Molecular Sciences 9 citations
Article Tier 2

Polystyrene nanoplastics cause growth inhibition, morphological damage and physiological disturbance in the marine microalga Platymonas helgolandica

Researchers exposed marine green microalgae to polystyrene nanoplastics and found significant growth inhibition, increased membrane permeability, disrupted photosynthesis, and visible morphological damage — including surface fragmentation and cellular rupture — at concentrations as low as 200 µg/L.

2020 Marine Pollution Bulletin 128 citations
Article Tier 2

Effects of Polystyrene Microparticles on Growth and Physiological Metabolism of Microalgae Scendesmus obliquus

Researchers examined the toxic effects of polystyrene microparticles on the microalga Scenedesmus obliquus, finding that exposure inhibited growth and disrupted photosynthesis and antioxidant defense systems in a concentration-dependent manner.

2023 Sustainability 12 citations
Article Tier 2

The influence of microplastics on the toxic effects and biodegradation of bisphenol A in the microalgae Chlorella pyrenoidosa

Researchers found that polystyrene microplastics inhibited the biodegradation of bisphenol A (BPA) by the microalga Chlorella vulgaris, with combined exposure showing greater toxicity than either contaminant alone due to BPA adsorption onto microplastic surfaces.

2022 Research Square (Research Square) 4 citations
Article Tier 2

Recent progress on the toxic effects of microplastics on Chlorella sp. in aquatic environments

This review summarizes research on how microplastics affect Chlorella, a type of green algae that forms the base of aquatic food chains. Microplastics can slow algae growth, cause oxidative stress, and disrupt photosynthesis, which matters for human health because damage to these foundational organisms can ripple up through the food web and affect the quality of water and seafood.

2024 Heliyon 39 citations
Article Tier 2

Effects of polystyrene nanoplastics on the physiological and biochemical characteristics of microalga Scenedesmus quadricauda

Polystyrene nanoplastics were found to disrupt the physiology and biochemistry of freshwater microalgae, affecting photosynthesis, growth rates, and oxidative stress markers at environmentally relevant concentrations. The results highlight nanoplastics as a threat to phytoplankton, the base of freshwater food webs.

2022 Environmental Pollution 62 citations