Papers

61,005 results
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Article Tier 2

Extraction and identification of microplastics from mussels: Method development and preliminary results

Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.

2021 Italian Journal of Food Safety 19 citations
Article Tier 2

DEVELOPMENT OF A NOVEL PROTOCOL FOR THE EXTRACTION OF SMALL MICROPLASTICS (1-5 µm) FROM BIOLOGICAL TISSUES

Researchers developed a novel extraction protocol to isolate and quantify small microplastics (1–5 µm) from biological tissues, addressing a major gap in marine contamination studies. The method improves detection of these hard-to-analyze particles, which are more likely to penetrate cells and accumulate in organisms.

2025 Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale
Article Tier 2

Microplastic quantification in Sabellaria reefs: a validated protocol for extraction from biogenic agglutinated matrices

Scientists developed a new method to accurately measure tiny plastic particles trapped in underwater reefs built by marine worms. These reef structures act like filters that collect microplastics from ocean water, which can then enter the food chain when other sea creatures eat organisms living on the reefs. This improved testing method will help researchers better track plastic pollution in coastal waters and understand how it might affect seafood that people eat.

2026 Environmental Science and Pollution Research
Article Tier 2

Microplastics extraction from oyster tissue v1

Researchers developed and validated a protocol for extracting microplastics from oyster tissue, addressing the challenges of isolating plastic particles from a complex biological matrix that includes lipids, proteins, and mineral content. The method enables reliable quantification of microplastic contamination in bivalves — a widely consumed seafood and established bioindicator of coastal pollution — supporting standardised monitoring of microplastic uptake in marine food species.

2024
Article Tier 2

Microplastic in marine environment: reworking and optimisation of two analytical protocols for the extraction of microplastics from sediments and oysters

Researchers improved existing protocols for extracting microplastics from both marine sediments and oysters, making the NOAA protocol more versatile and extending it to PET extraction. Validated extraction methods are essential for producing consistent, comparable data on microplastic contamination in seafood.

2020 MethodsX 40 citations
Article Tier 2

Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat

Researchers optimized an analytical protocol for extracting microplastics from seafood samples with varying fat content, addressing a key methodological challenge in accurately quantifying microplastic contamination in marine food sources given the ubiquitous presence of plastic particles smaller than 5 mm in marine environments.

2022 2 citations
Article Tier 2

A new analytical approach for monitoring microplastics in marine sediments

Researchers developed a new analytical approach for monitoring microplastics specifically in marine sediments, improving extraction and identification steps to enable more reliable and standardized environmental monitoring of seafloor contamination.

2013 Environmental Pollution 1349 citations
Article Tier 2

Methodology for microplastics quantification in clams

Researchers developed a simplified methodology for extracting and quantifying microplastics from clam tissue, addressing the need for standardized protocols to assess microplastic accumulation in seafood that poses direct human exposure risk when consumed whole.

2022 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Improved microplastic processing from complex biological samples using a customized vacuum filtration apparatus

Researchers developed a customized vacuum filtration apparatus to improve the processing of microplastics from complex biological marine samples, addressing longstanding methodological barriers in accurately separating and quantifying particles smaller than 5 mm. The system aims to reduce contamination and sample loss that have hindered standardization across microplastic monitoring studies in both abiotic and biotic compartments.

2022 Limnology and Oceanography Methods 10 citations
Article Tier 2

Marine microplastics - Method development for detection of plastic particles from sea water down to 10 μm

Researchers developed and tested methods to detect plastic particles down to 10 micrometers from seawater samples, addressing a key technical challenge in microplastic research. Better analytical methods that can detect smaller particles are essential for understanding the full extent of microplastic pollution and its ecological effects.

2014 DTU Sustain Conference 2014
Article Tier 2

A high-performance protocol for extraction of microplastics in fish

Researchers developed and tested a high-performance protocol for extracting microplastics from fish tissue, achieving high recovery rates and providing a standardized approach for seafood contamination monitoring.

2016 The Science of The Total Environment 689 citations
Article Tier 2

Investigation of Detection Method for Nanoplastics in Shellfish

Researchers investigated detection methods for nanoplastics in shellfish, evaluating analytical techniques capable of identifying and quantifying nanoscale plastic particles in bivalve tissues. The study addresses the methodological challenges of isolating and characterizing nanoplastics from complex biological matrices.

