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61,005 resultsShowing papers similar to Assessment of microplastics using microfluidic approach
ClearA Low-Cost Microfluidic Method for Microplastics Identification: Towards Continuous Recognition
Researchers developed a low-cost 3D-printed microfluidic device combining Nile Red staining with continuous-flow processing to enable rapid, affordable microplastic identification, demonstrating performance comparable to conventional staining methods while supporting field-deployable monitoring.
A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red
Researchers developed a rapid fluorescent screening method using Nile Red dye to detect and quantify microplastics in environmental samples, finding it significantly faster than conventional methods while maintaining reasonable accuracy.
Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost- and time-effective technique
Researchers developed a semi-automated, cost-effective method for microplastic detection using Nile red fluorescent staining, showing it can significantly reduce the time and expense of identifying microplastics compared to traditional spectroscopic approaches.
Rapid detection of nanoplastics and small microplastics by Nile-Red staining and flow cytometry
Researchers developed a rapid method for detecting nanoplastics and small microplastics by combining Nile-Red fluorescent staining with flow cytometry. The technique can quantify plastic particles in the 0.6 to 15 micrometer range in just 90 seconds, which is hundreds of times faster than conventional spectroscopic methods. The approach showed high detection efficiency for polyethylene, polyvinylchloride, and polystyrene, offering a practical tool for environmental nanoplastic monitoring.
Identification and quantification of microplastics using Nile Red staining
Researchers tested Nile Red staining as a method for identifying and quantifying microplastics in environmental samples, finding it useful for rapid screening but noting limitations in distinguishing plastics from non-plastic particles.
Characterization of Nile Red-Stained Microplastics through Fluorescence Spectroscopy
Researchers developed an improved method for characterizing microplastics using Nile Red fluorescent staining combined with fluorescence spectroscopy. They found that different plastic polymers produce distinct fluorescent signatures when stained, enabling more reliable identification of plastic types. The technique offers a faster and more affordable alternative to traditional microplastic detection methods, which could help scale up environmental monitoring efforts.
Separation and flow cytometry analysis of microplastics and nanoplastics
Researchers improved a flow cytometry method for counting and separating microplastics and nanoplastics stained with a fluorescent dye called Nile Red. By adjusting the chemical solution used in detection, they reduced particle clumping and improved measurement accuracy for plastic particles across a range of sizes. The refined technique offers a faster and more reliable way to quantify plastic pollution in environmental and biological samples.
Development of an Inexpensive and Comparable Microplastic Detection Method Using Fluorescent Staining with Novel Nile Red Derivatives
Researchers developed an inexpensive fluorescent staining method using novel Nile Red derivatives for microplastic detection, validating it by measuring microplastics in German wastewater treatment plant effluent over one year with improved precision and selectivity.
Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry
Scientists developed an improved method for staining microplastics with a fluorescent dye (Nile red) that embeds the dye inside the plastic particles rather than just coating the surface, resulting in much brighter and more reliable detection. Combined with high-speed flow cytometry, the technique can rapidly count microplastic particles smaller than 10 µm in environmental water samples with recovery rates above 99%. Faster and more accurate counting methods like this are important for scaling up microplastic monitoring across many water sources.
Nile Red Staining as a Subsidiary Method for Microplastic Quantifica-tion: A Comparison of Three Solvents and Factors Influencing Application Reliability
This study evaluated Nile Red fluorescent staining as a method for quantifying microplastics in environmental samples, comparing it to traditional identification techniques. The approach can help distinguish microplastics from organic particles more quickly and cost-effectively, supporting higher-throughput microplastic analysis in environmental monitoring programs.
Exploring the potential of photoluminescence spectroscopy in combination with Nile Red staining for microplastic detection
Researchers explored photoluminescence spectroscopy combined with Nile Red staining as a cost- and time-efficient detection method for microplastics, evaluating improvements to existing fluorescence microscopy approaches for more reliable global monitoring of microplastic abundance.
Rapid detection of microplastic contamination using Nile red fluorescent tagging
Researchers developed a rapid microplastic detection method using Nile Red (NR) fluorescent staining combined with zinc chloride density-based extraction and filtration for analysis of coastal marine sediment samples. The approach was cross-validated against conventional light microscopy, demonstrating improved speed and sensitivity for identifying microplastics of various sizes in environmental sediment matrices.
