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61,005 resultsShowing papers similar to Identification of different plastic types and natural materials from terrestrial environments using fluorescence lifetime imaging microscopy
ClearA promising method for fast identification of microplastic particles in environmental samples: A pilot study using fluorescence lifetime imaging microscopy
Researchers piloted fluorescence lifetime imaging microscopy as a fast method for identifying microplastic particles in environmental samples. The study suggests this technique could simplify microplastic analysis by potentially eliminating the need for extensive extraction steps, enabling more direct identification of plastic particles in complex matrices.
A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)
Researchers developed a rapid microplastic detection method using fluorescence lifetime imaging technology (FLIM) with phasor analysis. The study successfully identified four types of microplastics, both Nile red-stained and unstained, by their unique fluorescence lifetime signatures. The findings suggest that FLIM-based phasor analysis could provide a faster and more accurate approach for microplastic identification compared to conventional spectroscopic methods.
Frequency domain fluorescence lifetime imaging microscopy: A new method to directly identify microplastics in water.
Researchers evaluated frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) as a method to identify ABS, PC, PET, PS, and PVC granulates directly in a 1 cm water layer without filtration or drying. The study found that all five polymer types could be unambiguously identified by their fluorescence lifetimes, establishing FD-FLIM as a promising rapid label-free technique for direct microplastic detection in aqueous samples.
Label-free identification and differentiation of different microplastics using phasor analysis of fluorescence lifetime imaging microscopy (FLIM)-generated data
Researchers developed a label-free method using fluorescence lifetime imaging microscopy (FLIM) combined with phasor analysis to identify and differentiate multiple types of microplastics based on their unique fluorescence lifetime signatures, enabling more efficient microplastic characterization without chemical staining.
Detecting and monitoring the leaching of small (¡ 2 µm) microplastics in soils by fluorescence microscopy
Researchers developed a fluorescence microscopy method to detect and monitor the leaching of small microplastics (under 2 µm) in soils, comparing it against µ-Raman spectroscopy across matrices of varying complexity and demonstrating its applicability for tracking the smallest microplastic fraction in soil systems.
Nile Red lifetime reveals microplastic identity
Researchers developed a fluorescence lifetime imaging approach using the dye Nile Red that can distinguish microplastic particles from biological and mineral debris based on their distinct fluorescence lifetimes, offering faster and more specific microplastic identification than conventional methods.
Polymer Sorting Through Fluorescence Spectra
Identifying which type of plastic a particle is made of is a key step in microplastics research, and this study explored using fluorescence spectroscopy as a faster, cheaper alternative to standard methods. By exposing six common polymers to different light wavelengths and analyzing their fluorescence signatures, the researchers found combinations of wavelengths that could reliably distinguish between plastics like polystyrene, polyamide, and polypropylene. This technique could streamline polymer identification in large-scale environmental monitoring programs.
Determining the influence of variable additive, filler, and dye concentrations in plastics on their fluorescence behavior via spectrometry and FD-FLIM
This study investigated how varying concentrations of additives, fillers, and dyes within plastic materials affect their fluorescence behavior, with the goal of improving fluorescence-based microplastic identification methods. The researchers used spectrometry and fluorescence lifetime imaging microscopy (FD-FLIM) to reveal that these internal variables significantly influence fluorescence signals, which must be accounted for when using fluorescence as a detection technique. More reliable microplastic identification methods are needed to accurately measure contamination levels across diverse environmental and food samples.
Detecting and monitoring the leaching of small (¡ 2 µm) microplastics in soils by fluorescence microscopy
Researchers compared fluorescence microscopy and mu-Raman spectroscopy for detecting the smallest microplastic fraction (1-2 µm) in soils of varying matrix complexity, then used fluorescent microplastics in field conditions to directly measure leaching rates of small particles through soil profiles. The study demonstrated that fluorescence microscopy enables detection and tracking of sub-2 µm microplastics in field soils, providing a practical method for studying the dynamics of the smallest, most mobile plastic fraction in terrestrial environments.
Exploring the Potential of Time-Resolved Photoluminescence Spectroscopy for the Detection of Plastics
Researchers tested time-resolved photoluminescence spectroscopy as a faster alternative to conventional Raman and FTIR spectroscopy for identifying plastic polymers. The technique showed promise for rapid plastic identification, which could speed up microplastic analysis in environmental samples.
Material-Specific Determination Based on Microscopic Observation of Single Microplastic Particles Stained with Fluorescent Dyes
Researchers developed a fluorescence-based technique using commercially available fluorescent dyes to identify the material composition of individual microplastic particles under microscopy, offering a faster first-screening alternative to FT-IR and Raman microspectroscopy. The method was validated on common microplastic types and demonstrated as a practical tool for material-specific determination without requiring specialized spectral expertise.
