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Determination of microplastics in sediment by Nile red dye
Summary
Researchers evaluated Nile red dye staining combined with fluorescent microscopy, FTIR spectroscopy, and XRD analysis for identifying microplastics in Sava River sediment, finding the method useful for increasing particle visibility but insufficiently reliable on its own for complete polymer identification, with coagulation artifacts observed post-staining.
Mikroplastika predstavlja značajan okolišni problem i zahtijeva učinkovite metode identifikacije i uklanjanja. U ovom radu ispitivana je prisutnost i identifikacija mikroplastike u sedimentu rijeke Save metodama kao što su bojenje mikroplastike i čestica lakših od 1,65 g/cm3 Nile redom, nakon čega je slijedila analiza s lupom, fluorescentnom mikroskopijom, FTIR spektroskopijom i XRD analizom. Bojenje Nile redom se pokazalo korisnim jer čestice postaju uočljivije, iako sama metoda nije dovoljno pouzdana za potpunu identifikaciju plastike. Nakon bojenja zabilježena je pojava koagulacije pojedinih čestica (PVC-a i miješanih čestica ), dok se acetamid otopio te na njemu nije bilo moguće provesti ostatak postupaka, što ukazuje ograničenja metode. Rezultati su pokazali najjasnije obojenje i fluorescenciju za PVC, dok je PE pokazao slabije obojenje i fluorescenciju. Miješane čestice su srednje izražene. FTIR analizom, usporedbom spektara sa bazom podataka potvrđen je sastav PVC-a, PE-a, i miješanih čestica. XRD analizom sedimenta utvrđen je mineralni sastav koji odgovara području rijeke Save, u kojem dominira kvarc. Vlakna različitih boja i veličina uočena u uzorcima pomoću lupe, nakon što je izdvojena lakša i teža frakcija, upućuju na moguću prisutnost mikroplastike tekstilnog porijekla.
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