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61,005 resultsShowing papers similar to Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms
ClearX-ray computed tomography: A novel non-invasive approach for the detection of microplastics in sediments?
Researchers tested whether X-ray computed tomography (CT scanning) can non-invasively detect microplastics in river sediment cores, finding it works well for particles 4 mm or larger but cannot resolve smaller microplastics below 125 μm due to resolution limits. Importantly, CT scanning also revealed sediment layering and structural features that affect where microplastics accumulate — information that is lost when sediment cores are physically extracted and processed by conventional methods. This non-destructive approach could improve how scientists study microplastic distribution in sediments.
Enhancing microplastic detection in biological tissue with x-ray computed tomography
This study tested X-ray computed tomography (CT scanning) as a way to detect microplastics in biological tissue, exploring whether this non-destructive imaging approach could improve on current methods that require chemically processing samples. A non-destructive technique would allow scientists to study microplastic distribution in tissues without destroying the sample, potentially enabling more detailed and repeatable analyses.
Enhancing microplastic detection in biological tissue with x-ray computed tomography
This study tested X-ray computed tomography (CT scanning) as a way to detect microplastics in biological tissue, exploring whether this non-destructive imaging approach could improve on current methods that require chemically processing samples. A non-destructive technique would allow scientists to study microplastic distribution in tissues without destroying the sample, potentially enabling more detailed and repeatable analyses.
Tissue Clearing To Localize Microplastics via Three-Dimensional Imaging of Whole Organisms
Researchers developed a tissue-clearing technique that renders whole organisms transparent after microplastic ingestion, allowing 3D fluorescence imaging to precisely locate unlabeled environmental microplastics inside an organism without destroying tissue. Unlike conventional digestion methods that lose spatial information, this approach preserves the organism's structure while a fluorescent dye selectively stains the plastics. This tool could substantially improve our understanding of where microplastics accumulate within living organisms and what tissues they affect.
Near-Infrared-II In Vivo Visualization and Quantitative Tracking of Micro/Nanoplastics in Fish
Scientists developed a new near-infrared imaging technique to track micro- and nanoplastics inside living zebrafish in real time, overcoming limitations of previous detection methods. They found that both sizes of plastic particles accumulated mainly in the gut, with microplastics concentrating more in the front sections and nanoplastics distributing more evenly. This tracking tool helps researchers better understand how plastic particles move through and accumulate in living organisms, which is essential for assessing the risks of microplastic exposure.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Visible Combined Near-Infrared in Situ Imaging Revealed Dynamic Effects of Microplastic Fibers and Beads in Zebrafish
Researchers used a combined visible and near-infrared imaging technique to track microplastic fibers and beads in live zebrafish in real time. They observed that microplastics were quickly ingested and could be retained briefly in the digestive tract before being eliminated. The study provides new insights into the dynamic behavior of microplastics inside living organisms and whether any tissue damage that occurs during transit can be reversed.
Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish
Researchers tracked the accumulation and distribution of fluorescent microplastics in early life stages of a freshwater organism, finding that microplastics were taken up and distributed across body tissues. The results help explain how microplastics accumulate in young aquatic organisms and potentially affect their development.
Can CT Imaging be Used to Investigate Microplastics in Sediments?
Researchers evaluated X-ray computed tomography (CT) as a non-destructive method for detecting microplastics in river sediment cores, testing the technique on layered, randomly spiked, and real environmental samples from the Thames estuary to assess its utility as an in-situ identification tool.
High resolution X-ray microtomography as a tool for observation and classification of individual microplastics
Researchers investigated X-ray microtomography (microCT) as a non-destructive tool for characterizing microplastics embedded in sediment, demonstrating that the technique could provide detailed internal and external morphological data to help classify individual particles based on structure and composition.
Exploring the impact of PVC and PVA microplastics on zebrafish tissue using multi-spectral imaging, Optical Coherence Tomography (OCT) and biospeckle OCT (bOCT)
Researchers used advanced optical imaging techniques to track how PVC and PVA microplastics accumulate in zebrafish tissues over a 21-day exposure period. They observed microplastic deposition primarily in the gill region, with PVC causing a more pronounced increase in biological activity compared to PVA. The study demonstrates the potential of non-invasive imaging methods for studying microplastic impacts on living organisms in real time.
Migration of MPs across biological barriers in zebrafish
The PlastSensing project developed new methods for detecting the migration of environmentally relevant microplastics across biological barriers in zebrafish tissue, using non-invasive visualization approaches that do not require tissue processing. The methods revealed plastic particle translocation pathways through zebrafish tissues at exposures relevant to real-world contamination levels.