2024 Taipei Medical University Repository
Article Tier 2

Optimization of Extraction Method for Microplastic from Invertebrates Using Pancreatic Enzymes

Researchers optimized a method for extracting microplastics from invertebrates using pancreatic enzymes, which digest biological tissue without degrading plastic polymers. Improved extraction methods are essential for accurately measuring microplastic contamination in organisms and for assessing exposure levels across food chains.

2019 Theseus (Ammattikorkeakoulujen)
Article Tier 2

Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus

This study evaluated and optimized laboratory extraction methods for isolating and identifying microplastics from the tissues of green mussels, which accumulate plastic particles through their filter-feeding habits. Reliable extraction methods are essential for accurately quantifying microplastic contamination in shellfish consumed by humans.

2021 ACADEMIA JOURNAL OF BIOLOGY 3 citations
Article Tier 2

Microplastics in seafood: Benchmark protocol for their extraction and characterization

Researchers developed a benchmark protocol for extracting and characterizing microplastics from seafood samples, providing standardized methods to improve consistency and comparability across studies measuring human dietary exposure.

2016 Environmental Pollution 894 citations
Article Tier 2

Screening for microplastics in sediment, water, marine invertebrates and fish: Method development and microplastic accumulation

Researchers developed improved methods for extracting microplastics from biological samples and sediments, using enzymatic digestion that achieved 97% particle recovery without damaging the plastics. When applied to field samples from the North Sea and Swedish coast, they found microplastics in 8 of 9 invertebrate species tested and in 68% of brown trout. Mussel tissue contained roughly a thousand times more microplastic particles per kilogram than surrounding sediment or water.

2017 Marine Pollution Bulletin 539 citations
Article Tier 2

Simultaneous Determination of Small Microplastics' Size, Type, Charge, Number and Mass Concentration by Machine-Learning Driven Single-Particle Sensing

Scientists developed a new method that can identify and measure tiny plastic particles (microplastics) in the environment much more precisely than before, determining their size, type, and amount all at once. This breakthrough could help us better understand how these plastic pollutants move through our environment and potentially affect human health. The technology represents a major step forward in tracking microplastic contamination, which is increasingly found in our food, water, and air.

2026
Article Tier 2

Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.

2016 Environmental Toxicology and Chemistry 317 citations
Article Tier 2

New techniques for the detection of microplastics in sediments and field collected organisms

Researchers developed new techniques for detecting microplastics in sediment samples and for collecting particles in the field, improving the reliability and sensitivity of methods used to monitor environmental microplastic contamination.

2013 Marine Pollution Bulletin 971 citations
Article Tier 2

Improved Raman spectroscopy-based approach to assess microplastics in seafood

Researchers developed an improved Raman spectroscopy protocol for assessing microplastics in seafood, using green-lipped mussels and Japanese jack mackerel as test models. The study identified key methodological challenges in sample preparation and analysis, and proposed solutions that improve the accuracy and efficiency of microplastic identification in food products.

2021 Environmental Pollution 69 citations
Article Tier 2

Oil extraction following digestion to separate microplastics from mussels

This study compared several chemical digestion and extraction methods for isolating microplastics from mussel tissue, finding that hydrogen peroxide digestion followed by oil-based extraction achieved over 95% recovery across multiple plastic types. Optimized extraction methods are essential for accurately detecting microplastic contamination in shellfish that humans consume.

2021 Chemosphere 15 citations
Article Tier 2

Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples

A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.

2019 Environmental Toxicology and Chemistry 46 citations
Article Tier 2

Detection, counting and characterization of nanoplastics in marine bioindicators: a proof of principle study

Researchers demonstrated a proof-of-concept workflow for detecting and counting nanoplastic particles (below 1 µm) in marine invertebrate tissues using electron microscopy and spectroscopic confirmation, finding nanoplastics in marine bioindicator species and establishing a methodology for future monitoring programs.

2021 Microplastics and Nanoplastics 46 citations
Article Tier 2

Validation of an extraction method for microplastics from human materials

Researchers validated an extraction method for isolating microplastics from human and animal tissues, confirming that the procedure does not cause significant alteration to the plastic particles themselves.

2019 Clinical Hemorheology and Microcirculation 22 citations