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Papers
61,005 resultsShowing papers similar to Efficient and Scalable Detection of Microplastics in Drinking Water Using Fluorescence High‐Content Imaging
ClearA new method for counting and sizing microplastic particles in water: investigating the presence of microplastics in Iranian bottled water
Researchers developed a novel method for counting and sizing microplastic particles in bottled water using Nile Red fluorescent staining combined with direct microscopic counting. Testing Iranian bottled water brands revealed widespread microplastic contamination, with the new method providing a faster and more accessible alternative to spectroscopic identification for routine bottled water monitoring.
Quantitative image analysis of microplastics in bottled water following Nile Red staining and fluorescence microscopy
Researchers developed and validated an integrated method for quantifying microplastics in bottled water using Nile Red staining, fluorescent microscopy, and automated image analysis with partial filter interrogation to boost analysis throughput. The method demonstrated high sensitivity for sizing microplastics down to 10 micrometers, with a limit of detection of 1.1 ppb, limit of quantification of 3.4 ppb, linearity between 10 ppb and 1.5 ppm (R2 = 0.99), and repeatability of 11-12% RSD.
Rapid and reliable detection of microplastics in drinking water using fluorescence microscopy
Researchers developed a fluorescence-based method for rapid detection and quantification of microplastics in drinking water, addressing the need for faster and more practical monitoring tools. The method achieved high sensitivity and allowed polymer discrimination without requiring expensive spectroscopic instrumentation.
Optimizing the Concentration of Nile Red for Screening of Microplastics in Drinking Water
This study optimized the concentration of Nile red fluorescent dye for pre-screening microplastics in drinking water, establishing a protocol that balances detection sensitivity with background fluorescence to improve accuracy in identifying plastic particles.
Rapid and reliable detection of microplastics in drinking water using fluorescence microscopy
This study developed a rapid and reliable fluorescence-based method for detecting microplastics in drinking water, addressing the need for faster alternatives to time-consuming conventional analytical approaches. The method demonstrated high sensitivity and specificity for common plastic polymers in drinking water matrices.
Advances in analysis of microplastics in drinking water treatment plants. Fluorescence techniques using iDye Pink
Microplastics between 0.1 and 5 mm were detected at multiple stages of three drinking water treatment plants, with Nile Red fluorescence staining and spectroscopic techniques showing that while treatment reduces MP loads, particles persist into final treated water.
Nile Red staining for nanoplastic quantification: Overcoming the challenge of false positive counts due to fluorescent aggregates
Researchers refined a Nile Red dye-based method to count nanoplastic particles in water by reducing dye concentration to near-nanomolar levels, minimizing false positives from dye aggregates that mimic plastic particles. Using this approach they detected approximately 250 nanoplastics per nanoliter in water from plastic bottles and cartons — roughly ten times more than in tap water.
Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry
Scientists developed an improved method for staining microplastics with a fluorescent dye (Nile red) that embeds the dye inside the plastic particles rather than just coating the surface, resulting in much brighter and more reliable detection. Combined with high-speed flow cytometry, the technique can rapidly count microplastic particles smaller than 10 µm in environmental water samples with recovery rates above 99%. Faster and more accurate counting methods like this are important for scaling up microplastic monitoring across many water sources.
Real-Time Quantification of Microplastics in Aquatic Systems via Fluorescence Microscopy
Researchers developed a real-time fluorescence microscopy method capable of quantifying microplastics in aquatic systems with high precision, providing a faster and more accessible tool for monitoring microplastic contamination in drinking water reservoirs.
Identifying microplastic contamination in drinking water: analysis and evaluation using spectroscopic methods
Researchers developed analytical methods to identify and quantify microplastic contamination in drinking water, evaluating extraction efficiency and detection accuracy across different water types and plastic particle sizes. The study assessed health implications based on measured plastic loads in treated water.
What's in your water? A comparative analysis of micro- and nanoplastics in treated drinking water and bottled water
Researchers developed a novel method to detect both micro- and nanoplastics in drinking water using scanning electron microscopy and advanced infrared spectroscopy capable of identifying particles as small as 300 nanometers. The study found that bottled water had significantly higher concentrations of plastic particles than treated tap water, particularly for smaller micro- and nanoplastics, with polyamide, PET, and polyethylene being the most common polymers.
Smaller-sized micro-plastics (MPs) contamination in single-use PET-bottled water in Thailand.
