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61,005 resultsShowing papers similar to Nile Red Staining as a Subsidiary Method for Microplastic Quantifica-tion: A Comparison of Three Solvents and Factors Influencing Application Reliability
ClearNile Red Staining as a Subsidiary Method for Microplastic Quantifica-tion: A Comparison of Three Solvents and Factors Influencing Application Reliability
This study evaluated Nile Red fluorescent staining as a method for quantifying microplastics in environmental samples, comparing it to traditional identification techniques. The approach can help distinguish microplastics from organic particles more quickly and cost-effectively, supporting higher-throughput microplastic analysis in environmental monitoring programs.
Dyeing to Know: Harmonizing Nile Red Staining Protocols for Microplastic Identification
Researchers systematically evaluated eight carrier solvents for Nile Red fluorescence staining to improve microplastic identification and classification. The study identified an acetone-water mixture as the optimal solvent, balancing strong fluorescence performance with minimal polymer degradation, and demonstrated that Fenton oxidation effectively eliminated false-positive fluorescence from natural organic materials.
Dyeing to Know: Optimizing Solvents for Nile Red Fluorescence in Microplastics Analysis
Researchers investigated how the choice of solvent affects Nile Red fluorescence staining for microplastic identification, optimizing solvent conditions to improve the reliability of fluorescence-based classification of microplastic polymer types in environmental samples.
Analyzing microplastics with Nile Red: Emerging trends, challenges, and prospects
This review evaluates the Nile Red staining technique as an analytical method for identifying and quantifying microplastics in environmental samples. The study concludes that while Nile Red has emerged as a viable low-cost alternative to visual identification for microplastics research, not everything that fluoresces is plastic, so additional spectroscopic analysis is needed to validate results.
Dyeing to Know: Harmonising Nile Red Staining Protocols for Microplastic Identification
Researchers tested eight carrier solvents for Nile Red fluorescence staining of ten common microplastic polymer types to identify which combinations best distinguish MPs. Carrier solvent choice significantly affected fluorescence behavior and classification accuracy, identifying specific solvent-polymer combinations that optimize MP identification—a step toward harmonizing the widely used but unstandardized Nile Red staining protocol.
Dyeing to Know: Optimizing Solvents for Nile Red Fluorescence in Microplastics Analysis
Researchers investigated how solvent choice influences Nile Red fluorescence staining for microplastic detection, optimizing conditions for polarity-dependent fluorescence to enable more accurate polymer classification in large-scale environmental microplastic sampling.
Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost- and time-effective technique
Researchers developed a semi-automated, cost-effective method for microplastic detection using Nile red fluorescent staining, showing it can significantly reduce the time and expense of identifying microplastics compared to traditional spectroscopic approaches.
Exploring the Efficacy of Nile Red in Microplastic Quantification: A Costaining Approach
This study assessed the effectiveness of Nile Red, a fluorescent dye commonly used to detect microplastics, by comparing it with other staining approaches and evaluating detection accuracy. The research found that costaining strategies and careful protocol standardization can improve the reliability of Nile Red-based microplastic quantification.
Characterization of Nile Red-Stained Microplastics through Fluorescence Spectroscopy
Researchers developed an improved method for characterizing microplastics using Nile Red fluorescent staining combined with fluorescence spectroscopy. They found that different plastic polymers produce distinct fluorescent signatures when stained, enabling more reliable identification of plastic types. The technique offers a faster and more affordable alternative to traditional microplastic detection methods, which could help scale up environmental monitoring efforts.
Identification and quantification of microplastics using Nile Red staining
Researchers tested Nile Red staining as a method for identifying and quantifying microplastics in environmental samples, finding it useful for rapid screening but noting limitations in distinguishing plastics from non-plastic particles.
Comprehensive assessment of factors influencing Nile red staining: Eliciting solutions for efficient microplastics analysis
Researchers conducted a comprehensive assessment of Nile red staining for microplastic analysis and found that wavelength, temperature, hydrogen peroxide treatment, NaCl addition, and plastic polymer type all significantly influence staining efficiency, proposing solutions to improve detection accuracy.
Nile red staining in microplastic analysis—proposal for a reliable and fast identification approach for large microplastics
Researchers tested Nile red staining with UV light photography as a rapid and reliable method for identifying large microplastics in environmental samples, finding it comparable to fluorescence microscopy-based staining and confirmed by μ-Raman spectroscopy.
