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Papers
20 resultsShowing papers similar to Evaluation and optimisation of sample preparation protocols suitable for the analysis of plastic particles present in seafood
ClearOptimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers optimized an analytical protocol for extracting microplastics from seafood samples with varying fat content, addressing a key methodological challenge in accurately quantifying microplastic contamination in marine food sources given the ubiquitous presence of plastic particles smaller than 5 mm in marine environments.
Microplastics in seafood: Benchmark protocol for their extraction and characterization
Researchers developed a benchmark protocol for extracting and characterizing microplastics from seafood samples, providing standardized methods to improve consistency and comparability across studies measuring human dietary exposure.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
Towards harmonised methods for microplastic analysis in food: development and optimisation for seafood products
Researchers evaluated and optimized methods for detecting and quantifying microplastics in the edible parts of seafood products, comparing enzymatic-alkaline digestion combined with filtration and oxidative treatment against other approaches for sensitivity, precision, and practical accessibility. The study aimed to contribute to method standardization, identifying the combined digestion protocol as most suitable for routine food control analysis.
Challenges in the blue economy: Methods for digesting and extracting microplastics from marine resources
Researchers reviewed methods for digesting and extracting microplastics from seafood and other marine products as part of addressing microplastic challenges in the blue economy. The review identified best-practice extraction protocols and highlighted the need for harmonized methods across laboratories.
Various Digestion Protocols Within Microplastic Sample Processing—Evaluating the Resistance of Different Synthetic Polymers and the Efficiency of Biogenic Organic Matter Destruction
Multiple organic matter digestion protocols used in microplastic sample preparation were systematically evaluated for their effectiveness in removing biogenic material while preserving plastic particles. The review helps standardize sample processing by identifying which digestion chemicals and conditions work best for different environmental matrices.
Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
Researchers developed and tested a new protocol for the extraction and characterization of microplastics from environmental samples, optimizing steps for recovery efficiency and polymer identification accuracy.
Oil extraction following digestion to separate microplastics from mussels
This study compared several chemical digestion and extraction methods for isolating microplastics from mussel tissue, finding that hydrogen peroxide digestion followed by oil-based extraction achieved over 95% recovery across multiple plastic types. Optimized extraction methods are essential for accurately detecting microplastic contamination in shellfish that humans consume.
Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers developed and optimized an analytical extraction protocol for microplastics from seafood samples with varying fat content, testing the method on fresh and canned fish and molluscs. They found that fat-rich matrices required additional lipid removal steps to achieve reliable extraction efficiency, and validated a protocol suitable for high-fat seafood that reduces matrix interference while maintaining microplastic recovery.
Microplastic in marine environment: reworking and optimisation of two analytical protocols for the extraction of microplastics from sediments and oysters
Researchers improved existing protocols for extracting microplastics from both marine sediments and oysters, making the NOAA protocol more versatile and extending it to PET extraction. Validated extraction methods are essential for producing consistent, comparable data on microplastic contamination in seafood.
Extraction method development for nanoplastics from oyster and fish tissues
Researchers developed a method for extracting nanoplastics from oyster and fish tissues using enzymatic digestion, sequential membrane filtration, and purification steps, addressing a critical methodological gap for assessing nanoplastic contamination in seafood.
Methodology optimization to quantify microplastic presence in planktonic copepods, chaetognaths and fish larvae
Researchers optimized a hydrogen peroxide digestion method for extracting and counting microplastics from zooplankton and fish larvae while preserving the physical and chemical integrity of the plastic particles, achieving over 85% recovery across 13 plastic types. Having a reliable, standardized method for quantifying microplastics in plankton is essential for accurately assessing how much plastic is entering marine food webs at the base of the food chain.
Optimization of a new multi-reagent procedure for quantitative mussel digestion in microplastic analysis
This study optimized a chemical digestion protocol for extracting microplastics from mussel tissue by testing combinations of multiple reagents, aiming to completely destroy biological material while preserving plastic particles for analysis. The optimized method improved microplastic recovery compared to single-reagent approaches. Accurate extraction methods are critical for reliably measuring microplastic contamination in mussels, a widely consumed shellfish and important food safety indicator.
Comparison of three digestion methods for microplastic extraction from aquaculture feeds
Researchers compared three digestion methods for extracting microplastics from aquaculture feed samples, evaluating their effectiveness and impact on plastic particle integrity. The study found that the choice of digestion protocol significantly affects microplastic recovery rates, highlighting the importance of method standardization for reliable contamination assessments in aquaculture products.
A high-performance protocol for extraction of microplastics in fish
Researchers developed and tested a high-performance protocol for extracting microplastics from fish tissue, achieving high recovery rates and providing a standardized approach for seafood contamination monitoring.
Developmentof Standardized Methods to Extract andDigest Microplastics in Environmental Samples
Researchers reviewed and compared extraction and digestion methods for isolating microplastics from environmental samples, finding that the lack of standardization across techniques is a major barrier to producing comparable results and calling for validated universal protocols.
Extraction and identification of microplastics from mussels: Method development and preliminary results
Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.
Improving the efficiency of post-digestion method in extracting microplastics from gastrointestinal tract and gills of fish
Researchers compared three post-digestion techniques for separating microplastics from fish tissue samples and found sieving and zinc chloride density separation both achieved over 95% efficiency without damaging most polymer types. The study provides practical guidance for improving the accuracy of microplastic detection in marine biological samples.
Microplastic extraction from digestive tracts of large decapods
This study developed and validated a method for extracting microplastics from the digestive tracts of large decapod crustaceans, enabling accurate quantification and polymer identification. The protocol minimizes contamination and is suitable for routine monitoring of seafood species.