We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Development of an Inexpensive and Comparable Microplastic Detection Method Using Fluorescent Staining with Novel Nile Red Derivatives
AI summary Read the abstract
Researchers developed an inexpensive fluorescent staining method using novel Nile Red derivatives for microplastic detection, validating it by measuring microplastics in German wastewater treatment plant effluent over one year with improved precision and selectivity.
Fluorescent staining of microplastics as a detection method is consistently gaining importance in microplastics research, as it is fast, easy to use, and requires low technical effort. In this study, a complete procedure was developed, from sample collection to sample processing and detection, to measure microplastics with low cost and time requirements. The developed procedure was tested by measuring the microplastics in the effluent of a German wastewater treatment plant over a period of one year. The results show that the process is especially well suited to investigate temporal variations of microplastic contamination, which requires a large number of samples to be processed. Further, the precision and selectivity of the detection process could be improved by applying newly developed Nile red derivatives for fluorescent staining. A low budget modification of a microscope for fluorescent imaging is compared to a modification with precise optical bandpass filters. A script enabling automated microplastic detection and counting was developed, improving the accuracy and comparability of the process.
More Papers Like This
Standardized fluorescent staining method for fast and affordable microplastics monitoring and its application for a long-term monitoring of microplastics in the effluent of a German wastewater treatment plant.
AI summary Read the abstract
Researchers developed and validated a standardized fluorescent staining method using modified Nile red dye to rapidly detect microplastics in environmental samples at low cost, then applied it to monitor microplastic concentrations in wastewater treatment plant effluent over an extended period. Standardized, affordable detection methods are essential for the widespread long-term monitoring needed to track microplastic pollution trends.
The potential of fluorescent dyes—comparative study of Nile red and three derivatives for the detection of microplastics
AI summary Read the abstract
Researchers compared Nile red and three newly developed fluorescent dye derivatives for staining microplastics, finding that the derivatives achieved greater selectivity for plastic particles and more intense fluorescence than standard Nile red, improving detection sensitivity.
Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost- and time-effective technique
AI summary Read the abstract
Researchers developed a semi-automated, cost-effective method for microplastic detection using Nile red fluorescent staining, showing it can significantly reduce the time and expense of identifying microplastics compared to traditional spectroscopic approaches.
A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red
AI summary Read the abstract
Researchers developed a rapid fluorescent screening method using Nile Red dye to detect and quantify microplastics in environmental samples, finding it significantly faster than conventional methods while maintaining reasonable accuracy.
Identification and Quantification of Microplastics Using Nile Red Staining
AI summary Read the abstract
This study developed a rapid fluorescent staining method using the dye Nile Red to identify and quantify microplastics in environmental samples, offering an alternative to slower and more expensive spectroscopic techniques. Nile Red staining provides a faster way to differentiate microplastics from natural particles in large sample sets, improving throughput for environmental monitoring.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.