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61,005 resultsShowing papers similar to Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
ClearAdvancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
Researchers reviewed advanced optical methods for directly detecting and localizing microplastics in biological tissues, highlighting techniques that can identify particles without extraction or digestion. Optical approaches including Raman mapping and coherent anti-Stokes Raman scattering allow spatial mapping of microplastics in tissue sections.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Raman microspectroscopy and laser-induced breakdown spectroscopy for the analysis of polyethylene microplastics in human soft tissues
Researchers developed a combined technique using Raman microspectroscopy and laser-based analysis to detect polyethylene microplastics in human soft tissue samples. The method can identify both the plastic polymer and any associated inorganic elements in tissue. This kind of detection tool is important for understanding whether microplastics accumulate in specific human organs and what health effects they might have.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Raman spectroscopy: Recent advances in fast and reliable microplastic analysis
This review summarized recent advances in Raman spectroscopy for fast and reliable microplastic identification, covering improvements in speed, sensitivity, and automation that are making the technique more practical for routine environmental monitoring. Raman-based methods are increasingly able to identify microplastics in complex environmental matrices including biological tissues.
Morphological and chemical characterization of nanoplastics in human tissue
Researchers developed methods to visualize and chemically characterize nanoplastics that have accumulated in human tissue samples. They were able to identify plastic particles smaller than one micrometer within tissue using advanced microscopy and spectroscopy techniques. The study provides some of the first direct evidence of nanoscale plastic accumulation in the human body, which is essential for designing future health effects research.
Raman spectroscopy: Recent advances in fast and reliable microplastic analysis
This review covered recent advances in Raman spectroscopy for fast and reliable microplastic analysis, with emphasis on its ability to characterize small particles that can penetrate living tissues. The authors highlight improvements in speed and sensitivity that are making Raman techniques more practical for environmental monitoring.
Label-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy
Label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy was used to simultaneously visualize internalized microplastics and cellular organelles in live cells without requiring fluorescent staining. The approach enables real-time tracking of plastic particle interactions with intracellular structures, offering new insight into how microplastics behave inside human cells.
Rapid detection and identification of microplastics from nonchemically treated soil with CARS microspectroscopy
This study developed a coherent anti-Stokes Raman scattering (CARS) microspectroscopy method for rapid in situ detection and identification of microplastics in soil without requiring chemical digestion pretreatment. The approach preserves particle integrity and dramatically reduces analysis time compared to conventional methods.
Detection of microplastics via a confocal-microscope spatial-heterodyne Raman spectrometer with echelle gratings
Researchers built a confocal microscope combined with an echelle-grating spatial-heterodyne Raman spectrometer for detecting microplastics with high sensitivity and resolution. The system achieved spectral resolution approaching 0.67 wavenumbers per centimeter and successfully identified different plastic polymer types. The study demonstrates an efficient and reliable optical detection method that could improve real-time monitoring of microplastic contamination.
Spectro‐Microscopic Techniques for Studying Nanoplastics in the Environment and in Organisms
This review examines spectro-microscopic techniques for detecting and characterizing nanoplastics (under 1 um) in environmental and biological matrices, arguing that effective analysis requires combining particle imaging with chemical characterization of the same particles, and highlighting methods capable of simultaneous morphological and chemical identification.
Raman Spectroscopic Detection of Silicone Leakage in Human Breast and Lymph Node Tissues
This paper is not relevant to microplastics; it uses Raman spectroscopy to detect silicone leakage from ruptured breast implants in human tissue samples.
Direct identification and visualisation of real-world contaminating microplastics using Raman spectral mapping with multivariate curve resolution-alternating least squares
Multivariate curve resolution applied to Raman spectral maps allowed direct identification and visualization of microplastics in complex environmental and tissue matrices without prior physical separation, demonstrating that this chemometric approach reduces analysis time while improving sensitivity for detecting sub-20-micrometer particles.
Advanced Optical Imaging Technologies for Microplastics Identification: Progress and Challenges
This review surveys advanced optical imaging technologies used to identify microplastics and nanoplastics in environmental samples. Researchers compared techniques like Raman spectroscopy, infrared imaging, and fluorescence microscopy, noting their strengths and limitations for detecting increasingly small particles. The study highlights that improving detection methods is essential for accurately monitoring the full scope of plastic pollution.
Misinterpretation in microplastic detection in biological tissues: When 2D imaging is not enough
Researchers demonstrated that 2D Raman imaging alone can misidentify microplastics in biological tissues, showing that 3D confocal Raman imaging is necessary to accurately distinguish microplastic particles from tissue components in mussels.
Optical innovations in microplastic analysis: a critical review of detection strategies
This review surveys advances in optical methods for microplastic detection, including spectroscopic techniques, imaging systems, and sensor technologies. Researchers found that emerging approaches like surface-enhanced Raman spectroscopy combined with machine learning are enhancing automation and detection accuracy. The study identifies the need for standardized protocols and improved techniques to handle the challenges of detecting microplastics in complex environmental and biological samples.
Raman-spektroskopische Charakterisierung von Zellen und Gewebe nach Exposition mit Nanoplastik
Researchers exposed human monocytic THP-1 cells, trophoblasts, and placenta cells to primary and secondary nanoplastic particles at 100 particles/cell in sizes of 200 nm and 60 nm, then used confocal laser scanning microscopy and Raman microspectroscopy to locate and characterize intracellular nanoplastics.
Microplastic identification using Raman microsocpy
Researchers developed and implemented a Raman spectroscopy system for rapid detection and identification of microplastic particles on substrates. The system enables efficient chemical characterization of microplastics found across diverse environmental matrices including ocean, lakes, soil, beach sediment, and human blood.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections
Researchers developed a method using optical photothermal infrared spectroscopy to detect and map microplastics directly within tissue sections without requiring chemical or enzymatic digestion. The study suggests this approach preserves spatial information about where microplastics are located within tissue architecture, overcoming a key limitation of conventional digestion-based methods that can lose some particles.
Raman Spectroscopic Imaging of Human Bladder Resectates towards Intraoperative Cancer Assessment
Researchers used Raman spectroscopy imaging to distinguish between healthy and cancerous human bladder tissue without the need for chemical stains or labels. The technique successfully identified cancer regions in tissue samples from ten patients, using advanced data analysis to map molecular differences. While not directly related to microplastics, this spectroscopy method is also used in microplastic research and demonstrates the power of label-free chemical imaging in medical applications.
Identification and visualisation of microplastics/nanoplastics by Raman imaging (i): Down to 100 nm
Researchers developed an advanced Raman imaging technique capable of identifying and visualizing nanoplastics down to 100 nanometers in size. The study addressed a key analytical gap, as nanoplastic research has been limited by the lack of effective characterization methods, and the new approach offers a way to detect these extremely small particles that may pose greater environmental risks due to their high surface area.
How to Identify and Quantify Microplastics and Nanoplastics Using Raman Imaging?
This paper reviews advances in Raman imaging as a method for identifying and quantifying microplastics and nanoplastics in environmental samples, discussing current protocols, analytical challenges, and the need for standardization.