Quantitative and Qualitative Differences of Common Microplastic Detection Procedures: Nile Red- assisted Fluorescence Microscopy and Confocal Micro-Raman Spectroscopy
Researchers compared Nile Red-assisted fluorescence microscopy and confocal micro-Raman spectroscopy for microplastic detection, finding an overall percentage difference of 421% between methods, with better agreement at smaller particle sizes and Raman spectroscopy offering superior ability to distinguish microplastics from organic matter.
Nile Red staining for the detection of microplastics: a comprehensive study on the emission spectra
This study systematically characterized how Nile Red fluorescence spectra vary across different polymer types, pigments, weathering states, and surface roughness, providing a more comprehensive reference for using Nile Red staining to identify microplastics in environmental samples.
Optimization of sample preparation, fluorescence- and Raman techniques for environmental microplastics
Researchers optimized methods for preparing and analyzing environmental microplastic samples using fluorescence staining with Nile Red dye and Raman spectroscopy. The study found that while fluorescence can broadly categorize plastics as polar or non-polar, Raman spectroscopy with a deep-UV laser was needed to reliably identify all polymer types, including those pigmented with carbon black.
Determination of microplastics in sediment by Nile red dye
Researchers evaluated Nile red dye staining combined with fluorescent microscopy, FTIR spectroscopy, and XRD analysis for identifying microplastics in Sava River sediment, finding the method useful for increasing particle visibility but insufficiently reliable on its own for complete polymer identification, with coagulation artifacts observed post-staining.
Exploring Nile Red staining as an analytical tool for surface-oxidized microplastics
Scientists evaluated Nile Red, a fluorescent dye commonly used to detect microplastics, and found it works differently depending on whether microplastics have been weathered by the environment. Surface oxidation from aging in the environment changes how well the dye sticks to plastics, which means current detection methods may be undercounting weathered microplastics in environmental samples.
Suitability of Nile Red Dye for In-Situ Microplastic Detection
This thesis evaluated the suitability of Nile Red fluorescent dye for detecting microplastics in environmental samples, examining its effectiveness and limitations for field use. Accurate and affordable detection methods are essential for tracking plastic pollution in oceans and waterways.
Particle size-dependent quantitative and qualitative differences of common microplastic detection procedures: Nile Red-assisted fluorescence microscopy and confocal micro-Raman spectroscopy
Researchers compared confocal micro-Raman spectroscopy and Nile Red-assisted fluorescence microscopy for detecting microplastics across different size classes. Both methods delivered better agreement for larger particles (>100 µm), with particle size significantly influencing the percentage differences between methods, underscoring the need for size-specific method validation.
Modification of a Nile Red Staining Method for Microplastics Analysis: A Nile Red Plate Method
Researchers developed a modified Nile Red staining method — the 'Nile Red plate method' (NR-P) — in which plates are pre-coated with Nile Red dye rather than staining microplastics directly, overcoming limitations of the standard method when organic matter residues interfere with particle staining. They optimised the NR solution concentration at 1000 mg/L and confirmed the method's effectiveness across multiple common polymer types.
Labeling Microplastics with Fluorescent Dyes for Detection, Recovery, and Degradation Experiments
Researchers optimized fluorescent dye staining protocols for labeling 17 different plastic polymer types using four textile dyes and Nile red for detection, recovery, and degradation experiments. Dye performance varied significantly by polymer type and staining conditions, and standardized protocols were recommended to improve comparability across microplastic studies.
Exploring the potential of photoluminescence spectroscopy in combination with Nile Red staining for microplastic detection
Researchers explored photoluminescence spectroscopy combined with Nile Red staining as a cost- and time-efficient detection method for microplastics, evaluating improvements to existing fluorescence microscopy approaches for more reliable global monitoring of microplastic abundance.
The potential of fluorescent dyes—comparative study of Nile red and three derivatives for the detection of microplastics
Researchers compared Nile red and three newly developed fluorescent dye derivatives for staining microplastics, finding that the derivatives achieved greater selectivity for plastic particles and more intense fluorescence than standard Nile red, improving detection sensitivity.
Detection limits are central to improve reporting standards when using Nile red for microplastic quantification
Researchers evaluated the efficacy of Nile red fluorescent staining for quantifying microplastics, demonstrating that polymer-specific differences in fluorescent response create variable detection limits that undermine the comparability and reproducibility of MP quantification across studies. The authors call for standardized reporting of detection limits when using Nile red to improve data quality in microplastic research.