Article
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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Detection Methods
Policy & Risk
Remediation
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Development of an Inexpensive and Comparable Microplastic Detection Method Using Fluorescent Staining with Novel Nile Red Derivatives
Analytica—A Journal of Analytical Chemistry and Chemical Analysis2023
41 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 50
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0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Michael Sturm,
Michael Sturm,
Katrin Schuhen
Katrin Schuhen
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Anika Korzin,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Anika Korzin,
Katrin Schuhen
Michael Sturm,
Michael Sturm,
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Erika Myers,
Anika Korzin,
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Michael Sturm,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Erika Myers,
Erika Myers,
Erika Myers,
Erika Myers,
Erika Myers,
Erika Myers,
Erika Myers,
Anika Korzin,
Anika Korzin,
Dennis Schober,
Katrin Schuhen
Katrin Schuhen
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Erika Myers,
Katrin Schuhen
Anika Korzin,
Anika Korzin,
Erika Myers,
Erika Myers,
Erika Myers,
Anika Korzin,
Dennis Schober,
Dennis Schober,
Anika Korzin,
Dennis Schober,
Dennis Schober,
Michael Sturm,
Dennis Schober,
Anika Korzin,
Dennis Schober,
Dennis Schober,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Michael Sturm,
Anika Korzin,
Dennis Schober,
Dennis Schober,
Michael Sturm,
Erika Myers,
Katrin Schuhen
Katrin Schuhen
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Anika Korzin,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Katrin Schuhen
Katrin Schuhen
Anika Korzin,
Erika Myers,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Anika Korzin,
Anika Korzin,
Dennis Schober,
Katrin Schuhen
Anika Korzin,
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Erika Myers,
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Dennis Schober,
Dennis Schober,
Dennis Schober,
Katrin Schuhen
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Dennis Schober,
Katrin Schuhen
Dennis Schober,
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Katrin Schuhen
Summary
Researchers developed an inexpensive fluorescent staining method using novel Nile Red derivatives for microplastic detection, validating it by measuring microplastics in German wastewater treatment plant effluent over one year with improved precision and selectivity.
Study Type
Environmental
Fluorescent staining of microplastics as a detection method is consistently gaining importance in microplastics research, as it is fast, easy to use, and requires low technical effort. In this study, a complete procedure was developed, from sample collection to sample processing and detection, to measure microplastics with low cost and time requirements. The developed procedure was tested by measuring the microplastics in the effluent of a German wastewater treatment plant over a period of one year. The results show that the process is especially well suited to investigate temporal variations of microplastic contamination, which requires a large number of samples to be processed. Further, the precision and selectivity of the detection process could be improved by applying newly developed Nile red derivatives for fluorescent staining. A low budget modification of a microscope for fluorescent imaging is compared to a modification with precise optical bandpass filters. A script enabling automated microplastic detection and counting was developed, improving the accuracy and comparability of the process.