Numerical Study towards In Vivo Tracking of Micro-/Nanoplastic Based on X-ray Fluorescence Imaging
Researchers conducted numerical simulations to evaluate X-ray fluorescence imaging as a method for tracking micro- and nanoplastic particles inside living organisms. The study found that by labeling plastic particles with detectable metal elements, it would be possible to map their distribution across organs with high spatial resolution. The approach could provide precise measurements of how plastic particles cross biological barriers and accumulate in tissues over time.
Migration of MPs across biological barriers in zebrafish
The PlastSensing project introduced new approaches for detecting microplastic migration across biological barriers in zebrafish, addressing the technical challenge of visualizing plastic particles in unprocessed biological tissue samples. New detection methods enabled tracking of microplastic movement through zebrafish tissues, providing insight into how particles translocate from gut to systemic circulation.
Development of Water Cluster-Secondary Ion Mass Spectrometry and Particle Induced X-ray Emission to Investigate Spatially Resolved Biological Responses to Nanopolystyrene in Zebrafish Larvae
Researchers developed advanced imaging techniques using water cluster-secondary ion mass spectrometry and particle-induced X-ray emission to study how nanoplastics interact with zebrafish larvae at the tissue level. They mapped the spatial distribution of elements and molecular changes in organs exposed to nanopolystyrene at environmentally realistic concentrations. The study provides new analytical methods for understanding how nanoplastics are taken up and distributed in living organisms, filling a critical gap in nanoplastic toxicology research.
Non-invasive detection and localization of microplastic particles in a sandy sediment by complementary neutron and X-ray tomography
Researchers used neutron and X-ray tomography — scanning technologies that see inside materials without cutting them open — to non-destructively detect and map microplastic particles inside sandy sediment samples, opening new possibilities for studying how microplastics move and accumulate in natural environments.
Challenges in assessing ecological and health risks of microplastics and nanoplastics: tracking their dynamics in living organisms
Researchers proposed a new method for tracking micro- and nanoplastics in living organisms using fluorescent monomers built directly into the plastic particles during synthesis. Current detection methods require destructive sampling and only provide static snapshots, missing the real-time movement of particles through biological systems. This fluorescent monomer approach is designed to enable continuous, stable imaging of plastic particles as they move through complex biological environments.
Harnessing PET to track micro- and nanoplastics in vivo
This study explores the use of positron emission tomography (PET) imaging to track micro- and nanoplastic particles in living organisms. Researchers developed methods to radiolabel plastic particles, enabling accurate determination of how these pollutants move through the body, which is critical for understanding the health effects of chronic microplastic exposure.
An end to the controversy over the microscopic detection and effects of pristine microplastics in fish organs
Researchers conducted feeding experiments with zebrafish to resolve conflicting findings about microplastic detection and effects in fish. They found that zebrafish generally recognize plastic particles as inedible but ingest them when mixed with food, and effectively eliminate most particles within 24 hours, though larger particles take longer to clear. The study demonstrates using confocal microscopy to track plastic microbead uptake and translocation in fish for the first time, while finding no histopathological damage from pristine microplastic ingestion.
In vivo visualization of environmentally relevant microplastics and evaluation of gut barrier damages in Artemia franciscana
Researchers developed non-destructive imaging methods to visualize the uptake of environmentally relevant irregularly-shaped microplastics in brine shrimp. The study found that high-density polyethylene particles accumulated in the gut and caused barrier damage, providing direct visual evidence of how realistic microplastic shapes affect small aquatic organisms.
Development of a toolbox for the analysis of microplastic-tissue interactions in two benthic freshwater organisms
Researchers developed a histological toolbox to analyze microplastic-tissue interactions in two benthic freshwater invertebrates, addressing the methodological gap in available protocols for detecting whether ingested microplastics simply pass through the gut or accumulate at specific tissue zones and translocate into organism tissues.
In situ imaging of microplastics in living organisms based on mass spectrometry technology
Researchers reviewed mass spectrometry-based imaging techniques for detecting microplastics inside living organisms, comparing different ion source methods for their ability to visualize plastic particles in biological tissue. They found that these techniques can provide both spatial distribution maps and chemical composition analysis of microplastics at high resolution. The study suggests that mass spectrometry imaging could become a powerful tool for understanding how microplastics accumulate and distribute within living systems.
Development of a toolbox for the analysis of microplastic-tissue interactions in two benthic freshwater organisms
Researchers developed and validated a histological toolbox for detecting microplastic-tissue interactions in two benthic freshwater organisms, creating protocols to determine whether ingested particles pass through the gut, accumulate at tissue sites, or translocate into body tissues following exposure to environmentally relevant microplastic concentrations.
Detection of microplastics in fish using computed tomography and deep learning
CT scanning combined with deep learning neural networks enabled non-destructive, automated detection and localization of microplastics in fish with high accuracy, overcoming the contamination risk and time-consuming nature of conventional dissection-based methods.