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Extraction of biodegradable microplastics from tissues of aquatic organisms
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Researchers developed and validated a method for extracting biodegradable microplastics from tissues of aquatic organisms, finding that standard extraction protocols used for conventional plastics are insufficient and that enzymatic digestion combined with density separation is needed to recover these materials accurately.
Biodegradable plastics (BPs) have been given high hopes to substitute conventional plastics, but their biodegradation requires strict conditions. BPs can accumulate for a long time in the environment and even derive biodegradable microplastics (BMPs), thus threatening wildlife and ecosystems. However, no efficient method is available for extracting BMPs from organisms' tissues. This study used multi-criteria decision-making (MCDM) methods to comprehensively evaluate and optimize extraction protocols of five BMPs from economic aquatic species. Digestion time, digestion efficiency, mass loss, cost, polymer integrity and size change were selected as evaluating indictors. According to the screening results of MCDM methods, Pepsin+HO was selected as the optimal digestion method of BMPs because of its highest comprehensive score, which has high digestion efficiency (99.56%) and minimum plastic damage. Compared with olive oil, NaI is more suitable for separating BMPs from the digested residues. Furthermore, the combination of Pepsin+HO digestion and NaI density separation was used to extract all five kinds of BMPs from the bivalve, crab, squid, and crayfish tissues, and all the recovery rates exceeded 80%. These results suggest that the optimal protocol is practicable to extract various BMPs from various aquatic organisms.
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Researchers developed and tested methods for extracting microplastics from seawater samples rich in biological material and from the tissues of marine organisms. They found that enzymatic digestion was the most effective and least destructive approach for isolating microplastics from biological samples, outperforming acid and alkaline treatments. The study provides improved laboratory protocols that will help scientists more accurately measure microplastic contamination in marine environments and wildlife.
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
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Validation of microplastic sample preparation method for freshwater samples
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A straightforward method for microplastic extraction from organic-rich freshwater samples
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Researchers developed a streamlined method for extracting microplastics from organic-rich freshwater samples using centrifugation and enzymatic digestion. They found that the approach effectively concentrated microplastics while preserving their integrity and minimizing contamination, at lower cost than existing methods. The study offers a practical and accessible protocol for laboratories studying microplastic pollution in freshwater environments.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.