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Papers
61,005 resultsShowing papers similar to DEVELOPMENT OF A NOVEL PROTOCOL FOR THE EXTRACTION OF SMALL MICROPLASTICS (1-5 µm) FROM BIOLOGICAL TISSUES
ClearValidation of an extraction method for microplastics from human materials
Researchers validated an extraction method for isolating microplastics from human and animal tissues, confirming that the procedure does not cause significant alteration to the plastic particles themselves.
Improved microplastic processing from complex biological samples using a customized vacuum filtration apparatus
Researchers developed a customized vacuum filtration apparatus to improve the processing of microplastics from complex biological marine samples, addressing longstanding methodological barriers in accurately separating and quantifying particles smaller than 5 mm. The system aims to reduce contamination and sample loss that have hindered standardization across microplastic monitoring studies in both abiotic and biotic compartments.
Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers optimized an analytical protocol for extracting microplastics from seafood samples with varying fat content, addressing a key methodological challenge in accurately quantifying microplastic contamination in marine food sources given the ubiquitous presence of plastic particles smaller than 5 mm in marine environments.
Optimisation of Small Microplastic Extraction and Quantification from Marine Tissues
Scientists developed a better way to find and count tiny plastic particles (called microplastics) inside sea creatures like mussels. This is important because these plastic pieces can build up in seafood that people eat, but until now it was hard for researchers to detect the smallest particles. The improved method will help scientists better track plastic pollution in our food chain and understand potential health risks.
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
Researchers developed and tested a new protocol for the extraction and characterization of microplastics from environmental samples, optimizing steps for recovery efficiency and polymer identification accuracy.
Solving a Sticky Situation: Microplastic Analysis of Lipid-Rich Tissue
Researchers developed and validated an optimized protocol for extracting and analyzing microplastics from lipid-rich biological tissues, addressing the challenge that standard enzymatic digestion methods leave fatty residues that interfere with microplastic detection and quantification.
A high-performance protocol for extraction of microplastics in fish
Researchers developed and tested a high-performance protocol for extracting microplastics from fish tissue, achieving high recovery rates and providing a standardized approach for seafood contamination monitoring.
Investigation of Detection Method for Nanoplastics in Shellfish
Researchers investigated detection methods for nanoplastics in shellfish, evaluating analytical techniques capable of identifying and quantifying nanoscale plastic particles in bivalve tissues. The study addresses the methodological challenges of isolating and characterizing nanoplastics from complex biological matrices.
A novel extraction protocol of nano-polystyrene from biological samples
Researchers developed a diatomite-based extraction protocol for isolating nano-polystyrene from biological samples, providing a method to quantify nanoplastics in tissues that had previously lacked reliable analytical tools.
Extraction of biodegradable microplastics from tissues of aquatic organisms
Researchers developed and validated a method for extracting biodegradable microplastics from tissues of aquatic organisms, finding that standard extraction protocols used for conventional plastics are insufficient and that enzymatic digestion combined with density separation is needed to recover these materials accurately.
Microplastics extraction from oyster tissue v1
Researchers developed and validated a protocol for extracting microplastics from oyster tissue, addressing the challenges of isolating plastic particles from a complex biological matrix that includes lipids, proteins, and mineral content. The method enables reliable quantification of microplastic contamination in bivalves — a widely consumed seafood and established bioindicator of coastal pollution — supporting standardised monitoring of microplastic uptake in marine food species.
Extraction and identification of microplastics from mussels: Method development and preliminary results
Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.
A review on methods for extracting and quantifying microplastic in biological tissues
This review evaluates the methods scientists use to extract and identify microplastics from biological tissues like fish, shellfish, and animal organs. Researchers found that different extraction approaches (using acids, bases, or enzymes) yield different results, and many studies report finding plastics at sizes that may be too large to have actually crossed biological barriers. The study calls for more standardized and biologically plausible methods to improve the reliability of tissue contamination research.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
Novel methodology for identification and quantification of microplastics in biological samples
Researchers validated a protocol for identifying and quantifying polyethylene microplastics in biological samples, finding that membrane filtration caused particle retention problems and that flow cytometry offered a more reliable alternative for analysis of biological digests.
Occurrence, Distribution, and Extraction Methods of Microplastics in Marine Organisms
This review synthesizes global data on microplastic occurrence and distribution across marine organisms, comparing the advantages and limitations of different extraction and identification methods used in the field.
Isolation of microplastics in biota-rich seawater samples and marine organisms
Researchers developed and tested methods for extracting microplastics from seawater samples rich in biological material and from the tissues of marine organisms. They found that enzymatic digestion was the most effective and least destructive approach for isolating microplastics from biological samples, outperforming acid and alkaline treatments. The study provides improved laboratory protocols that will help scientists more accurately measure microplastic contamination in marine environments and wildlife.
A Workflow for Assessing Particle Counts of Mixed Micro- and Nanoplastics in Exposed Laboratory Animals
Researchers developed a laboratory protocol for extracting and counting micro- and nanoplastic particles from mouse tissue samples. They achieved an 85% recovery rate for 2-micrometer particles and 30% for 0.1-micrometer particles, and demonstrated the method could detect differences in accumulation across different exposure levels. This workflow provides a foundation for future studies investigating how plastic particles accumulate in biological tissues.
Marine microplastics - Method development for detection of plastic particles from sea water down to 10 μm
Researchers developed and tested methods to detect plastic particles down to 10 micrometers from seawater samples, addressing a key technical challenge in microplastic research. Better analytical methods that can detect smaller particles are essential for understanding the full extent of microplastic pollution and its ecological effects.
Detection, counting and characterization of nanoplastics in marine bioindicators: a proof of principle study
Researchers demonstrated a proof-of-concept workflow for detecting and counting nanoplastic particles (below 1 µm) in marine invertebrate tissues using electron microscopy and spectroscopic confirmation, finding nanoplastics in marine bioindicator species and establishing a methodology for future monitoring programs.
Microplastic accumulation, depuration dynamics and localization in environmental compartments: combination of experimental set ups and field studies
Researchers used microcosm experiments to track microplastic accumulation, depuration, and tissue localization in marine organisms under environmentally relevant conditions. The study found that organisms can accumulate and partially depurate microplastics, with particle retention varying by tissue type and particle size.
Microplastics in seafood: Benchmark protocol for their extraction and characterization
Researchers developed a benchmark protocol for extracting and characterizing microplastics from seafood samples, providing standardized methods to improve consistency and comparability across studies measuring human dietary exposure.
Morphological and chemical characterization of nanoplastics in human tissue
Researchers developed methods to visualize and chemically characterize nanoplastics that have accumulated in human tissue samples. They were able to identify plastic particles smaller than one micrometer within tissue using advanced microscopy and spectroscopy techniques. The study provides some of the first direct evidence of nanoscale plastic accumulation in the human body, which is essential for designing future health effects research.
A new analytical approach for monitoring microplastics in marine sediments
Researchers developed a new analytical approach for monitoring microplastics specifically in marine sediments, improving extraction and identification steps to enable more reliable and standardized environmental monitoring of seafloor contamination.