Modification of fluorescence staining method for small-sized microplastic quantification: Focus on the interference exclusion and exposure time optimization
Researchers optimized a Nile Red/DAPI fluorescence co-staining method for quantifying small microplastics, identifying key interference factors and exposure time parameters that significantly improve accuracy of microplastic detection.
Preliminary Results From Detection of Microplastics in Liquid Samples Using Flow Cytometry
Researchers developed a novel flow cytometry approach for in-situ detection and quantification of microplastics in liquid samples using fluorescent staining, testing nine polymer types under controlled laboratory conditions. The method offers a high-throughput alternative to traditional time-consuming microplastic detection protocols that risk sample contamination.
A microfluidic approach for label-free identification of small-sized microplastics in seawater
Researchers developed a microfluidic approach for label-free identification of small microplastics in seawater, using impedance-based detection to distinguish different polymer types without chemical labeling, enabling faster and more practical environmental monitoring.
A new method for counting and sizing microplastic particles in water: investigating the presence of microplastics in Iranian bottled water
Researchers developed a novel method for counting and sizing microplastic particles in bottled water using Nile Red fluorescent staining combined with direct microscopic counting. Testing Iranian bottled water brands revealed widespread microplastic contamination, with the new method providing a faster and more accessible alternative to spectroscopic identification for routine bottled water monitoring.
Integrated sample processing and counting microfluidic device for microplastics analysis
Researchers developed an integrated microfluidic device that combines sample digestion, filtration, and counting processes for streamlined microplastic analysis. The study demonstrated that this automated approach can successfully quantify microplastics from both river water sediment and fish gastrointestinal tract samples, offering a faster and more accessible alternative to conventional time-consuming manual analysis methods.
Analyzing microplastics with Nile Red: Emerging trends, challenges, and prospects
This review evaluates the Nile Red staining technique as an analytical method for identifying and quantifying microplastics in environmental samples. The study concludes that while Nile Red has emerged as a viable low-cost alternative to visual identification for microplastics research, not everything that fluoresces is plastic, so additional spectroscopic analysis is needed to validate results.
Rapid methods for the quantification of ingested nano-and microplastics in marine fish by imaging flow cytometry
Researchers developed a rapid, high-throughput method using imaging flow cytometry to quantify nano- and microplastics ingested by marine fish. The optimized technique uses Nile Red fluorescent staining and morphology-based corrections to accurately count plastic particles, providing a faster and more reliable alternative to conventional detection methods for ecological risk assessments.
Towards a low-cost, rapid microplastic optical detection system using fluorescent staining through Nile Red for in situ ocean deployment
This study presents a proof-of-concept for a portable, low-cost microplastic detection device that uses fluorescent dye (Nile Red) and a simple optical sensor to detect plastic particles in water. The system produced a signal that scaled linearly with microplastic concentration in lab tests. Development of cheap, field-deployable sensors like this could dramatically improve our ability to monitor microplastic pollution in real time across oceans and waterways, where current lab-based methods are too expensive and slow for widespread use.
Flow Cytometry as a Rapid Alternative to Quantify Small Microplastics in Environmental Water Samples
Researchers developed a flow cytometry method using fluorescent staining to rapidly detect and quantify small microplastics (1-50 micrometers) in environmental water samples, achieving over 80% recovery rates and significantly reducing analysis time compared to traditional microscopy.
Facile detection of microplastics from a variety of environmental samples with conjugated polymer nanoparticles
Researchers developed a quick and straightforward method for detecting microplastics in environmental samples using fluorescent conjugated polymer nanoparticles. The technique can identify microplastic particles across a range of sample types without requiring complex laboratory equipment. This approach could make microplastic monitoring more accessible and practical for routine environmental testing.
Rapid detection and quantification of Nile Red-stained microplastic particles in sediment samples
Researchers developed a Nile Red staining method combined with automated fluorescence microscopy to rapidly detect and quantify microplastics in deep-sea sediment samples. The method significantly reduced analysis time compared to manual identification while maintaining accuracy, enabling higher-throughput monitoring of microplastic contamination in marine sediments.
Microfluidics-based electrophoretic capture and Raman analysis of micro/nanoplastics
Researchers developed a microfluidics-based electrophoretic capture system combined with Raman spectroscopy analysis to detect and characterize micro- and nanoplastics from aquatic ecosystems, exploiting differences in polymer composition to improve identification accuracy.