This study quantified microplastics in ten brands of single-use PET-bottled water sold in Thailand, finding an average of 140 particles per liter using fluorescent staining. The results add to global evidence that bottled water is a consistent route of human microplastic exposure and suggest that plastic packaging is a likely contamination source.
Characteristics of nano-plastics in bottled drinking water.
Researchers detected nanoplastics in commercially bottled drinking water using novel nanoparticle analysis techniques, finding particles in the nanometer size range in multiple brands. These findings are concerning because nanoplastics are thought to be more biologically active than larger microplastics and can more easily cross biological barriers in the body.
Detecting and Quantifying Microplastics in Bottled Water using Fluorescence Microscopy: A New Experiment for Instrumental Analysis and Environmental Chemistry Courses
This educational lab exercise teaches students to detect and count microplastics in bottled water using fluorescence microscopy and Nile Red staining. The exercise demonstrates that bottled water is a real exposure source and builds scientific literacy around microplastic contamination.
Detection of microplastics particles in the aquatic environment by staining
Using a simple Nile red fluorescent staining method and a standard fluorescence microscope, researchers detected microplastics in both surface reservoir water and treated drinking water before it entered city supply systems in two Russian industrial cities. The finding that microplastics survive conventional water treatment underlines the need for official monitoring standards in Russia and, more broadly, raises questions about the extent to which drinking water is a route of human microplastic exposure worldwide.
Release Characteristics of Small-Sized Microplastics in Bottled Drinks Using Flow Cytometry Sorting and Nile Red Staining
Researchers used flow cytometry combined with fluorescent dye staining to detect very small microplastics released from PET drinking bottles under different environmental conditions. They found that sunlight exposure and alkaline conditions significantly increased the release of particles in the 1-5 micrometer size range. The study suggests that outdoor use and storage conditions for bottled drinks can substantially influence microplastic contamination levels.
Quantitative image analysis of microplastics in bottled water using Artificial Intelligence
Researchers developed an artificial intelligence-based image analysis approach to quantitatively detect microplastics in bottled water, addressing the absence of validated analytical methods and enabling more reliable assessment of consumer exposure to microplastic contamination.
A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red
Researchers developed a rapid fluorescent screening method using Nile Red dye to detect and quantify microplastics in environmental samples, finding it significantly faster than conventional methods while maintaining reasonable accuracy.
Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
Microplastics in beverage containers were quantified by count, size, and morphology to characterize a potential route of human exposure to these particles. The study found microplastics across beverage container types, providing controlled quantitative data on how many particles consumers may ingest through drinks.
Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost- and time-effective technique
Researchers developed a semi-automated, cost-effective method for microplastic detection using Nile red fluorescent staining, showing it can significantly reduce the time and expense of identifying microplastics compared to traditional spectroscopic approaches.
First evidence of microplastics and their characterization in bottled drinking water from a developing country
Scientists in a developing country analyzed 10 brands of bottled drinking water and found microplastics in all samples, with concentrations varying by brand. The most common particles were polyethylene and polypropylene fragments and fibers, likely originating from the plastic bottle caps and packaging. This study adds to evidence that bottled water is a significant source of daily microplastic intake for people worldwide.
Insights into Anthropogenic Micro- and Nanoplastic Accumulation in Drinking Water Sources and Their Potential Effects on Human Health
This review examines the growing body of evidence on micro- and nanoplastic contamination in drinking water sources, including both tap water and bottled water intended for human consumption. Researchers summarize the potential health effects of exposure through ingestion, inhalation, and skin contact, noting that current detection methods struggle with particles smaller than 10 micrometers. The study highlights the need for improved analytical tools and further research to fully understand the health implications of plastic particles in drinking water.
A solution for controling microplastics in drinking water
Researchers developed and tested a system for controlling microplastic contamination in drinking water, reporting on removal efficiency at levels relevant to public health. The approach offered effective microplastic reduction from drinking water sources including tap and bottled water.
First assessment of occurrence, characteristics and human exposure of microplastics in bottled drinking water, Nepal
Researchers conducted the first assessment of microplastics in bottled drinking water from Nepal's mountain, hill, and Terai regions, finding microplastics in all 35 samples tested (mean 118 items/L), with fiber-shaped particles dominating at 71.76%, establishing a baseline for this developing-country context.