Article
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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Detection Methods
Food & Water
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Optimizing the Concentration of Nile Red for Screening of Microplastics in Drinking Water
ACS ES&T Water2023
22 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 45
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0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Laura M. Hernandez,
Laura M. Hernandez,
Jeffrey M. Farner,
Jeffrey M. Farner,
Sara Matthews,
Sara Matthews,
Laura M. Hernandez,
Laura M. Hernandez,
Nathalie Tufenkji
Laura M. Hernandez,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Heidi Jahandideh,
Nathalie Tufenkji
Nathalie Tufenkji
Jeffrey M. Farner,
Jeffrey M. Farner,
Jeffrey M. Farner,
Jeffrey M. Farner,
Jeffrey M. Farner,
Jeffrey M. Farner,
Dominique Claveau-Mallet,
Dominique Claveau-Mallet,
Dominique Claveau-Mallet,
Jeffrey M. Farner,
Nathalie Tufenkji
Laura M. Hernandez,
Nathalie Tufenkji
Dominique Claveau-Mallet,
Jeffrey M. Farner,
Laura M. Hernandez,
Laura M. Hernandez,
Dominique Claveau-Mallet,
Nathalie Tufenkji
Laura M. Hernandez,
Sara Matthews,
Jeffrey M. Farner,
Jeffrey M. Farner,
Laura M. Hernandez,
Jeffrey M. Farner,
Jeffrey M. Farner,
Nathalie Tufenkji
Jeffrey M. Farner,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Laura M. Hernandez,
Nathalie Tufenkji
Sara Matthews,
Sara Matthews,
Mira Okshevsky,
Jeffrey M. Farner,
Jeffrey M. Farner,
Jeffrey M. Farner,
Dominique Claveau-Mallet,
Jeffrey M. Farner,
Jeffrey M. Farner,
Nathalie Tufenkji
Nathalie Tufenkji
Jeffrey M. Farner,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Dominique Claveau-Mallet,
Dominique Claveau-Mallet,
Heidi Jahandideh,
Jeffrey M. Farner,
Laura M. Hernandez,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Sara Matthews,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Laura M. Hernandez,
Nathalie Tufenkji
Ranjan Roy,
Nathalie Tufenkji
Nathalie Tufenkji
Ranjan Roy,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Varoujan A. Yaylayan,
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Nathalie Tufenkji
Summary
This study optimized the concentration of Nile red fluorescent dye for pre-screening microplastics in drinking water, establishing a protocol that balances detection sensitivity with background fluorescence to improve accuracy in identifying plastic particles.
Study Type
Environmental
Increasing concern regarding the presence of microplastics in drinking water has led to a growing number of studies aimed at quantifying microplastics in water. In this work, we present an optimized procedure for the use of Nile red (NR) as a fluorescent staining agent for pre-screening of microplastics in bottled water. Positive and negative control experiments with NR concentrations ranging from 0.001 to 10 mg/L showed that the appropriate NR concentration is an important factor in obtaining representative particle counts. Non-optimized staining concentrations led to underestimation or overestimation of the particle count. In this study, the optimized NR staining concentration was found to be 0.1 mg/L. This method was successfully used to screen particles in seven different brands of bottled water, consisting of both still and carbonated water, in both plastic and glass bottles. Particles larger than 100 μm were chemically characterized using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). Sixty-seven percent of these particles pre-screened with NR were confirmed to be polymers. Particles smaller than 100 μm were qualitatively analyzed using pyrolysis coupled with gas chromatography and mass spectroscopy (Py-GC-MS). Analysis of polymers between ∼5 and 100 μm using Py-GC-MS confirmed that this smaller fraction generally mirrors the FTIR results for particles larger than l00 μm.