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61,005 resultsShowing papers similar to Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
ClearCorrelative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Imaging and quantifying the biological uptake and distribution of nanoplastics using a dual-functional model material
Researchers developed a dual-functional nanoplastic model material that allows both imaging and precise quantification of nanoplastic uptake in biological systems. Using surface-enhanced Raman spectroscopy and inductively coupled plasma mass spectrometry, they could track where nanoplastics accumulated in organisms at high resolution. The tool addresses a major gap in nanoplastic research by enabling more accurate measurement of how these tiny particles interact with living tissues.
Biodistribution and toxicity analysis of polystyrene nanoplastics in mice based on Raman detection
Researchers used surface-enhanced Raman spectroscopy with an optimized gold-silver nanorod substrate to detect and track 20 nm, 100 nm, and 1000 nm polystyrene nanoplastics in mouse lungs, demonstrating accurate biodistribution mapping down to 0.01 mg/mL concentration.
Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
Researchers reviewed advanced optical methods for directly detecting and localizing microplastics in biological tissues, highlighting techniques that can identify particles without extraction or digestion. Optical approaches including Raman mapping and coherent anti-Stokes Raman scattering allow spatial mapping of microplastics in tissue sections.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Spectro‐Microscopic Techniques for Studying Nanoplastics in the Environment and in Organisms
This review examines spectro-microscopic techniques for detecting and characterizing nanoplastics (under 1 um) in environmental and biological matrices, arguing that effective analysis requires combining particle imaging with chemical characterization of the same particles, and highlighting methods capable of simultaneous morphological and chemical identification.
Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
This review surveyed advances in optical techniques for detecting and localizing microplastics directly in biological tissue samples, addressing the challenge that human tissue detection has not kept pace with detection in environmental matrices. Emerging methods including Raman microspectroscopy and CARS microscopy were identified as most promising for tissue-level microplastic identification.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Raman-spektroskopische Charakterisierung von Zellen und Gewebe nach Exposition mit Nanoplastik
Researchers exposed human monocytic THP-1 cells, trophoblasts, and placenta cells to primary and secondary nanoplastic particles at 100 particles/cell in sizes of 200 nm and 60 nm, then used confocal laser scanning microscopy and Raman microspectroscopy to locate and characterize intracellular nanoplastics.
Imaging and quantifying the biological uptake and distribution of nanoplastics using a dual-functional model material
This study used advanced imaging techniques to visualize and quantify nanoplastic uptake and distribution in biological systems, tracking particle translocation from exposure routes into tissues and characterizing intracellular localization.
Super-resolution Raman imaging towards visualisation of nanoplastics
Super-resolution Raman imaging was evaluated as a method to visualize nanoplastics smaller than the conventional diffraction-limited laser spot size, overcoming a key barrier in nanoplastic characterization. The technique extends confocal Raman capabilities into the nanoscale detection range needed for environmental nanoplastic analysis.
Label-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy
Label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy was used to simultaneously visualize internalized microplastics and cellular organelles in live cells without requiring fluorescent staining. The approach enables real-time tracking of plastic particle interactions with intracellular structures, offering new insight into how microplastics behave inside human cells.
Morphological and chemical characterization of nanoplastics in human tissue
Researchers developed methods to visualize and chemically characterize nanoplastics that have accumulated in human tissue samples. They were able to identify plastic particles smaller than one micrometer within tissue using advanced microscopy and spectroscopy techniques. The study provides some of the first direct evidence of nanoscale plastic accumulation in the human body, which is essential for designing future health effects research.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Cortex-Wide, Cellular-Resolution Volumetric Imaging with a Modular Two-Photon Imaging Platform
This paper presents Meso2P, a new two-photon microscope capable of imaging the entire mouse cortex at single-cell resolution — a significant advance in neuroscience instrumentation. Among its demonstrated applications is the ability to track the distribution of micro- and nanoplastic particles in living brain tissue in real time. While primarily a neuroscience tool, its capacity to visualize nanoplastics in the brain non-invasively could become valuable for directly studying how plastic particles move through and accumulate in neural tissue.
Challenges in assessing ecological and health risks of microplastics and nanoplastics: tracking their dynamics in living organisms
Researchers proposed a new method for tracking micro- and nanoplastics in living organisms using fluorescent monomers built directly into the plastic particles during synthesis. Current detection methods require destructive sampling and only provide static snapshots, missing the real-time movement of particles through biological systems. This fluorescent monomer approach is designed to enable continuous, stable imaging of plastic particles as they move through complex biological environments.
Whole-Tissue Distribution Analysis for Visualization of Nanoplastics in the Mouse Brain
Researchers used whole-tissue clearing combined with fluorescence microscopy to visualize the three-dimensional distribution of nanoplastics throughout intact mouse brains without sectioning. This approach revealed nanoplastic accumulation patterns across brain regions that section-based imaging would have missed, demonstrating a valuable method for mapping nanoplastic biodistribution in structurally complex organs.
Spectro‐Microscopic Techniques for Studying Nanoplastics in the Environment and in Organisms
This review examined spectro-microscopic techniques available for detecting and studying nanoplastics in environmental and biological samples. The study highlights that detecting nanoplastics remains challenging because their small size falls below the detection limits of common analytical tools, and their chemical composition is similar to organic matrices, making identification difficult.
Confocal surface-enhanced Raman imaging of the intestine barrier crossing behavior of nanoplastics in Daphnia magna
Using a specially engineered nanoplastic particle visible under confocal Raman imaging, researchers tracked how nanoplastics move from the gut into other organs of the water flea Daphnia magna. The study revealed that nanoplastics can cross the intestinal barrier and translocate to other body parts, providing direct visual evidence of how these particles spread through a living organism and raising concerns about similar processes in other aquatic animals.
Polystyrene nanoplastics demonstrate high structural stability in vivo: A comparative study with silica nanoparticles via SERS tag labeling
Researchers developed a SERS tag labeling technique to track polystyrene nanoplastics in vivo, finding that nanoplastics demonstrate remarkably high structural stability in organisms compared to silica nanoparticles, which degraded more readily.
Correlative SEM-Raman microscopy to reveal nanoplastics in complex environments
Researchers developed a correlative approach combining scanning electron microscopy and Raman microscopy to detect and identify nanoplastics as small as 100 nanometers in complex environmental samples. The method was tested on various matrices and successfully identified individual plastic nanoparticles that would be missed by conventional techniques. The study represents a significant advance in analytical capability for studying the smallest and most challenging size fraction of plastic pollution.
Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
Researchers used spectral focusing hyperspectral stimulated Raman scattering (SRS) microscopy to longitudinally monitor microplastic uptake, size-dependent organ accumulation, and lipid metabolic changes in live zebrafish during development. They found that microplastic exposure disrupted hepatic lipid metabolism and energy homeostasis, with the SRS imaging approach enabling real-time, label-free tracking of microplastics and associated biochemical changes in living organisms.
Limits of the detection of microplastics in fish tissue using stimulated Raman scattering microscopy
This study demonstrated the detection sensitivity of stimulated Raman scattering microscopy for identifying microplastic beads within fish tissue, characterizing how signal-to-noise ratio varies with particle size. The technique provided chemical contrast to distinguish different plastic types within biological tissue without destructive sample preparation.