Towards quality-assured measurements of microplastics in soils using fluorescence microscopy
Researchers applied fluorescence microscopy with Nile Red staining to detect and measure microplastic particles in soil samples and validated the method across eight plastic types and a range of soil types. The method performed well when adapted to account for soil matrix effects. Validated fluorescence microscopy protocols make microplastic analysis in agricultural and environmental soils more accessible and standardized.
Microplastic Analysis in Soil Using Ultra-High-Resolution UV–Vis–NIR Spectroscopy and Chemometric Modeling
Researchers tested a new method using UV-visible-near infrared spectroscopy combined with machine learning to identify microplastics in soil samples. They found the technique could rapidly and accurately distinguish between different plastic polymers and natural soil particles. The study offers a promising alternative to current labor-intensive identification methods, potentially making large-scale microplastic soil monitoring more practical.
The right excitation wavelength for microplastics detection via photoluminescence
Researchers investigated which light wavelengths are best for detecting microplastics using photoluminescence, a technique where particles glow under specific light. Finding the optimal excitation wavelength could make this a practical, low-cost complement to existing microplastic detection tools.
Laser-based spectroscopic techniques: A novel approach for distinguishing aging processes and types of microplastics
Researchers applied laser-based spectroscopic techniques as a novel approach to distinguish between different aging processes and plastic types in microplastic particles, addressing the challenge of identifying weathered plastics that have undergone physical and chemical degradation in the environment.
Quantitative and Qualitative Differences of Common Microplastic Detection Procedures: Nile Red- assisted Fluorescence Microscopy and Confocal Micro-Raman Spectroscopy
Researchers compared Nile Red-assisted fluorescence microscopy and confocal micro-Raman spectroscopy for microplastic detection, finding an overall percentage difference of 421% between methods, with better agreement at smaller particle sizes and Raman spectroscopy offering superior ability to distinguish microplastics from organic matter.
Shedding light on the polymer’s identity: Microplastic detection and identification through nile red staining and multispectral imaging (FIMAP)
Researchers built a multispectral imaging platform called FIMAP that uses fluorescent dye and five different light wavelengths to automatically detect and classify ten types of microplastics with 90% accuracy, while effectively ignoring natural organic matter that typically causes false positives. The system provides a scalable, high-throughput approach for analyzing large environmental samples without needing expensive traditional instruments like infrared spectroscopy.
Towards quality-assured measurements of microplastics in soil using fluorescence microscopy
Researchers tested a fluorescence microscopy method with Nile Red staining for detecting microplastics in different soil types, achieving 80-90% recovery rates for particles larger than 500 micrometers. However, recovery dropped significantly for smaller particles and biodegradable plastics, particularly in clayey soils. The study developed a semi-automated image analysis pipeline and provides quality assurance guidelines for using fluorescence microscopy as a high-throughput microplastic detection method in soil research.
Comparison of Raman and fluorescence microscopy for identification of small (< 2 μm) microplastics in soil
Researchers compared Raman and fluorescence microscopy for detecting very small microplastics (1-2 micrometers) in different soil types. The study found that while Raman microscopy could identify polystyrene in simpler matrices like quartz sand, it failed in clay-rich soils and soils containing organic matter, whereas fluorescence microscopy consistently detected microplastics across all soil types and concentrations tested.
Effects of defined organic layers on the fluorescence lifetime of plastic materials
Researchers measured how defined organic coating layers—simulating environmental weathering—affect the fluorescence lifetime of plastic materials, finding that organic layers alter fluorescence signals in ways that could be exploited for faster detection of microplastics in complex environmental matrices.
Single particle-resolution fluorescence microscopy of nanoplastics
Researchers developed a fluorescence microscopy technique capable of imaging and identifying individual nanoplastic particles. The method enables single-particle resolution detection of nanoplastics, which is a key step toward better quantifying these otherwise invisible particles in environmental samples.
Identification of marine microplastics based on laser-induced fluorescence and principal component analysis
Researchers developed a method to identify different types of marine microplastics using laser-induced fluorescence combined with principal component analysis. The technique successfully distinguished nine types of microplastics based on their fluorescence signatures and could detect microplastic concentrations as low as 0.03% by mass. The study suggests this approach could be a practical tool for rapid microplastic identification in marine environments.
Excitation–Emission Fluorescence Mapping Analysis of Microplastics That Are Typically Pollutants
Researchers introduced a two-dimensional fluorescence excitation-emission mapping method for identifying common microplastics including polystyrene, PET, and polypropylene. Unlike conventional fluorescence approaches that use a single excitation wavelength, this technique captures spectral fingerprints across a range of wavelengths for more reliable identification. The method offers a non-destructive, label-free alternative for detecting microplastic contamination.
Optimization of sample preparation, fluorescence- and Raman techniques for environmental microplastics
Researchers optimized methods for preparing and analyzing environmental microplastic samples using fluorescence staining with Nile Red dye and Raman spectroscopy. The study found that while fluorescence can broadly categorize plastics as polar or non-polar, Raman spectroscopy with a deep-UV laser was needed to reliably identify all polymer types, including those pigmented with carbon black.